Composition and preparation method and application thereof

A composition and complex technology, applied in the direction of drug combination, pharmaceutical formula, cosmetic preparations, etc., can solve the problem that a single factor cannot play its own role well, the activity of recombinant factors cannot be guaranteed, and it is easy to cause allergic reactions, etc. Problems, achieve the effect of helping proliferation, powerful regeneration, and reducing risks

Pending Publication Date: 2019-11-29
NOVAPRINT THERAPEUTICS SUZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Many products on the market claim to contain active factors, but they are not actually added, or the activity of recombinant active factors cannot be guaranteed after adding recombinant active factors;
[0006] 2. A single active factor component is often added to the product, but the addition of a single factor cannot play its own role well. For example, EGF, FGF, and IGF re

Method used

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  • Composition and preparation method and application thereof
  • Composition and preparation method and application thereof
  • Composition and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0051] Embodiment one

[0052] An embodiment of the present invention provides a composition, which includes the following raw materials: mesenchymal stem cell active factor complex, hydrolyzed placenta extract, hyaluronic acid and collagen, water, auxiliary reagents;

[0053] The mesenchymal stem cell active factor complex is prepared by ultrafiltration from the cell culture supernatant and cell lysate of mesenchymal stem cells;

[0054] Hydrolyzed placenta extract is prepared from placental tissue by decellularization and enzymatic digestion;

[0055] The active factor content in the composition is 0.1-50ug / ml.

[0056] Among them, mesenchymal stem cells are extracted from animals, allogeneic or autologous, or from placental tissue, umbilical cord, bone marrow, and adipose tissue; that is, human umbilical cord mesenchymal stem cells, adipose-derived mesenchymal stem cells, and bone marrow mesenchymal stem cells can be selected. , placental mesenchymal stem cells, menstrual...

Example Embodiment

[0063] Embodiment two

[0064] The embodiment of the present invention also provides a preparation method of the composition, comprising the following steps:

[0065] S1. Isolation and culture of mesenchymal stem cells;

[0066] S2, subculture and expand the mesenchymal stem cells;

[0067] S3, prepare the composition with active factor, specifically include:

[0068] S301. When the fusion degree of mesenchymal stem cells MSC is 70-95%, stimulate the cells, and collect the supernatant culture solution;

[0069] S302. Centrifuge the collected supernatant culture solution to remove residual cells in the medium, and collect the supernatant; use an ultrafiltration membrane with a filter membrane pore size of 50K to concentrate, and collect the filtrate; take the filtrate and add it to a 3KD ultrafiltration tube , concentrate the culture medium by centrifugation to 10 times, wash with sterile water once, then ultrafilter, and collect the medium concentrate to obtain concentrate ...

Example Embodiment

[0089] Embodiment three

[0090] The embodiment of the present invention also provides the application of the above composition in the preparation of medicaments and / or preparations for wound repair.

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Abstract

The invention discloses a composition, which is prepared from the following raw materials: a mesenchymal stem cell active factor compound, a hydrolyzed placenta extract, hyaluronic acid, collagen, water and an auxiliary reagent. The mesenchymal stem cell active factor compound is prepared from a cell culture supernatant of mesenchymal stem cells and a cell lysis solution through an ultrafiltrationmethod. The hydrolyzed placenta extract is prepared from placenta tissues through decellularization and enzyme digestion methods. The content of active factors in the composition is 0.1-50 micrograms/ml. The invention also discloses a preparation method and application of the above composition. According to the embodiment of the invention, stem cell secretions and accellular placenta tissues areapplied such that the risk of anaphylactic reaction possibly caused by direct use of stem cells is reduced, and effective regulatory factors are all from cell secretions and have higher activity thanrecombinant active factors.

Description

technical field [0001] The invention relates to the technical field of compositions and preparation techniques thereof, in particular to a composition with active factors and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells, vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleukin (IL), collag...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61K8/98A61K8/73A61K8/65A61Q19/00A61K35/28A61K38/18A61K38/30A61K38/20A61K35/50A61K38/01A61P17/02A61K31/728
CPCA61K8/99A61K8/64A61K8/982A61K8/735A61K8/65A61Q19/00A61K35/28A61K38/1808A61K38/1825A61K38/1866A61K38/18A61K38/30A61K38/204A61K38/2053A61K35/50A61K31/728A61K38/014A61P17/02A61K2800/5922A61K2300/00
Inventor 杨熙
Owner NOVAPRINT THERAPEUTICS SUZHOU CO LTD
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