Preparation method of inonotus obliquus polysaccharides

A technology of Inonotus obliquus and polysaccharide is applied in the field of preparing Inonotus obliquus polysaccharide, which can solve the problems of difficult removal of activated carbon powder, influence on product quality and the like, and achieve the effects of low production cost, simple operation and high polysaccharide yield.

Inactive Publication Date: 2019-01-04
DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the activated carbon method is effective in removing pigment, the activated carbon powder in the later stage is not easy to remove, which affects product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Take 1 kg of Inonotus obliquus and use a superfine pulverizer to pulverize it to more than 300 meshes, then freeze the ultrafine powder to -170°C at ultra-low temperature, then place it at room temperature to thaw, repeat the freezing and thawing steps 5 times to break the wall.

[0027] Add 9kg of purified water to the powder after the wall breaking treatment, and extract for 1 hour at a temperature of 83±2°C. After the extraction is completed, the feed liquid is centrifuged with a centrifuge at a speed of 13000r / min, and the centrifugate is collected into a storage tank. The slag is extracted and centrifuged for the second time, and the two centrifuges are combined.

[0028] The centrifugate first uses a microfiltration membrane with a pore size of 0.1 micron to remove large particles of impurities, and then uses an ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons to remove small molecular substances, and collects the liquid retained by the ultra...

Embodiment 2

[0034] Take 1 kg of Inonotus obliquus and use a superfine pulverizer to pulverize it to more than 300 meshes, then freeze the ultrafine powder to -170°C at ultra-low temperature, then place it at room temperature to thaw, repeat the freezing and thawing steps 6 times to break the wall.

[0035] Add 8kg of purified water to the powder after the wall breaking treatment, and extract for 1.5h at a temperature of 83±2°C. After the extraction is completed, the feed liquid is centrifuged with a centrifuge with a rotating speed of 14000r / min, and the centrifugate is collected into a storage tank. The slag is extracted and centrifuged for the second time, and the two centrifuges are combined.

[0036] The centrifugate first uses a microfiltration membrane with a pore size of 0.1 micron to remove large particles of impurities, and then uses an ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons to remove small molecular substances, and collects the liquid retained by ...

Embodiment 3

[0042] Take 1 kg of Inonotus obliquus and use a superfine pulverizer to pulverize it to more than 300 meshes, then freeze the ultrafine powder to -170°C at ultra-low temperature, then place it at room temperature to thaw, repeat the freezing and thawing steps 7 times to break the wall.

[0043]Add 6 kg of purified water to the powder after the wall breaking treatment, and extract for 2 hours at a temperature of 83±2°C. After the extraction is completed, the feed liquid is centrifuged with a centrifuge with a rotating speed of 15000r / min, and the centrifugate is collected into a storage tank. The slag is extracted and centrifuged for the second time, and the two centrifuges are combined.

[0044] The centrifugate first uses a microfiltration membrane with a pore size of 0.1 micron to remove large particles of impurities, and then uses an ultrafiltration membrane with a molecular weight cutoff of 3000 Daltons to remove small molecular substances, and collects the liquid retained ...

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Abstract

The invention discloses a preparation method of inonotus obliquus polysaccharides. The method comprises the following steps: firstly, carrying out superfine grinding on inonotus obliquus, then breaking walls, extracting with hot water and performing centrifugation to obtain an extracting solution, and carrying out the steps of microfiltration and ultrafiltration on the extracting solution, aqueoustwo-phase extraction, macroporous adsorption resin decolorization, nanofiltration desalting and concentration, freeze-drying and the like to obtain an inonotus obliquus polysaccharide product. The preparation method of the inonotus obliquus polysaccharides disclosed by the invention has the advantages that 1, the release of polysaccharide substances in the inonotus obliquus is obviously improvedby using a low-temperature micro-nano wall breaking technology to destroy the cell walls, 2, most of proteins can be separated from the polysaccharides by using an aqueous two-phase extraction technology, to avoid causing influence on the polysaccharides, 3, depigmentation is performed by using macroporous adsorption resin to make the color of the polysaccharide finished product lighter, 4, sodiumcitrate and other inorganic salts mixed in a polysaccharide solution can be removed by using a nanofiltration membrane technology, and the polysaccharide solution is concentrated to avoid the effectof high temperature on polysaccharide activity, and 5, the solvent is safe and pollution-free, the yield is high, and the preparation method is convenient to operate and low in cost.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry and relates to a method for preparing Inonotus obliquus polysaccharides from Inonotus obliquus. In particular, it relates to a method for preparing polysaccharides from Inonotus obliquus by using cell wall breaking technology and two-phase extraction technology. Background technique [0002] Inonotus obliquus is a traditional folk wild medicinal fungus. It is mainly distributed in Russia, Northern Europe, Poland, Hokkaido, Japan, Heilongjiang, Heilongjiang, Jilin Changbai Mountains and other alpine regions between 45° and 50° north latitude. The growth environment is known as "black diamond in the forest" and "fantasy mushroom". Since the sixteenth century, Inonotus obliquus has been widely used in the treatment of various digestive tract cancers (such as gastric cancer, liver cancer, and intestinal cancer), heart disease, and diabetes in many countries around the world. Studies have sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 刘巍
Owner DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD
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