Extraction method of microcoleus vaginatus intracellular polysaccharide

A technology of Microschamoscapensis and intracellular polysaccharide, which is applied in the field of extraction and purification of intracellular polysaccharide, and can solve the problems such as the extraction process and product of intracellular polysaccharide from desert algae, and the extraction method of intracellular polysaccharide from Microcystheca splendens. , to achieve the effect of easy availability of reagents, good removal effect and good purity

Inactive Publication Date: 2016-09-21
XINYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are many reports on the extraction technology of plant polysaccharides and microbial exopolysaccharides. Plant polysaccharide products and some microalgal polysaccharide products (such as spirulina polysaccharides, etc.) products, and there is no report on the extraction method specifically for the intracellular polysaccharides of Microtheca sheaths

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  • Extraction method of microcoleus vaginatus intracellular polysaccharide

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Embodiment 1

[0034] A method for extracting and purifying intracellular polysaccharides of Microtheca sheaths, the steps of which are:

[0035] A. Preparation of Microcoleena sheath algae powder. The algae powder is derived from the large-scale artificial cultivation of Microcoletheca sheaths. BG-11 liquid medium was used as the culture medium, and its components were as follows (per liter): 1.5 g sodium nitrate, 0.04 g dipotassium hydrogen phosphate trihydrate, 0.07 g magnesium sulfate heptahydrate, 0.036 g calcium chloride dihydrate, lemon acid 0.006 g, ferric ammonium citrate 0.006 g, edetate disodium salt 0.001 g, sodium carbonate 0.02 g and 1 mL trace element A5 solution. Among them, the components of A5 solution (per liter) are as follows: 2.86 g of boric acid, 0.039 g of sodium molybdate dihydrate, 0.222 g of zinc sulfate heptahydrate, 0.074 g of copper sulfate pentahydrate, 1.81 g of manganese chloride monohydrate, cobalt nitrate hexahydrate 0.049 g. When preparing 1 liter of BG...

Embodiment 2

[0046] A method for extracting and purifying intracellular polysaccharides of Microtheca sheaths, the steps of which are:

[0047] (1) Grinding the dried Microcolecularis algae flakes (with a fineness of 90 meshes) to obtain Microcoleivoris algae powder;

[0048] (2) Take Microcoletheca sheath algae powder, mix the algal powder and sterile water at a ratio of 1:8, and place it at room temperature for 12 hours; heat it in a water bath (94°C) for 2 hours, filter it with gauze, and take the supernatant; the residue is press The above method was extracted at least 3 times, each time for 2.5 hours, the supernatants were combined, and the polysaccharide extract was stored at 4°C;

[0049] (3) Use a rotary evaporator to concentrate the polysaccharide extract to 1 / 5 of the original volume at 30°C, and store the polysaccharide concentrate at 4°C;

[0050] (4) Precipitate protein with trichloroacetic acid solution at 4°C, so that the content of trichloroacetic acid reaches 4.5% of the ...

Embodiment 3

[0056] A method for extracting and purifying intracellular polysaccharides of Microtheca sheaths, the steps of which are:

[0057] (1) Grinding the dried Microcolebucus algae flakes (with a fineness of 100 mesh) to obtain Microcoleurotheca algae powder;

[0058] (2) Take Microcoletheca sheath algae powder, mix the algal powder and sterile water at a ratio of 1:9, and place it at room temperature for 18 hours; heat it in a water bath (96°C) for 2.5 hours, filter it with gauze, and take the supernatant; the residue is press The above method was extracted at least 2 times, each time for 2 hours, the supernatants were combined, and the polysaccharide extract was stored at 4°C;

[0059] (3) Use a rotary evaporator to concentrate the polysaccharide extract to 1 / 5 of the original volume at 40°C, and store the polysaccharide concentrate at 4°C;

[0060] (4) Precipitate protein with trichloroacetic acid solution at 4°C, so that the content of trichloroacetic acid reaches 4.5% of the t...

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Abstract

The invention discloses an extraction method of microcoleus vaginatus intracellular polysaccharide. The steps include: (1) subjecting microcoleus vaginatus to large-scale culture, then collecting the algal liquid, filtering the algal liquid to obtain an algal piece, and performing air-drying; (2) crushing the algal sheet to obtain algal powder; (3) mixing the algal powder with sterile water to obtain an extracted solution; (4) concentrating the polysaccharide extracted solution to obtain a polysaccharide concentrated solution; (5) using a trichloroacetic acid solution to precipitate protein, performing centrifugation and taking the supernatant; (6) using ethanol to precipitate polysaccharide, and staying the product overnight, conducting centrifugation, retaining the precipitate, dissolving ethanol precipitated crude polysaccharide in distilled water, carrying out running water dialysis, collecting polysaccharide (high molecular weight); (7) after dialysis, adding the dialysate into ethanol; (8) evaporating the ethanol in the precipitated phase to obtain polysaccharide, and conducting freeze drying. The method has the advantages of simple operation, low cost, simple equipment, high extraction rate, good product purity, and safety and reliability, reaches a multi-stage separation and extraction effect, and is suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for extracting and purifying intracellular polysaccharides from Microcoleena sheathing algae powder. Background technique [0002] Since the 1980s, microalgae biotechnology and microalgae metabolic physiology have developed rapidly worldwide, and the basic and applied research of microalgae has been discovered and valued. Microalgae biotechnology, which mainly focuses on the cultivation of microalgae and the development and utilization of its products, is one of the effective ways for people to solve problems such as food, energy, environmental protection and medicine. With the rise and steady development of the microalgae industry, the anti-tumor, anti-virus, anti-radiation, hypoglycemic and immune regulation effects of microalgae polysaccharides are gradually recognized and accepted by people. , Functional food or cosmetics provide a basis for development. my country's m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/04
CPCC08B37/0003C08B37/0084
Inventor 饶本强张少丽田华韩艳婷朱亚利
Owner XINYANG NORMAL UNIVERSITY
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