42results about How to "Helps proliferate" patented technology

The invention provides a calcium phosphate/collagen/bone-like apatite three-level bionic bone tissue engineering scaffold and a preparation method thereof. The material of the scaffold possesses components similar to natural bone tissues, and an inorganic/organic/inorganic three-level bionic bone-like netted three-dimensional structure. The preparation method comprises: customizing a calcium phosphate ceramic which possesses a bionic porous structure similar to the natural bone micropore structure, filling collagen prepared by simulated body fluid (SBF) into the porous calcium phosphate ceramic base, low-temperature aging to simulate a biomineralization process, in-situ nucleating on the collagen organic macro-molecule matrix, and self-assembly crystallizing to form a third level bone-like apatite layer structure. The composite scaffold material has bionic material components and bionic microstructure both similar to the natural bone tissues, the mechanical properties and the biological activity of the material are substantially improved, and the composite scaffold material has a wide application prospect.

The invention relates to low-heat-value yoghurt jelly containing superfine full rose powder. The low-heat-value yoghurt jelly is prepared from the following components in parts by weight: 70 parts of fresh milk, 30 parts of cooked soymilk, 15-20 parts of the superfine full rose powder, 8-15 parts of sucrose, 5-10 parts of oligosaccharide, 3-5 parts of a mixed strain fermentation agent, 35-45 parts of whipped cream and 7-10 parts of a mixed stabilizer. The invention further discloses a preparation process of the low-heat-value yoghurt jelly. Experiments prove that the product has the advantages of soft, exquisite, refreshing, lubricating, moderately sour and sweet taste, well-balanced and specific flavor and texture of rose, yoghurt and milk jelly, uniformly pink color, no layering phenomenon and richness in components such as prebiotics, dietary fiber and the like which are beneficial to growth of probiotics.

The invention discloses a preparation method of a corn alcohol-soluble protein nanofiber membrane used for cell culture. The corn alcohol-soluble protein/polyvinyl alcohol nanofiber membrane is a cell culture carrier material, which comprises biodegradable natural polymer corn alcohol-soluble protein and polyvinyl alcohol as a biocompatible high polymer. The transverse dimension of a ribbon-like corn alcohol-soluble protein/polyvinyl alcohol nanofiber is 625 to 3,920 nanometers; and the nanofibers are stacked into the nanofiber membrane randomly. The corn alcohol-soluble protein/polyvinyl alcohol nanofiber membrane is made through electrostatic spinning process, the preparation method is simple and effective, preparation equipment is simple, the stability is high, and indexes such as the diameters of the nanofibers and the porosity of a membrane material and the like can be regulated through changes of the condition of the electrostatic spinning process. The experiment proves that compared with a common PC (polycarbonate) plate, the corn alcohol-soluble protein nanofiber membrane material contributes to proliferation of mouse embryonic stem cells, and the diameter of the stem cell clone cultured for 5 days reaches 100 micrometers. Moreover, the nanofiber membrane can be recycled after being steamed and pressed for 30 minutes at 120 DEG C.

The invention provides a novel dual-layer nerve conduit. The novel dual-layer nerve conduit consists of an electrospin decellularization pig nerve substrate conduit layer, and a PLCL layer arranged onthe outer layer of the electrospin decellularization pig nerve substrate conduit layer. Besides, the invention provides a preparation method of the novel dual-layer nerve conduit. The preparation method comprises the following steps of (1) pretreating pig nerves; (2) performing decellularization on the pretreated pig nerves; (3) performing crushing and drying treatment on nerves after being subjected to decellularization; (4) performing electrostatic spinning on the dual-layer nerve conduit layer; and (5) performing post-treatment on the dual-layer nerve conduit layer. The novel dual-layer nerve conduit prepared by the method disclosed by the invention has good biocompatibility and favorable machinery support; an inner-layer decellularization matrix contains various kinds of functional proteins, including collagen, proteoglycan, glycosaminoglycans and nerve growth factors, proliferation, migration and axon growth of schwann cells can be promoted, and the outer-layer PLCL can also effectively prevent hyperplasia of fibrous connective tissue and invasion of surrounding tissue.

