Hydrogel/fibroin nanofiber/neural stem cell integrated transplant for promoting VEGF secretion as well as preparation method and application thereof

A neural stem cell and nanofiber technology, which is applied in the field of hydrogel/silk fibroin nanofiber/neural stem cell integrated transplant and its preparation, can solve the problems of stimulating tumors, affecting the growth or function of tissues and organs, and damaging tissues and cells.

Active Publication Date: 2021-03-16
SHAANXI UNIV OF SCI & TECH +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] However, due to the unstable growth factor, it has short half-life and is extremely easy to degrade in the free state.
In clinical application, when using growth factors to assist the repair process of damaged tissues or organs, it is difficult for growth factors to maintain long-term activity
In addition, delivery of growth factors to the body is not only costly and inefficient, but also may affect the growth or function of normal tissues and organs, for example, a large amount of growth factors may stimulate the formation of tumors, damage tissues and cells

Method used

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  • Hydrogel/fibroin nanofiber/neural stem cell integrated transplant for promoting VEGF secretion as well as preparation method and application thereof
  • Hydrogel/fibroin nanofiber/neural stem cell integrated transplant for promoting VEGF secretion as well as preparation method and application thereof

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preparation example Construction

[0038] The method for promoting VEGF secretion of hydrogel / filament nanofibers / neural stem cell integrated transplantation, including the following steps:

[0039] 1) Preparation of silk fibroin nanofiber bracket

[0040] Silkin is a silk veneer having a moral aqueous molecular aqueous mass concentration of 2% by dissolving, dehydrating, and a mass concentration of 8%: polyethylene oxide PEO (V / V = 4: 1) Solution mixed, wherein the dopamine solution is a component, and the silkin: polyethylene oxide PEO (v / v = 4: 1) solution is b component, A component and B component The mixed volume ratio is 1:10, placed in electrostatic spinning equipment, at 10 kV voltage, 5 mL / h solution flow rate and 15 cm throwing distance (between collectors and needles) (at 25 ~ 30 ° C and 40) Under the relative humidity of%, the spinning fibers are directly collected from a cylinder that is rotatable at 100 rpm / min, and the obtained nano-fibromin fibers will be obtained. Scanning electron mic...

Embodiment 1

[0051] 1) Preparation of silk fibroin nanofiber bracket

[0052] Silin protein is selected from the natural 柞 silk, after dissolving, dehydration, etc., the mass concentration is 2% aqueous solution, and the mass concentration is 8%. The mass concentration is 8%: polyethylene oxide PEO (V / V = ​​4: 1) Solution mixed, wherein the dopamine solution is a component, and the silkin: polyethylene oxide PEO (v / v = 4: 1) solution is b components, and the mixing volume ratio of the A component and the B component is 1:10, placed in electrostatic spinning equipment, at 10 kV voltage, 5 ml hourly solution flow rate and 15 cm throwing distance (between collectors and needles) (relatable at 25 ~ 30 ° C and 40%) Under the humidity. The fibers are directly collected on a cylinders rotating at 100 rpm / min, and the nano-filament fiber bracket will be obtained. Scanning electron microscope observed the diameter diameter diameter diameter of 600 nm. The filamentin nanofiber bracket was determin...

Embodiment 2

[0062] 1) Preparation of silk fibroin nanofiber bracket

[0063] Silin protein is selected from the natural 柞 silk, after dissolving, dehydration, etc., the mass concentration is 2% aqueous solution, and the mass concentration is 8%. The mass concentration is 8%: polyethylene oxide PEO (V / V = ​​4: 1) Solution mixed, wherein the dopamine solution is a component, and the silkin: polyethylene oxide PEO (v / v = 4: 1) solution is b components, and the mixing volume ratio of the A component and the B component is 1:10, placed in electrostatic spinning equipment, at 10 kV voltage, 5 ml hourly solution flow rate and 15 cm throwing distance (between collectors and needles) (relatable at 25 ~ 30 ° C and 40%) Under the humidity. The fibers are directly collected on a cylinders rotating at 100 rpm / min, and the nano-filament fiber bracket will be obtained. Scanning electron microscope observes the diameter diameter diameter diameter diameter of 669 nm. The filamentin nanofiber bracket was...

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Abstract

The invention discloses a hydrogel/fibroin nanofiber/neural stem cell integrated transplant capable of promoting VEGF secretion as well as a preparation method and application thereof, and belongs tothe technical field of biological tissue medical engineering. The method comprises the following step: polysaccharide-based hydrogel/neural stem cell gel are adhered to a fibroin nanofiber scaffold through dopamine to form a polysaccharide-based hydrogel/fibroin nanofiber/neural stem cell integrated transplant, wherein the polysaccharide-based hydrogel is a self-healing injectable polysaccharide-based hydrogel. According to the invention, the polysaccharide-based hydrogel embedded with the neural stem cells is adhered to a gap between fibroin nanofiber scaffolds through dopamine to construct the polysaccharide-based hydrogel/fibroin nanofiber/neural stem cells integrated transplant, so that the cells can be promoted to secrete VEGF and fill the defect part, and provide required microenvironment for adhesion, proliferation and directional differentiation of the neural stem cells into neurons, and the neural stem cells can be applied to the field of treatment of cerebral ischemia injuryand promotion of neural stem cell amplification and differentiation.

Description

Technical field [0001] The invention belongs to the technical field of biological tissue medical engineering, involving a hydrogel / silk nanofiber / neural stem cell integrated transplant and its preparation method thereof, and its preparation method thereof, to promote VEGF secretion. Background technique [0002] Cerebral ischemic injury is one of the main reasons for the death and disability of adults around the world. However, there is no effective treatment. Since the ability of the injured central nervous system self-repair is limited, the functional transplantation of Neural Stem Cells (NSCs) is considered to be cerebral ischemia treatment for the functional transplantation of the neural Stem Cells (NSCs). One of the methods. Neural stem cells are raw cells with long-term self-renewal and differentiation capabilities, with a variety of phenotypic cells to repair the internal ability of the central nervous system, which can be proliferated, migrated, and differentiated int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/58A61L27/18A61L27/20A61L27/22A61L27/38
CPCA61L27/52A61L27/227A61L27/3834A61L27/58A61L27/18A61L27/20A61L2400/12A61L2300/604A61L2430/32A61L2300/422A61L2400/06C08L71/02C08L89/00C08L5/08C08L5/02
Inventor 陈咏梅杨宽郑娟张蓬勃张晓慧李建辉
Owner SHAANXI UNIV OF SCI & TECH
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