The invention discloses a dogskin plaster with effects of relaxing muscles and stimulating blood circulation and a preparation method and application of the dogskin plaster. The dogskin plaster is prepared from the following raw material medicines in parts by mass: 10-60 parts of unprocessed radix aconiti, 35-65 parts of rhizoma alpiniae officinarum, 28-49 parts of cortex cinnamomi, 2-30 parts ofangelica sinensis, 15-48 parts of radix stephaniae tetrandrae, 35-50 parts of ephedra, 3-36 parts of flos carthami, 1-15 parts of flos daturae, 4-30 parts of greater celandine herb, 36-58 parts of pericarpium zanthoxyli, 50-60 parts of venenum bufonis, 8-29 parts of semen cleomis, 23-45 parts of herba speranskiae tuberculatae, 10-40 parts of myrrh, 5-25 parts of olibanum, 10-25 parts of oleum anisi stellati, 20-60 parts of rhizoma corydalis, 10-30 parts of rose, 30-50 parts of lignum pini nodi, 2-15 parts of ramulus cinnamomi, 3-20 parts of cortex acanthopancis, 20-60 parts of loofah sponge, 2-30 parts of periostracum cicadae, 8-15 parts of dandelion, 10-30 parts of fructus chaenomelis, 3-20 parts of radix platycodi, 7-25 parts of pinellia ternate, 8-15 parts of dandelion, 10-30 parts of fructus chaenomelis, 20-50 parts of fructus citri sarcodactylis, 5-20 parts of bamboo leaves, 5-20 parts of borneol, 5-10 parts of camphor, 2-5 parts of diphenhydramine hydrochloride, 3-7 parts of methyl salicylate and 4-8 parts of a cutaneous permeable agent.