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Preparation method of polyhydroxyalkanoate/polydopamine composite electrospinning film and electrospinning film

A technology of polyhydroxyalkanoate and polydopamine, which is applied in the field of medical biomaterials, can solve the problems of degradation product cells, tissue damage, structure easy to be damaged by external force, and fast degradation speed, so as to achieve good biocompatibility and prevent tissue damage. Adhesion, promoting effect of tissue repair and regeneration

Inactive Publication Date: 2019-12-20
THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing fiber scaffold preparation materials are absorbable synthetic polymer materials such as PLA and PCL, which have poor mechanical properties, the structure is easily damaged by external forces, and the degradation speed is too fast, and the degradation products have certain damage to cells and tissues. , which limit their application in cell scaffolds, tissue repair
In addition, existing fibrous scaffolds still have problems of adhesion and related complications in the body

Method used

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  • Preparation method of polyhydroxyalkanoate/polydopamine composite electrospinning film and electrospinning film
  • Preparation method of polyhydroxyalkanoate/polydopamine composite electrospinning film and electrospinning film
  • Preparation method of polyhydroxyalkanoate/polydopamine composite electrospinning film and electrospinning film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A polyhydroxyalkanoate / polydopamine composite electrospun membrane, its preparation process is as follows:

[0026] S1. Preparation of poly 3-hydroxybutyrate / 4-hydroxybutyrate (P34HB) electrospun membrane: Weigh 1 g of medical grade P34HB powder (purity greater than 99.5%) and dissolve it in 10 mL of dichloromethane and dimethyl formaldehyde In a compound solvent of amide (DMF) (the volume ratio of dichloromethane and dimethylformamide is 3:7), stir evenly to prepare a P34HB solution with a concentration of 100mg / mL and add it to a 1mL syringe, and the syringe is fixed with a needle nozzle . Under the conditions of room temperature 20°C and humidity 45%, the distance between the needle and the receiver was set to 15 cm, the voltage was 15 kV, and the advancing speed of the syringe was 3 ml / hour, and the electrofilament was collected through ethanol solution. Subsequently, the obtained electrospun silk was washed three times with deionized water, freeze-dried overnight,...

Embodiment 2

[0029] A polyhydroxyalkanoate / polydopamine composite electrospun membrane, its preparation process is as follows:

[0030] S1. Preparation of poly 3-hydroxybutyrate / 4-hydroxybutyrate (P34HB) electrospun membrane: Weigh 0.5 g of medical grade P34HB powder (purity greater than 99.5%) and dissolve it in 10 mL of dichloromethane and dimethyl In a compound solvent of formamide (DMF) (the volume ratio of dichloromethane and dimethylformamide is 6:4), stir evenly to prepare a P34HB solution with a concentration of 100mg / mL and add it to a 1mL syringe. The syringe uses a needle nozzle fixed. Under the conditions of room temperature 20°C and humidity 45%, the distance between the needle and the receiver was set to 15 cm, the voltage was 15 kV, and the advancing speed of the syringe was 2 ml / hour, and the electrofilament was collected through ethanol solution. Subsequently, the obtained electrospun silk was washed three times with deionized water, freeze-dried overnight, and then hot-p...

Embodiment 3

[0033] A polyhydroxyalkanoate / polydopamine composite electrospun membrane, its preparation process is as follows:

[0034] S1. Preparation of poly 3-hydroxybutyrate / 4-hydroxybutyrate (P34HB) electrospun membrane: Weigh 1.5 g of medical grade P34HB powder (purity greater than 99.5%) and dissolve it in 10 mL of dichloromethane and dimethyl In a compound solvent of formamide (DMF) (the volume ratio of dichloromethane and dimethylformamide is 1.5:8.5), stir evenly to prepare a P34HB solution with a concentration of 100mg / mL and add it to a 1mL syringe. The syringe uses a needle nozzle fixed. Under the conditions of room temperature 20°C and humidity 45%, the distance between the needle and the receiver was set to 15 cm, the voltage was 15 kV, and the advancing speed of the syringe was 5 ml / hour, and the electrofilament was collected through ethanol solution. Subsequently, the obtained electrospun silk was washed three times with deionized water, freeze-dried overnight, and then hot...

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Abstract

The invention discloses a preparation method of a polyhydroxyalkanoate / polydopamine composite electrospinning film and the electrospinning film. According to the method, the polyhydroxyalkanoate electrospinning film is prepared by means of an electrospinning technology, and polydopamine (PDA) is grafted onto the surface of a polyhydroxyalkanoate fiber skeleton through chemical modification to prepare the polyhydroxyalkanoate / polydopamine composite electrospinning film. The polyhydroxyalkanoate / polydopamine composite electrospinning film prepared by means of the method has the advantages of great adhesion prevention performance, high biocompatibility and excellent mechanical properties and has a wide application prospect in the biomedical fields of tissue repairing, prevention of tissue adhesion and the like.

Description

technical field [0001] The invention relates to the field of medical biomaterials, more specifically, the invention relates to a preparation method of a polyhydroxyalkanoate / polydopamine composite electrospun membrane with anti-adhesion effect and the electrospun membrane. Background technique [0002] Polyhydroxyalkanoate (PHA) is a linear saturated polyester whose fiber waste can be completely absorbed by the natural environment and has good biodegradability and biocompatibility, while poly 3-hydroxybutyrate / 4- Hydroxybutyrate (P34HB) is the latest generation of biodegradable materials in the PHA family. Fibrous scaffolds prepared based on electrospinning technology have a similar fiber structure to natural extracellular matrix (ECM), and thus are widely used in cell proliferation culture, drug delivery, biosensors and other fields. With the deepening of tissue engineering research, electrospinning technology continues to develop, and different spinning processes and meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M15/37D01F6/84C08G73/06A61L31/14A61L31/06D04H1/728D06M101/32
CPCD06M15/37D01F6/84C08G73/0672A61L31/14A61L31/06A61L31/148D04H1/728D06M2101/32
Inventor 王辉过文泰王怀明杨梓锋秦秀森张迪黄榕康
Owner THE SIXTH AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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