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Preparation method and application of heterogloea powder

A technology of algae and algae powder, applied in the field of algae, can solve the problems of large differences in algae culture conditions, inability to apply and copy, and poor environmental friendliness, and achieve high market application value, improve immunity, and promote cell division. Effect

Active Publication Date: 2020-07-10
杨凌海思生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method has defects such as high cost, insufficient clean water, easy to carry pollutants, insufficient density, and poor environmental friendliness.
[0005] The culture conditions of different algae are quite different, and cannot be applied and copied

Method used

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  • Preparation method and application of heterogloea powder
  • Preparation method and application of heterogloea powder
  • Preparation method and application of heterogloea powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation method of algae powder, it comprises the steps:

[0033] Prepare Heterococcus culture medium: improve the f / 2 medium, specifically as follows: add ammonium bicarbonate and brassinosteroid to the f / 2 medium, and control the concentrations of ammonium bicarbonate and brassinosteroid to be 50mg respectively / L and 0.03mg / L;

[0034] High-density culture: Select vigorously growing and non-polluting Heterococcus and insert them into the culture pool containing Heterococcus culture solution. The inoculation density is 5 million / ml, the temperature is controlled at 25°C, and red light is irradiated. Electromagnetic radiation with a wavelength of 720nm, the light intensity is 45μmol / (m 2 .S), the light-to-dark time ratio was 12:12, the air flow rate was 0.5vvm, cultured for 9 days, and the algae liquid was obtained; during the whole culture process, the salinity was controlled by adding a sodium chloride aqueous solution with a concentration of 2mol / L. twenty ...

Embodiment 2

[0037] The preparation method of algae powder, it comprises the steps:

[0038] Prepare Heterococcus culture medium: improve the f / 2 medium, specifically as follows: add ammonium bicarbonate and brassinosteroid to the f / 2 medium, and control the concentrations of ammonium bicarbonate and brassinosteroid to be 30mg respectively / L and 0.02mg / L;

[0039] High-density culture: Select vigorously growing and non-polluting Heterococcus and insert them into the culture pool containing Heterococcus culture solution. The inoculation density is 5 million / ml, the temperature is controlled at 25°C, and red light is irradiated. Electromagnetic radiation with a wavelength of 740nm, the light intensity is 55μmol / (m 2 .S), the light-to-dark time ratio was 14:10, the air flow rate was 0.6vvm, and cultured for 8 days; during the whole culture process, the salinity was controlled to be 25 by feeding sodium chloride aqueous solution with a concentration of 3mol / L;

[0040] Preparation of algae ...

Embodiment 3

[0042] The preparation method of algae powder, it comprises the steps:

[0043] Preparation of Heterococcus culture medium: improve the f / 2 medium, specifically as follows: add ammonium bicarbonate and brassinosteroid to the f / 2 medium, and control the concentrations of ammonium bicarbonate and brassinosteroid to be 40mg respectively / L and 0.025mg / L;

[0044] High-density culture: Select vigorously growing and non-polluting Heterococcus and insert them into the circular raceway pool containing Heterococcus culture solution, the inoculation density is 5 million / ml, the control temperature is 25°C, red light irradiation, red light Electromagnetic radiation having a wavelength between 730 nm and a light intensity of 50 μmol / (m 2 .S), the light-to-dark time ratio was 10:14, the air flow rate was 0.5vvm, and cultured for 7 days; during the whole culture process, the salinity was controlled to be 23 by feeding sodium chloride aqueous solution with a concentration of 2mol / L;

[0045...

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Abstract

The invention belongs to the technical field of algae, and discloses a preparation method of heterogloea powder. The preparation method comprises the following steps of step 1) preparing a heterogloeaculture solution; step 2) performing high-density culturing; and step 3) preparing algae powder. According to the method disclosed by the invention, the algae cells are precipitated through flocculation, and then the algae powder is prepared through freeze drying and crushing, so that the heterogloea powder can be applied to aquaculture, and the market application value is extremely high.

Description

technical field [0001] The invention belongs to the technical field of algae, and in particular relates to a preparation method and application of algae powder. Background technique [0002] Microalgae are rich in nutrients, and many algae contain high protein, vitamins and trace elements necessary to maintain the growth of aquatic product seedlings, which can obviously promote various physiological functions of seedlings. For example, vitamin E can promote the phagocytosis of aquatic animal macrophages, improve the activity of complement, and enhance the immunity of aquatic animal larvae; adding anabaena powder to shrimp compound feed can significantly improve the growth rate and disease resistance of shrimp. Therefore, fine algal species such as Anabaena and Chlorella have good application prospects in the cultivation of fish and shrimp fry. Adding a certain proportion of unicellular algae to the compound feed as the bait in the early stage of fish and shrimp nursery, the...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02A23K50/80A23K10/16C12R1/89
CPCC12N1/12C12N1/02A23K50/80A23K10/16Y02A40/81
Inventor 童文连鲍君美张杨康
Owner 杨凌海思生物科技有限公司
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