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Large-leaf yellow tea polysaccharide with blood sugar reducing effect

A technology for lowering blood sugar and large tea, which is applied in the fields of food, health care, and deep-processed tea products, can solve the problems of many impurities, easy destruction of active substances, and low extraction rate.

Inactive Publication Date: 2020-10-20
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Hot water extraction is the traditional extraction method of polysaccharides, which has the disadvantages of low extraction rate, high temperature, easy destruction of active substances, and many impurities.

Method used

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  • Large-leaf yellow tea polysaccharide with blood sugar reducing effect
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  • Large-leaf yellow tea polysaccharide with blood sugar reducing effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of yellow tea polysaccharide with hypoglycemic effect is as follows:

[0021] (1) Pulverize, take 1kg of yellow tea and pour it into a pulverizer to pulverize at 3500r / min. In order to prevent the chemical substances from changing due to high temperature, repeat the operation more than 3 times intermittently, and pass the pulverized powder through a 80-mesh sieve;

[0022] (2) Fermentation, add 1% of the EM bacteria powder into the tea powder and stir evenly, spray 1 times the mass of distilled water and stir, put it into an anaerobic fermentation bag and keep the temperature at 25°C for 9 days of fermentation;

[0023] (3) Extraction, using the deep eutectic solvent extraction method to extract the polysaccharides of yellow tea, the extraction solvent is food-grade choline chloride and glycerin, the mass ratio of the two is 1:2, the liquid-solid ratio is 1:50, the extraction time is 1200min, and the extraction time is 1200min. The temperature is 60...

Embodiment 2

[0030] A preparation method of yellow tea polysaccharide with hypoglycemic effect is as follows:

[0031] (1) Pulverization, take 1.5kg of yellow tea and pour it into a pulverizer and pulverize it at 3500r / min. In order to prevent the high temperature from changing its chemical substances, repeat the operation intermittently for more than 3 times, and pass the pulverized powder through a 60-mesh sieve;

[0032] (2) For fermentation, add 1% of the EM bacteria powder to the tea powder and stir evenly, spray 0.5 times the mass of distilled water and stir, put it into an anaerobic fermentation bag and keep the temperature at 20°C for 7 days of fermentation;

[0033] (3) Extraction, using the deep eutectic solvent extraction method to extract the polysaccharides of yellow tea, the extraction solvent is food grade choline chloride and glycerin, the mass ratio of the two is 1:2, the liquid-solid ratio is 1:20, the extraction time is 150min, and the extraction time is 150min. The temp...

Embodiment 3

[0040] A preparation method of yellow tea polysaccharide with hypoglycemic effect is as follows:

[0041] (1) Pulverization, take 0.5kg of yellow tea and pour it into a pulverizer and pulverize it at 3500r / min. In order to prevent the high temperature from changing its chemical substances, repeat the operation intermittently for more than 3 times, and pass the pulverized powder through a 60-mesh sieve;

[0042](2) For fermentation, add 1% of the EM bacteria powder to the tea powder and stir evenly, spray 0.5 times the mass of distilled water and stir, put it into an anaerobic fermentation bag and keep the temperature at 23°C for 8 days of fermentation;

[0043] (3) Extraction, using the deep eutectic solvent extraction method to extract the polysaccharides of yellow tea, the extraction solvent is food grade choline chloride and glycerin, the mass ratio of the two is 1:2, the liquid-solid ratio is 1:30, the extraction time is 180min, and the extraction time is 180min. The tempe...

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Abstract

The invention discloses a preparation method of large-leaf yellow tea polysaccharide with a blood sugar reducing effect. The preparation method is characterized in that large-leaf yellow tea polysaccharide LYT-2 is prepared by taking large-leaf yellow tea produced from Huoshan as a raw material, performing solid-state fermentation through EM bacteria and carrying out extracting by utilizing a deepeutectic solvent technology; the molecular weight of the polysaccharide compound is 4.38x104 Da, the polysaccharide compound contains 4.98% of binding protein, and the molar ratio of mannose to glucose to arabinose is 2.16:4.83:1 in monosaccharide composition. In-vivo experimental data shows that LYT-2 can obviously reduce the weight and blood sugar level of high-sugar and high-fat mice. The result shows that LYT-2 has good application prospects in the aspects of food, medicine, health care and the like.

Description

technical field [0001] The invention relates to a tea deep-processing product, in particular to an active polysaccharide component with hypoglycemic effect obtained by fermenting yellow tea with probiotic bacteria as a raw material, and belongs to the related fields of food and health care. Background technique [0002] Diabetes, as a "disease of the rich", is a metabolic disease characterized by hyperglycemia. At present, there is no radical cure for diabetes. At present, the treatment of diabetes often relies on drugs to control it. However, the use of drugs often causes great damage to the heart, liver, spleen, stomach, and kidneys. Natural active substances include polysaccharides, proteins, sterols, etc., have a variety of biological activities, have low side effects, and are widely used in the fields of health care and disease treatment, and have broad application prospects. [0003] Yellow Dacha belongs to yellow tea, also known as Wanxi Yellow Dacha. It is produced ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00A61P3/10
CPCA61P3/10C08B37/0003C12P19/04
Inventor 陈彦王志姚程颖潘文娟
Owner ANHUI UNIVERSITY
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