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Method for preparing pure polysaccharose from alfalfa hay

An alfalfa, pure polysaccharide technology, applied in food preparation, animal feed, animal feed and other directions, can solve the problems of imperfect extraction method of alfalfa polysaccharide, unexplored purification technology, energy and time consumption, etc. The effect of leaching time and saving investment

Inactive Publication Date: 2011-10-12
辽宁德宝农牧集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction method of alfalfa polysaccharides is not yet perfect, and the purification technology has not been explored. The traditional extraction method usually requires continuous boiling of grass powder in boiling water for about 2 hours, which consumes energy and time, and the production efficiency is low, and the purity is not guaranteed.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, add 30 times the weight of grass powder and soak in warm water at 30°C for 30 minutes.

[0018] (2) Place in an ultrasonic extractor, raise the temperature to 70°C, then keep the temperature constant, turn on the ultrasonic wave, and apply 60kHz ultrasonic wave at 400W power for 20min, during this period, stir while heating. After the action, filter with a vacuum pump, collect the filtrate, and obtain the polysaccharide extract.

[0019] (3) Use a rotary evaporator to concentrate the extract to 1 / 3 of the original volume at 80°C, and then mix it with an equal volume of n-butanol-chloroform solution (n-butanol and chloroform in a volume ratio of 1:4) than mixing) and mix, shake vigorously in the separatory funnel and let it stand for 30 minutes, discard the lower layer liquid, and repeat the operation 3 times according to this step to obtain the purified polysacch...

Embodiment 2

[0025] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, add 35 times the weight of grass powder and soak in warm water at 35°C for 30 minutes.

[0026] (2) Put it in an ultrasonic extractor, raise the temperature to 75°C, and then turn on the ultrasonic wave at a constant temperature. Under the power of 400W, apply 60kHz ultrasonic wave for 25 minutes, and stir while heating during this period. After the action, filter with a vacuum pump, collect the filtrate, and obtain the polysaccharide extract.

[0027] (3) Use a rotary evaporator to concentrate the extract to 1 / 3 of the original volume at 85°C, and then mix it with an equal volume of n-butanol-chloroform solution (n-butanol and chloroform in a volume ratio of 1:4) than mixing) and mix, shake vigorously in the separatory funnel and let it stand for 30 minutes, discard the lower layer liquid, and repeat the operation 3 times according to this step to obtain the pur...

Embodiment 3

[0033] (1) Crush air-dried or dried alfalfa with a moisture content of less than 15%, pass through a 60-mesh sieve, add 40 times the weight of grass powder, and soak in warm water at 40°C for 30 minutes.

[0034] (2) Put it in an ultrasonic extractor, raise the temperature to 80°C, and then turn on the ultrasonic wave at a constant temperature. Under the power of 400W, apply 60kHz ultrasonic wave for 30 minutes, and stir while heating during this period. After the action, filter with a vacuum pump, collect the filtrate, and obtain the polysaccharide extract.

[0035] (3) Use a rotary evaporator to concentrate the extract to 1 / 3 of the original volume at 90°C, and then mix it with an equal volume of n-butanol-chloroform solution (n-butanol and chloroform in a volume ratio of 1:4 Mixing) to mix, shake vigorously in the separatory funnel and let it stand for 30 minutes, discard the lower layer liquid, and repeat the above steps for 3 times to obtain the purified polysaccharide so...

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Abstract

The invention relates to a method for preparing a pure polysaccharose from alfalfa hay and provides the method for preparing the pure alfalfa polysaccharose, which can be subject to mass production and is simple in process, low in cost and high in efficiency. The method comprises the following steps of: crushing aired or dried alfalfa; soaking the powder in a certain amount of water, wherein the ratio of the powder to the water is 1:20-1:45; heating the mixture at the temperature of 55 to 85 DEG C for 10 to 35 minutes under the action of 40 to 60kHz ultrasonic wave of which the power is 240 to 400W; filtering the mixture and collecting the filtrate; concentrating the obtained filtrate until the volume of the filtrate is reduced to one third; removing proteins from the filtrate with n-butyl alcohol-trichloromethane solution, wherein the volume ratio of n-butyl alcohol to hloromethane is 1 to 4; performing alcohol precipitation on the filtrate with ethanol of which the volume is 2 to 4.5 times that of the filtrate; after standing the filtrate, centrifugating the filtrate at the speed of 3,000 to 5,000 rpm for 10 to 20 minutes; freezing and drying the lower layer of precipitate at the temperature of -70 to -50 DEG C to obtain the e polysaccharose; and separating and purifying the polysaccharose through two kinds of cellulose column chromatography to obtain the high-purity polysaccharose. When the method is compared with the traditional method, the cost is reduced; the production efficiency is greatly improved; and the purity of the obtained polysaccharose product can be up to over 96 percent.

Description

technical field [0001] The invention belongs to a method for extracting plant active components, in particular to a method for preparing pure polysaccharides from alfalfa hay. Background technique [0002] Alfalfa polysaccharide ( Alfalfa Polysaccharides , AP) is a plant polysaccharide extracted from alfalfa, including four components of API, API II, AP III, and API IV. It is a polysaccharide with special biological activity. At present, it has been proved that alfalfa polysaccharide has obvious growth-promoting effect on livestock and poultry within a certain dose range, has the effect of enhancing immune function and anti-infection, and has the effect of treating high cholesterol, and can strengthen blood vessels and prevent arteriosclerosis, thrombosis, and lower blood pressure. Blood sugar, radiation resistance and myocardial infarction, etc. Alfalfa polysaccharide is non-toxic and has significant health effects. In recent years, it has gradually attracted people's att...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L1/09A23K1/16A23K20/163A23L33/125
Inventor 朱宇旌张勇王翀
Owner 辽宁德宝农牧集团有限公司
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