The invention discloses an electrochemical method for preparing a strontium-doped hydroxyapatite ridge rodlike structure coating on a titanium surface. The method comprises the steps of preparing high and low-concentration electrolyte solutions containing Ca, Sr and P; heating the two electrolyte solutions to a certain temperature and keeping the temperature by using constant-temperature heating equipment; taking a platinum sheet as a positive electrode and pure titanium as a negative electrode base body, and soaking the two electrodes in the electrolyte solutions during depositing; depositing in the high and low-concentration electrolyte solutions for a certain time to finally obtain the strontium-doped hydroxyapatite ridge rodlike structure coating; after the deposition is finished, taking out the base body, cleaning and drying. The strontium-doped hydroxyapatite ridge rodlike structure coating is prepared by adopting the method, the specific surface area of the coating is enlarged, and the coating is relatively good in wetting property; meanwhile, the dissolution characteristic of the coating is changed due to strontium ion doping, and a new direction is opened up for preparation and application of a modified hydroxyapatite bio-coating.

The invention discloses a method for producing microalgae oil by Monoraphidium. The method comprises (1) preparing a microalgae seed liquid, wherein the microalgae is Monoraphidium sp. SS-06 and has a preservation number of CGMCC No. 10765, (2) inoculating an N element-rich microalgae culture medium with the Monoraphidium seed liquid, carrying out culture until a logarithmic growth phase, carrying out standing and sedimentation, discharging the supernatant and acquiring microalgae cells, (3) inoculating a low N element content microalgae culture medium with the microalgae cells, adding an ADP glucose pyrophosphatase inhibitor into the culture medium and carrying out continuous illumination culture until a stable period to obtain oil-rich microalgae cells. The method is conducive to proliferation of microalgae cells and accumulation of oil, can produce microalgae cells with high oil content and has the characteristics of simple processes, low cost and high yield.

The invention provides a micromolecular nutrition agent capable of effectively promoting proliferation of beneficial intestinal bacteria. Pulp of yacon, pulp of burdock and pulp of Chinese yams are taken as fermenting raw materials, and are fermented by taking saccharomyces cerevisiae, lactobacillus acidophilus and lactobacillus plantarum as fermenting agent to obtain the micromolecular nutritionagent. The invention further provides a preparation method of the micromolecular nutrition agent. According to the micromolecular nutrition agent capable of effectively promoting proliferation of thebeneficial intestinal bacteria and the preparation method of the micromolecular nutrition agent, the yacon, the burdock and the Chinese yams are taken as the raw materials, and are fermented through the saccharomyces cerevisiae, the lactobacillus acidophilus and the lactobacillus plantarum, the fermenting period can be shortened remarkably, fermenting steps are reduced, a fermented product is richin oligosaccharide, mycoprotein and various vitamins, and supplementation and proliferation of probiotic groups in intestinal tracts of human bodies can be facilitated.

The invention belongs to the field of foods, and particularly relates to a steamed bun containing soybean oligosaccharides and a production method of the steamed bun. The steamed bun containing oligosaccharides, disclosed by the invention, comprises the following raw materials in parts by weight: 1-5 parts of yeast, 800-1100 parts of flour, 300-900 parts of soybean dietary fibers, 0.5-4 parts of dietary alkali, 80-110 parts of sourdough and 30-150 parts of soybean oligosaccharides. According to the steamed bun disclosed by the invention, the soybean oligosaccharides and the soybean dietary fibers are added and are in joint synergistic reaction, so that the effect of loosening bowel to relieve constipation is good, and eliminating of diseases of patients suffering from constipation is facilitated. The soybean dietary fibers are difficult to dissolved with flour, the method disclosed by the invention solves the technical problem, and the soybean dietary fibers and the flour are dissolved well, so that a present situation that nutrient components of the steamed bun are relatively single is changed; besides, the steamed bun disclosed by the invention is particularly suitable for middle-aged and aged patients suffering from constipation, pregnant women, and crowds with large working pressure and few movement to eat.

