Composition and preparation method and application thereof

Abstract

The invention discloses a composition, which is prepared from the following raw materials: a mesenchymal stem cell active factor compound, a hydrolyzed placenta extract, hyaluronic acid, collagen, water and an auxiliary reagent. The mesenchymal stem cell active factor compound is prepared from a cell culture supernatant of mesenchymal stem cells and a cell lysis solution through an ultrafiltrationmethod. The hydrolyzed placenta extract is prepared from placenta tissues through decellularization and enzyme digestion methods. The content of active factors in the composition is 0.1-50 micrograms / ml. The invention also discloses a preparation method and application of the above composition. According to the embodiment of the invention, stem cell secretions and accellular placenta tissues areapplied such that the risk of anaphylactic reaction possibly caused by direct use of stem cells is reduced, and effective regulatory factors are all from cell secretions and have higher activity thanrecombinant active factors.

Description

technical field [0001] The invention relates to the technical field of compositions and preparation techniques thereof, in particular to a composition with active factors and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells, vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleukin (IL), collag...

AI Technical Summary

Problems solved by technology

[0005] 1. Many products on the market claim to contain active factors, but they are not actually added, or the activity of recombinant active factors cannot be guaranteed after adding recombinant active factors;

[0006] 2. A single active factor component is often added to the product, but the addition of a single factor cannot play its own role well. For example, EGF, FGF, and IGF require the synergistic effect of multiple factors to effectively play an active role. ;

[0007] 3. The growth factors obtained from active factors derived from genetic engineering often lack chemical modification, the factor activity is not enough, and it is easy to cause allergic reactions;

[0008] 4. Active products are easily inactivated at room temperature and have a short shelf life

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