Preparation method and application of heterogloea powder

A technology of algae and algae powder, applied in the field of algae, can solve the problems of large differences in algae culture conditions, inability to apply and copy, and poor environmental friendliness, and achieve high market application value, improve immunity, and promote cell division. Effect

Active Publication Date: 2020-07-10
杨凌海思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has defects such as high cost, insufficient clean water, easy to carry pollutants, insufficient density, and

Method used

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  • Preparation method and application of heterogloea powder
  • Preparation method and application of heterogloea powder
  • Preparation method and application of heterogloea powder

Examples

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Example Embodiment

[0031] Example 1

[0032] The preparation method of isoglobin powder includes the following steps:

[0033] Prepare the culture solution of Isoglobin: Improve the f / 2 medium, as follows: add ammonium bicarbonate and brassinolide to the f / 2 medium, and control the concentration of ammonium bicarbonate and brassinolide to 50mg respectively / L and 0.03mg / L;

[0034] High-density culture: Select vigorously growing, non-polluting Heterogliceae and connect it to the culture tank containing Heterogliceae culture solution. The inoculation density is 5 million / ml, the temperature is controlled at 25℃, and the red light is irradiated with red light. Electromagnetic radiation of 720nm wavelength, the light intensity is 45μmol / (m 2 .S), the light-dark time ratio is 12:12, the air flow is 0.5vvm, and the algae solution is obtained after 9 days of cultivation; during the entire cultivation process, the salinity is controlled by adding a sodium chloride aqueous solution with a concentration of 2mo...

Example Embodiment

[0036] Example 2

[0037] The preparation method of isoglobin powder includes the following steps:

[0038] Prepare the culture solution of Isoglobin: Improve the f / 2 medium, as follows: Add ammonium bicarbonate and brassinolide to the f / 2 medium, and control the concentration of ammonium bicarbonate and brassinolide to 30 mg respectively / L and 0.02mg / L;

[0039] High-density culture: Select vigorously growing, non-polluting Heterogliceae and connect it to the culture tank containing Heterogliceae culture solution. The inoculation density is 5 million / ml, the temperature is controlled at 25℃, and the red light is irradiated with red light. 740nm wavelength electromagnetic radiation, the light intensity is 55μmol / (m 2 .S), the light-dark time ratio is 14:10, the air flow rate is 0.6vvm, and the culture is 8 days; during the entire culture process, the salinity is controlled to 25 by adding a sodium chloride aqueous solution with a concentration of 3mol / L;

[0040] Prepare algae powde...

Example Embodiment

[0041] Example 3

[0042] The preparation method of isoglobin powder includes the following steps:

[0043] Prepare the culture solution of Isoglobin: Improve the f / 2 medium, as follows: Add ammonium bicarbonate and brassinolide to the f / 2 medium, and control the concentration of ammonium bicarbonate and brassinolide to 40mg respectively / L and 0.025mg / L;

[0044] High-density culture: Select vigorously growing, non-polluting Heteroglioma and connect it to the circular track pond containing Heteroglioma culture solution, the inoculation density is 5 million / ml, the temperature is controlled at 25℃, red light irradiation, red light Electromagnetic radiation with a wavelength between 730nm, the light intensity is 50μmol / (m 2 .S), the light-dark time ratio is 10:14, the air flow is 0.5vvm, and the culture is 7 days; during the entire culture process, the salinity is controlled to 23 by adding a sodium chloride aqueous solution with a concentration of 2mol / L;

[0045] Prepare algae powde...

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Abstract

The invention belongs to the technical field of algae, and discloses a preparation method of heterogloea powder. The preparation method comprises the following steps of step 1) preparing a heterogloeaculture solution; step 2) performing high-density culturing; and step 3) preparing algae powder. According to the method disclosed by the invention, the algae cells are precipitated through flocculation, and then the algae powder is prepared through freeze drying and crushing, so that the heterogloea powder can be applied to aquaculture, and the market application value is extremely high.

Description

technical field [0001] The invention belongs to the technical field of algae, and in particular relates to a preparation method and application of algae powder. Background technique [0002] Microalgae are rich in nutrients, and many algae contain high protein, vitamins and trace elements necessary to maintain the growth of aquatic product seedlings, which can obviously promote various physiological functions of seedlings. For example, vitamin E can promote the phagocytosis of aquatic animal macrophages, improve the activity of complement, and enhance the immunity of aquatic animal larvae; adding anabaena powder to shrimp compound feed can significantly improve the growth rate and disease resistance of shrimp. Therefore, fine algal species such as Anabaena and Chlorella have good application prospects in the cultivation of fish and shrimp fry. Adding a certain proportion of unicellular algae to the compound feed as the bait in the early stage of fish and shrimp nursery, the...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02A23K50/80A23K10/16C12R1/89
CPCC12N1/12C12N1/02A23K50/80A23K10/16Y02A40/81
Inventor 童文连鲍君美张杨康
Owner 杨凌海思生物科技有限公司
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