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Application of icariin or icarisid II in preparation of medicine for preventing and curing diabetic retinopathy

A technology of diabetic retina and icariin, applied in the fields of diabetes and medicinal chemistry, can solve the problems of weak and insufficient inhibitory effect, etc.

Inactive Publication Date: 2013-07-03
SUZHOU GUANGAO HEALTHCARE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, it was found that the isolated monomer icariside II in Epimedium has a weak inhibitory effect on selective phosphodiesterase type 5 (PDE5), and can enhance the effect of penile erectile function, but the inhibitory effect on PDE5 Weaker than sildenafil (more than two orders of magnitude lower), so it is not enough for one-time, on-demand treatment of erectile dysfunction

Method used

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  • Application of icariin or icarisid II in preparation of medicine for preventing and curing diabetic retinopathy
  • Application of icariin or icarisid II in preparation of medicine for preventing and curing diabetic retinopathy
  • Application of icariin or icarisid II in preparation of medicine for preventing and curing diabetic retinopathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1: Preparation of Icariin, Icariside II and their respective extracts

[0063] Follow steps 1-4 below.

[0064] 1. Preparation of Icariin Extract

[0065] Take 500 grams of dried Epimedium (aerial part) and add 10 liters of anhydrous methanol, heat and extract at 80°C for 3 times, 1 hour each time, 3 times in total. Filtration, recovery of methanol under reduced pressure and concentration into a slurry. Add 4 times the amount of water to disperse, then stand still for 3 hours to remove chlorophyll, a few waxes, etc., extract 4 times of n-butanol, combine the filtrate, and concentrate under reduced pressure. Adsorbed with a 40-60 mesh polyamide column, eluted with 30% ethanol until colorless, and concentrated the eluent under reduced pressure. Add 70% ethanol (1:20) to dissolve, stand still for 8 hours, and filter to obtain icariin. Icariin extracts with different contents were obtained by repeated elution and recrystallization of total icariin and absolute...

Embodiment 2

[0079] Example 2: Research on the Morphological Effects of Icariside II on the Retina of Diabetic Rats

[0080] Experimental purpose: To study the improvement effect of icariside II on retinopathy in diabetic rats.

[0081] Experimental sample: the pure icariside II prepared in Step 4 of Example 1.

[0082] Experimental objects: normal rats and STZ-induced diabetic SD rats.

[0083] Experimental method: 16 2-month-old male SD rats were divided into normal control group (n=8) and diabetic group (n=8). All animal experiments were formally approved by the Animal Protection and Utilization Committee. After fasting for 16 hours in the diabetes group, STZ 60mg / kg was injected intraperitoneally, and the blood sugar induced diabetes model was measured 3 days later. The diabetes model was selected and divided into two groups. The rats in the group were fed with the same dose of normal saline every day for 12 weeks; after the 2-week drug washout period, the rats were euthanized by i...

Embodiment 3

[0087] Example 3: Study on the Effect of Icariin on the Pathological Changes of Diabetic Rat Retina

[0088] Experimental purpose: To study the effect of icariin on the pathological changes of retinal vascular endothelium in diabetic rats.

[0089] Experimental sample: the pure icariin prepared in step 2 of Example 1.

[0090] Experimental objects: normal rats and STZ-induced diabetic SD rats.

[0091] Experimental method: Retinal tissue blocks were cultured to observe the effect of nerve fiber growth; retinal neovascularization and vascular endothelial changes were observed by digesting smears. Specific steps are as follows:

[0092] Twenty-four 2-month-old male SD rats were fasted for 16 hours and intraperitoneally injected with STZ 60 mg / kg. Three days later, the blood sugar was measured to induce diabetes models, and the diabetes models were selected; all animal experiments were formally approved by the Animal Protection and Utilization Committee . Select the diabet...

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Abstract

The invention belongs to the fields of diabetes mellitus and medicinal chemistry, and relates to the application of icariin or icarisid II in preparation of a medicine for preventing and curing diabetic retinopathy, in particular to the application of the icariin, an icariin-containing extract, the icarisid II or an icarisid II-containing extract in preparation of a medicine for preventing and / or curing the diabetic retinopathy. The icariin, the icariin-containing extract, the icarisid II or the icarisid II-containing extract have effective effects on repair or prevention and cure of the diabetic retinopathy, can improve the retinal vessel endothelial cell function remarkably, and can promote the reparative regeneration of optic nerve cells for diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of diabetes and medicinal chemistry, and relates to the use of icariin or icariside II in the preparation of drugs for preventing and treating diabetic retinopathy. Background technique [0002] The WHO reports that the incidence of diabetes is close to 10% of the population. It is estimated that the global incidence of diabetes will reach 300 million people in 2025. Domestic data in recent years show that the blindness rate of diabetic retinopathy is on the rise, which is a major problem that needs to be solved urgently in the medical field today. , has been highly valued by the medical community at home and abroad. The harm of diabetes to human health is not caused by diabetes itself, but mainly by diabetic complications. Diabetic complications are an important cause of disability and death in diabetic patients. Among them, diabetic retinopathy (DR) has become one of the four major causes. One of the causes of blin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/296A61K31/7048A61P3/10A61P25/00A61P27/02
Inventor 辛华卢弘周峰辛钟成
Owner SUZHOU GUANGAO HEALTHCARE CO LTD
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