The invention discloses a feed additive for improving the growth performance of a nursery pig. The invention discloses a preparation method of the feed additive for improving the growth performance ofthe nursery pig, wherein the preparation method comprises the following steps: mixing Fuzhuan tea, jujube, star anise and raw ginger, crushing, adjusting the water content, adding a compound enzyme preparation, mixing evenly, carrying out enzymatic hydrolysis, and filtering to obtain a first material and an enzymatic hydrolysate; adding the first material into absolute ethyl alcohol, soaking, andcarrying out ultrasonic assisted reflux extraction to obtain an extract liquid and a filter residue; adding the filter residue to water, soaking, cooking and filtering to obtain a filtrate; mixing the enzymatic hydrolysate, the extract liquid and the filtrate, and drying at low temperature to obtain a second material; adding the second material, excipients and a cladding wall material into water,mixing evenly and spray-drying to obtain the feed additive for improving the growth performance of the nursery pig. The invention discloses an application of the feed additive for improving the growth performance of the nursery pig.

The invention discloses a normal bud proliferation method of gingko ornamental varieties. Normal bud proliferation is performed on excellent gingko varieties by adopting a tissue culture method in the invention, so that the reproduction rate of the excellent gingko varieties is increased and the production requirements can be met. In the invention, by adopting the feature that the axillary bud of gingko is liable to germinate, a tender stem segment with an axillary bud is used as an explant, the axillary bud is promoted to germinate by the tissue culture method, so as to proliferate the normal buds of the excellent gingko varieties, keep the excellent properties of the female patient and provide a large amount of effective tender branches with certain lengths, thicknesses and degrees of lignifications to provide materials for microbody cuttings. The method is used for proliferating the normal buds of gingko, and is simple and feasible to operate.

The invention provides a preparation method of 3D printed silk protein hydrogel. The preparation method includes the following steps: preparing pre-crosslinked silk protein hydrogel and 3D printed silk protein hydrogel, and specifically includes adding double-bond modified cyclodextrin and a photoinitiator into a silk protein solution, stirring to obtain single-network cross-linked silk protein hydrogel, and irradiating the single-network cross-linked silk protein hydrogel by UV light to form the pre-crosslinked silk protein hydrogel; crosslinking tyramine radicals on molecular chains of the cyclodextrin and silk protein in fiber filaments through 3D printing, and performing layer-by-layer stacking to form the 3D printed silk protein hydrogel. The invention further provides the 3D printedsilk protein hydrogel. The preparation method of the 3D printed silk protein hydrogel can effectively solve the problem of inter-layer separation of the 3D printed silk protein hydrogel, the obtainedsilk protein hydrogel is beneficial to adhesion and spreading of cells to materials in the cell culture process, and application of silk protein hydrogel materials to cell migration and proliferationin the cell culture process is facilitated.

The invention discloses a method for producing starch by Monoraphidium. The method comprises (1) preparing a microalgae seed liquid, wherein the microalgae is Monoraphidium sp. SS-06 and has a preservation number of CGMCC No. 10765, (2) inoculating an N element-rich microalgae culture medium with the Monoraphidium seed liquid, carrying out culture until a logarithmic growth phase, carrying out standing and sedimentation, discharging the supernatant and acquiring microalgae cells, (3) inoculating a low N element content microalgae culture medium with the microalgae cells, adding an ADP glucose pyrophosphatase inhibitor into the culture medium and carrying out continuous illumination culture until a stable period to obtain starch-rich microalgae cells. The method is conducive to proliferation of microalgae cells and accumulation of starch, can produce microalgae cells with high starch content and has the characteristics of simple processes, low cost and high yield.

