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High-efficiency extraction process for polysaccharide of lotus seeds

An extraction process and polysaccharide technology, applied in the field of high-efficiency extraction process of lotus seed polysaccharides, to achieve the effects of easy operation, high yield and high purity

Active Publication Date: 2012-03-21
福建闽江源绿田实业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing lotus seed extraction methods include ultrasonic extraction, microwave extraction, etc. in addition to decoction.
Fujian Province is one of the main production areas of lotus seeds. A large amount of lotus seed dregs will be produced in the production process of products such as health care drinks, lotus seed juices, etc. This not only causes serious waste of resources, but also requires a lot of money to deal with these side effects. product

Method used

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  • High-efficiency extraction process for polysaccharide of lotus seeds

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Effect test

Embodiment 1

[0033] A high-efficiency extraction process of lotus seed polysaccharide of the present invention, the process flow is shown in figure 1 ,Specific steps are as follows:

[0034] (1) Beat the remaining lotus seed dregs from the production of lotus seed juice with distilled water of 10 times its mass, then pass through a colloid mill with a fineness of 100-120 mesh, and reserve for later use (the lotus seed dregs are provided by Fujian Minjiangyuan Lvtian Industrial Investment and Development Co., Ltd. );

[0035] (2) Add amylase, pectinase and acid cellulase (Zaozhuang Jienuo Bioenzyme Co., Ltd., No. PRO2010113011136) to the lotus seed pulp for hydrolysis, the addition amount is 0.005%, 0.0015%, 0.004%, respectively The hydrolysis temperature is 25°C, and the enzymatic hydrolysis time is 1.5Hr;

[0036] (3) After enzymatic hydrolysis, ultrasonic-assisted extraction is carried out, the ultrasonic power is 350W / kg, and the action time is 6 minutes;

[0037] (4) After ultrason...

Embodiment 2

[0040] A kind of lotus seed polysaccharide high-efficiency extraction process of the present invention, the concrete steps of extraction are:

[0041] (1) Beat the quick-frozen lotus seeds with 15 times the mass of distilled water, and then pass through a colloid mill, with a fineness of 100-120 mesh, for later use (quick-frozen lotus seeds are provided by Fujian Minjiangyuan Lvtian Industrial Investment and Development Co., Ltd.);

[0042](2) Add amylase, pectinase and acid cellulase (Zaozhuang Jienuo Bioenzyme Co., Ltd., No. PRO2010113011136) to the lotus seed pulp for hydrolysis. The hydrolysis temperature is 30°C, and the enzymatic hydrolysis time is 2.0Hr;

[0043] (3) After enzymatic hydrolysis, ultrasonic-assisted extraction is carried out, the ultrasonic power is 400W / kg, and the action time is 8 minutes;

[0044] (4) After ultrasonic-assisted extraction, the extract solution is successively subjected to centrifugation, plate-and-frame filtration and disc centrifugal ...

Embodiment 3

[0047] A kind of lotus seed polysaccharide high-efficiency extraction process of the present invention, the concrete steps of extraction are:

[0048] (1) After soaking and rehydrating the dried lotus, beat it with distilled water 20 times its mass, then pass it through a colloid mill, with a fineness of 100-120 mesh, and reserve it for later use (dried lotus is provided by Fujian Minjiangyuan Lvtian Industrial Investment and Development Co., Ltd.) ;

[0049] (2) Add amylase, pectinase and acid cellulase (Zaozhuang Jienuo Bioenzyme Co., Ltd., No. PRO2010113011136) to lotus seed pulp for hydrolysis, the addition amount is 0.015%, 0.0025%, 0.006%, respectively, enzyme The hydrolysis temperature is 35°C, and the enzymatic hydrolysis time is 2.5Hr;

[0050] (3) After enzymatic hydrolysis, ultrasonic-assisted extraction is carried out, the ultrasonic power is 450W / kg, and the action time is 10 minutes;

[0051] (4) After ultrasonic-assisted extraction, the extract solution is suc...

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Abstract

The invention provides a high-efficiency extraction process for polysaccharide of lotus seeds. The high-efficiency extraction process includes steps of cleaning the lotus seeds, adding distilled water to the lotus seeds for making juice, mixing amylase, pedinase and acidic cellulase for complex enzymolysis at the temperature ranging from 25 DEG C to 35 DEG C, and then producing lotus seed polysaccharide powder after ultrasonic auxiliary extracting, filtering, alcohol precipitating, inorganic ceramic membrane separating and purifying, nanofiltration membrane concentrating and drying. The high-efficiency polysaccharide extraction process is applied to polysaccharide extraction for the lotus seeds by integrating with multiple technologies including complex enzymolysis, ultrasonic auxiliary extraction, multi-grade inorganic ceramic membrane separation and purification and nanofiltration membrane concentration, is easy to operate, pollution-free, low in energy consumption, high in efficiency, fine in healthcare effect and suitable for mass production of polysaccharide of the lotus seeds, and has evident economic benefits, the extraction rate of polysaccharide of the lotus seeds is increased to 3.5% from 1 %, finished-product purity reaches 40%, and produced polysaccharide of the lotus seeds is high in purity.

Description

technical field [0001] The invention relates to a high-efficiency extraction process of lotus seed polysaccharide. Background technique [0002] Lotus seed, also known as Shuizhidan, is an important specialty economic resource and export agricultural product in my country. Polysaccharides are an important part of all living organisms, and widely exist in the cell walls of animals, plants, and microorganisms. They are another important type of biomolecules in organisms besides nucleic acids and proteins. Scientific research has confirmed that sugars have Many biological activities, including anti-tumor, immune, hypoglycemic and anti-viral, etc., and almost no toxic side effects on the body. Therefore, it is of great significance to carry out basic research on lotus seeds and break through the technical bottleneck to promote the development of the current deep processing industry of lotus seeds. In addition to the water decoction method, the existing lotus seed extraction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 刘文聪郑亚凤郑宝东林作兴田继延
Owner 福建闽江源绿田实业投资发展有限公司
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