3D printed silk protein hydrogel and preparation method thereof

Abstract

The invention provides a preparation method of 3D printed silk protein hydrogel. The preparation method includes the following steps: preparing pre-crosslinked silk protein hydrogel and 3D printed silk protein hydrogel, and specifically includes adding double-bond modified cyclodextrin and a photoinitiator into a silk protein solution, stirring to obtain single-network cross-linked silk protein hydrogel, and irradiating the single-network cross-linked silk protein hydrogel by UV light to form the pre-crosslinked silk protein hydrogel; crosslinking tyramine radicals on molecular chains of the cyclodextrin and silk protein in fiber filaments through 3D printing, and performing layer-by-layer stacking to form the 3D printed silk protein hydrogel. The invention further provides the 3D printedsilk protein hydrogel. The preparation method of the 3D printed silk protein hydrogel can effectively solve the problem of inter-layer separation of the 3D printed silk protein hydrogel, the obtainedsilk protein hydrogel is beneficial to adhesion and spreading of cells to materials in the cell culture process, and application of silk protein hydrogel materials to cell migration and proliferationin the cell culture process is facilitated.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a 3D printing silk protein hydrogel and a preparation method thereof. Background technique [0002] Silk protein can be obtained from cotton or silk glands. Compared with natural biological macromolecules such as hyaluronic acid and gelatin, silk protein has higher mechanical strength; in addition, compared with synthetic polymers, silk protein molecular chains have cell recognition sites , contribute to the adhesion and proliferation of cells on silk protein hydrogel materials during cell culture. Its degradation process can be divided into enzymatic and non-enzymatic hydrolysis. Due to the existence of the crystalline structure formed by molecular chains, its degradation process is slow and helps tissue regeneration. Therefore, silk protein is often used as one of the raw materials for preparing hydrogels. one. 3D printing technology helps to prepare hydrogel mat...

AI Technical Summary

Problems solved by technology

The commonly used raw materials for 3D printing hydrogels are mainly gelatin or sodium alginate materials. In order to ensure that the overall structure does not collapse during the printing process, a faster gelation speed is required, but too fast gelation speed usually leads to printing The resulting hydrogel material layer is not bonded between layers, resulting in layer separation.

There are tyrosine residues on the silk protein molecular chain, which can be used as guest molecules to interact with cyclodextrin to construct a three-dimensional hydrogel network. The host-guest interaction is a non-covalent bond interaction and can be used to prepare self-healing However, due to the large steric hindrance effect in the reaction process between macromolecules and macromolecules, which affects the occurrence of host-guest interaction, the resulting hydrogel will affect the self-healing effect due to the lack of non-covalent bond pairs.

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