The invention relates to a bioreactor process of a chick embryo cell rabies virus. Primary chick embryo cells are cultured in a bioreactor with a fixed bed basket type stirring system by using a sheetcarrier and a serum-free culture medium, and rabies virus harvesting liquid with high titer and high antigen is prepared through specific in-tank cell density, inoculation amount of CTN chick embryocell vaccine strains-CTNCEC25 strains (CGMCC No.6510) and set parameters of the bioreactor. The virus titer is averagely not lower than 7.0 lgFFU/mL, the highest virus titer can reach 8.7 lgFFU/mL, the antigen content is averagely not lower than 2.0 IU/mL, the highest antigen content can reach 4.0 IU/mL, the harvesting liquid can reach 2-3 effective culture volumes, the labor intensity and the production cost are greatly reduced, the production efficiency is greatly improved, and a culture mode for producing rabies viruses through primary cell large-scale culture is created.

The present invention discloses a phagostimulant produced by using fish soluble slurry. The phagostimulant is obtained by fish soluble slurry microorganism removing, defatting, enzymolyzing, fermenting, fishy smell increasing and shaping. Beneficial effects are as follows: residual fat in the fish soluble slurry is completely enzymolyzed and a ventilation is conducted to enable fatty acids produced by the enzymolyzing to be completely oxidized; proteins are completely hydrolyzed into oligopeptides, amino acids and small molecules beneficial to digestion and absorption of fish; active polypeptides can affect proliferation of fish intestinal microorganisms, activate pepsinogen and improve a feed conversion rate; trimethylamine oxide dehydrate is significantly in fishy smell increasing functions and can maximally play an attraction function of the phagostimulant; and a finished product of the phagostimulant is powdery, easy for storage and transportation, long in shelf life under a normal temperature condition, reasonable in design and convenient in operations, turns a waste of the fish soluble slurry into treasures, reduces harms of a production of fish meal on environment, and improves resource utilization rate and added value.

The invention discloses a preparation method of a bletilla striata polysaccharide composite cytoskeleton. The method comprises the steps that (1) bletilla striata polysaccharide is prepared; (2) oxidized konjac glucomannan is prepared; (3) the bletilla striata polysaccharide and the oxidized konjac glucomannan are taken to be added into distilled water, heating, stirring and uniform mixing are carried out, sufficient dissolution is carried out, then bubbles are removed by standing overnight at 4 DEG C, pre-freezing, freezing and vacuum drying are carried out to obtain the bletilla striata polysaccharide composite cytoskeleton. The preparation method of the bletilla striata polysaccharide composite cytoskeleton has the advantages that the cytoskeleton with a controllable void ratio and excellent network mechanical property is obtained by blending the bletilla striata polysaccharide and the oxidized konjac glucomannan, and the problems of the single raw material source, inhomogeneous porosity and uncontrollability of an existing cytoskeleton can be effectively solved; the provided bletilla striata polysaccharide composite cytoskeleton has good biocompatibility, tissue inductivity andnetwork mechanical property, has a good market prospect in the field of tissue engineering, and is conductive to the migration and proliferation of stem cells, vascular endothelial cells and the likeand the angiogenesis.

The invention discloses honey helping digestion and a preparation technology thereof, and belongs to the field of food processing. According to the technical scheme, the honey is prepared from, by weight, 100 parts of honey, 8-12 parts of grape juice, 12-16 parts of lyophilized lactobacillus powder, 1-7 parts of bitter gourd powder, 2-6 parts of rose pollen, 2-12 parts of fructo-oligosaccharide and 3-7 parts of chitosan oligosaccharide. The effects of enriching the ingredients of honey and promoting digestion are achieved.

