Preparation method of low molecular glossy ganoderma polysaccharide

A polysaccharide and low molecular technology of Ganoderma lucidum, which is applied in the direction of anti-tumor drugs and drug combinations, can solve the problems of difficult industrialized production, low polysaccharide purity and low specificity, and achieve stable quality, high polysaccharide purity and high extraction rate. Effect

Inactive Publication Date: 2010-05-12
CHINA PHARM UNIV
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a preparation method of low molecular weight Ganoderma lucidum polysaccharide, so as

Method used

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  • Preparation method of low molecular glossy ganoderma polysaccharide
  • Preparation method of low molecular glossy ganoderma polysaccharide
  • Preparation method of low molecular glossy ganoderma polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Example 1 Polysaccharide content detection: anthrone sulfuric acid colorimetric method

[0047] Reagent preparation: Anthrone was dissolved in sulfuric acid at a concentration of 2 mg / ml, and prepared for use on the same day.

[0048] Glucose reference substance solution preparation: take an appropriate amount of dry and constant weight anhydrous glucose, add water to make each 1ml contains about 0.1mg glucose, that is.

[0049] Drawing of the standard curve: Measure 0, 0.1, 0.2, 0.4, 0.6, 0.8, 1.0ml of the glucose reference solution respectively, put them in a stoppered test tube, make up to 1ml with distilled water, add 4ml of anthrone reagent, mix well, boil in a boiling water bath for 10min . After cooling, use tube 0 as a blank control to measure the absorbance at 620 nm. The standard curve was obtained by plotting the absorbance A against the glucose concentration C.

[0050] Determination of the sugar content of the sample: Take about 15mg of the sample, weigh...

Embodiment 2

[0052] Embodiment 2: Preparation of low-molecular ganoderma polysaccharide

[0053] 1. Crushing: Ganoderma lucidum fruiting body (dry product) 500g is pulverized into powder by a pulverizer.

[0054] 2. Water extraction: Weigh 100g of Ganoderma lucidum fruiting body powder, add 1.5L of water, extract at 100°C for 15h, centrifuge to obtain the extract, keep it, add 1.5L of water to the residue, extract at 100°C for 10h, centrifuge to obtain the extract, combine the two extractions liquid.

[0055] 3. Concentration: The extract is concentrated under reduced pressure to 1 / 5 of the original volume, about 700ml.

[0056] 4. Fractional acetone precipitation: slowly add acetone to the concentrated solution, stir while adding, until the ketone content is 35-40%, stand at room temperature for 114 hours, absorb the supernatant (reserved), and centrifuge the lower precipitate to obtain the clear solution, which is combined Add acetone to the supernatant, stir while adding, until the ke...

Embodiment 3

[0058] Embodiment 3: Preparation of low-molecular ganoderma polysaccharide

[0059] 1. Pulverization: Ganoderma lucidum fruiting body (dry product) 1.5kg is pulverized into powder by pulverizer.

[0060] 2. Water extraction: Weigh 1kg of Ganoderma lucidum fruiting body powder, add 20kg of water, extract at 100°C for 20h, centrifuge to obtain the extract, keep it, add 20kg of water to the residue, extract at 100°C for 20h, centrifuge to obtain the extract, combine the two extracts .

[0061] 3. Concentration: The extract is concentrated under reduced pressure to 1 / 5 of the original volume, about 8kg.

[0062] 4. Fractional acetone sedimentation: slowly add acetone to the concentrated solution, stir while adding, until the ketone content is 35-40%, stand at room temperature for 24 hours, absorb the supernatant (retain), centrifuge the lower precipitate to obtain the clear liquid, and combine Add acetone to the supernatant, stir while adding until the ketone content reaches 65-...

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Abstract

The invention mainly relates to a preparation method of low molecular glossy ganoderma polysaccharide, in particular to the preparation method of low molecular ganoderma lucidum polysaccharide. The method comprises the steps of grinding, water extracting, concentrating, grading acetone sinking and centrifugal drying. The preparation method of the low molecular glossy ganoderma polysaccharide is simple, convenient, speedy and efficient, has low cost, few steps, no pollution, and high polysaccharide purity and is suitable for industrialization production.

Description

technical field [0001] The invention relates to a preparation method of red ganoderma polysaccharide, in particular to a preparation method of low molecular weight ganoderma polysaccharide. Background technique [0002] Ganoderma lucidum is a treasure in the treasure house of traditional Chinese medicine. It belongs to Basidiomycetes, Polyporaceae, and Ganoderma genus. It has a medicinal history of more than 2,000 years in my country. A large number of research results at home and abroad show that Ganoderma lucidum has a wide range of pharmacological effects and is toxic. Low. The chemical composition of Ganoderma lucidum is complex, mainly polysaccharides, nucleosides, furans, triterpenes, alkaloids, amino acids, and trace elements. Among them, Ganoderma lucidum polysaccharide is the active ingredient of strengthening the body and strengthening the body, and has anti-tumor effect. [0003] There have been a large number of research reports on the immune regulation of the m...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 黄芳华张陆勇江振洲叶海李纬曹芳
Owner CHINA PHARM UNIV
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