The present invention relates to the technical field of artificial prosthesis for medical treatment, and specifically refers to a bionic artificial spinal prosthesis with flanges; Both ends of the body are provided with threaded holes. The bionic prosthesis and the flange body are an integrated structure with micro-holes constructed by 3D printing technology. The lower end surface of the bionic prosthesis and the upper end surface of the flange body are respectively penetrated; the structure of the present invention is reasonable, the upper end of the bionic prosthesis has a flange body, and the contact surfaces between the two and the upper and lower vertebrae are microporous structures, which are conducive to bone cell proliferation and Bone fusion; the bottom support and reinforcement ring at the fusion end construct a metal bearing surface, improve the compressive strength and twist resistance of the bionic prosthesis through the support beam, and improve the stability and reliability of the prosthesis; the porous prosthesis constructed by 3D printing technology Bionic prostheses can effectively promote the growth of bone cells and promote the healing and recovery of diseased parts.

The invention belongs to the field of medicine, and particularly relates to application of a traditional Chinese medicine composition in preparing a drug for treating diseases caused by mucosal injury. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 80 parts of rhizoma atractylodis, 80 parts of tangerine peel, 80 parts of mangnolia officinalis, 120 parts of radix angelicae dahurioae, 120 parts of poria cocos, 120 parts of areca peel, 80 parts of unprocessed rhizoma pinelliae, 10 parts of licorice root extract, 0.8 part by volume ofpatchouli oil, and 0.4 part by volume of perilla leaf oil. The traditional Chinese medicine composition of the invention adopts the traditional Chinese herbs as raw materials, is less in toxic and side effects, facilitates the proliferation of mucosal cells, and can promote the restoration of the injured mucosa. Therefore, the traditional Chinese medicine composition of the invention can be used for preparing the drug for treating the diseases caused by the mucosal injury.

The invention provides a method for preparing an active probiotic milk beverage. High fructose corn syrup, lactobacillus casei or lactobacillus paracasei freeze-dried bacteria powder, xylitol, erythritol and the like are added into skimmed milk; the prepared active probiotic milk beverage can regulate the balance of intestinal flora, and added probiotics help the proliferation of intestinal probiotics and promote the absorption of nutrients; both xylitol and erythritol are functional sugar alcohol, and have the characteristics of low sweetness, low calorie, no tooth-decaying property and little effect on blood glucose; therefore, the active probiotic milk beverage can be used as a decayed-tooth preventing food, is also suitable for patients with diabetes, hyperlipidemia and obesity, and has wide consumer groups.

The invention provides an orthopedic wound antibacterial protective film and a preparation method thereof. The antibacterial protective film comprises an endogenous antibacterial protective film and an exogenous antibacterial protective film, wherein the endogenous antibacterial protective film is mutually bonded and is in contact with a wound, and the exogenous antibacterial protective film is incontact with the external environment. The endogenous antibacterial protective film is a pH-sensitive degradable high-molecular polymer film loaded with an antibacterial agent, and the pH-sensitive degradable high-molecular polymer comprises acid-sensitive chemical bonds. When inflammation is generated in the wound due to endogenous bacterial infection, the pH value of the wound is reduced, so that the acid-sensitive chemical bonds are broken, and the loaded antibacterial agent is slowly released to inhibit the endogenous bacterial infection. The exogenous antibacterial protective film is a chitosan film grafted with graphene and is used for blocking and inhibiting exogenous bacterial infection. The graphene is connected with the chitosan through chemical bonds and thus cannot enter a human body, so that exogenous bacteria can be effectively inhibited. Therefore, only when endogenous infection occurs, the endogenous antibacterial protective film releases the antibacterial agent, so that the antibacterial agent entering the human body is obviously reduced.

The invention belongs to the technical field of algae, and discloses a preparation method of heterogloea powder. The preparation method comprises the following steps of step 1) preparing a heterogloeaculture solution; step 2) performing high-density culturing; and step 3) preparing algae powder. According to the method disclosed by the invention, the algae cells are precipitated through flocculation, and then the algae powder is prepared through freeze drying and crushing, so that the heterogloea powder can be applied to aquaculture, and the market application value is extremely high.