39results about How to "Promote cell division" patented technology

The present invention discloses an immunotherapeutic method for treating patients suffering from malignant melanoma, by administering expanded tumour-reactive CD4+ helper and / or CD8+ T-lymphocytes obtainable from one or more sentinel or metinel lymph nodes draining a malignant melanoma or a metastasis arising from malignant melanoma. The present invention provides a new effective method for treating malignant melanoma and metastatic malignant melanoma, without adverse side effects associated with the known treatments. The method comprises identification of in a patient one or more sentinel and / or metinel lymph nodes draining a malignant melanoma or a metastasis there from, resection of the one or more nodes and, optionally all or part of the tumour or metastasis, isolation of tumour-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said tumour-reactive T-lymphocytes, and administration of the thus obtained tumour-reactive T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+ helper and / or CD8+ T-lymphocytes.

The invention discloses a fruit anti-cracking nutritional agent and a preparation method thereof. The fruit anti-cracking nutritional agent comprises the following raw materials in part by weight: 5 to 15 parts of sodium carboxymethylcellulose, 3 to 7 parts of calcium, 1 to 5 parts of ethylene glycol, 0.01 to 0.9 part of plant growth regulator and 10 to 20 parts of water. The preparation method comprises the following steps of: (1) dissolving the sodium carboxymethylcellulose with the water so as to obtain sodium carboxymethylcellulose aqueous solution; (2) adding the calcium into the sodium carboxymethylcellulose aqueous solution and heating to the temperature of between 40 and 50 DEG C; and (3) adding the ethylene glycol into the solution obtained in the step (2), stirring at the temperature of between 40 and 50 DEG C for 30 to 40 minutes, lowering the temperature to the normal temperature, adding the plant growth regulator and stirring uniformly so as to obtain the fruit anti-cracking nutritional agent. The medicinal agent forms a film after being applied by spraying, is difficultly dissolved in the water for the second time after being dried, has a controllable thickness and can effectively lower fruit cracking rate by about 10 percent.

The present invention relates to a combination therapy method for treating gastrin-dependent tumors. The method comprises the immunization of a patient with an anti-gastrin 17 immunogenic composition in combination with the administration of chemotherapeutic agents such as 5-fluorouracil and leucovorin.

The invention relates to a licorice-containing skin moistening liquid soap. The liquid soap comprises, by weight, 150 parts of olive oil, 140 parts of coconut oil, 75 parts of sweet almond oil, 75 parts of castor oil, 60 parts of sunflower oil, 93 parts of potassium hydroxide, 279 parts of purified water, 20 parts of honey and 300 parts of raw licorice. Honey and licorice adopted in the invention have skin moistening and beauty treating efficacies, the above purely natural plant oils are selected, and traditional low temperature saponification is adopted, so all nutritional components of all the oils are completely reserved; and effective components extracted from honey and licorice are added to a pasty soup in order to simultaneously realize cleaning and skin moistening functions.

There are provided compositions and methods for modulating stem cell division decisions, in particular, division symmetry. It has been demonstrated that wnt7a acts through frizzled-7 receptor expressed on the surface of adult stem cells, e.g. satellite stem cells, to activate the planar cell polarity (PCP) pathway, thereby promoting symmetrical expansion of stem cells. The compositions and methods of the invention are useful, for example, in modulating stem cell division symmetry in vitro and in vivo, in replenishing and expanding the stem cell pool, and in promoting the formation, maintenance, repair and regeneration of tissue.

The present invention discloses an immunotherapeutic method for treating a patient suffering from urinary bladder cancer by administering expanded tumour-reactive CD4+ helper and / or CD8+ T-lymphocytes obtainable from one or more sentinel or metinel lymph nodes draining a tumour in the bladder or a metastasis arising from a tumour in the bladder The method provides a new effective method for treating urinary bladder cancer and metastatic bladder cancer, without adverse side effects associated with the known treatments. The method comprises identification of in a patient one or more sentinel and / or metinel lymph nodes draining a tumour in the bladder or a metastasis from a tumour in the bladder, resection of the one or more nodes and, optionally all or part of the tumour or metastasis, isolation of tumour-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said tumour-reactive T-lymphocytes, and administration of the thus obtained tumour-reactive T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+helper and / or CD8+ T-lymphocytes.

The present invention discloses an immunotherapeutic method for treating a patient suffering from a disseminated cancer by administering expanded tumour-reactive CD4+ helper and / or CD8+ T-lymphocytes obtainable from one or more metastasis-draining lymph nodes (metinel nodes) draining a metastasis. The method comprises identification of one or more metinel lymph nodes in a patient, resection of the one or more nodes and, optionally all or part of the metastases, isolation of metastasis-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said metastasis-reactive T-lymphocytes, and administration of the thus obtained T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+ helper and / or CD8+ T-lymphocytes.

The present invention discloses an immunotherapeutic method for treating a patient suffering from a disseminated cancer by administering expanded tumour-reactive CD4+ helper and / or CD8+ T-lymphocytes obtainable from one or more metastasis-draining lymph nodes (metinel nodes) draining a metastasis. The method comprises identification of one or more metinel lymph nodes in a patient, resection of the one or more nodes and, optionally all or part of the metastases, isolation of metastasis-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said metastasis-reactive T-lymphocytes, and administration of the thus obtained T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+ helper and / or CD8+ T-lymphocytes.

The invention discloses a freckle-removing and inflammation-diminishing sunscreen cream and a preparation method thereof. The formula of the freckle-removing and inflammation-diminishing sunscreen cream comprises such materials as shea butter, olive oil, coconut oil, general self-emulsifying compound emulsifier AC-402, all-powerful simple emulsifier G57, natural emulsifier GOC, emulsifier DOE, sodium acrylate SIMULGELEG, methyl silicone oil, mycose, glycerin, 25% triethanolamine solution, titanium dioxide, pearl powder, silkworm larva extract, safflower oil, cell activator, water-soluble vitamin E, polyvidone, distilled water, essence and efficient preservative Glydant. Compared with the prior art, the freckle-removing and inflammation-diminishing sunscreen cream is mild in nature and high in safety; the natural animal medicine silkworm larva is combined with other active ingredients to achieve the effects of whitening, blocking the sun, removing the pigment and keeping the skin elasticity; the coconut oil has anti-inflammation and anti-bacterial functions so that wounds can be healed quickly without inflammation or scar. The components of the formula supplement each other to effectively block the sun, realize after-sun repair, and whiten and nourish the skin, and therefore, the effects of blocking the sun, diminishing inflammation, removing freckles, maintaining beauty and eliminating the pigment can be achieved; as a result, the skin can be comprehensively improved from inside to outside.

The present invention relates to a combination therapy method for treating gastrin-dependent tumors. The method comprises the immunization of a patient with an anti-gastrin 17 immunogenic composition in combination with the administration of chemotherapeutic agents such as 5-fluorouracil and leucovorin.

The present invention discloses an immunotherapeutic method for treating patients suffering from malignant melanoma, by administering expanded tumour-reactive CD4+ helper and / or CD8+ T-lymphocytes obtainable from one or more sentinel or metinel lymph nodes draining a malignant melanoma or a metastasis arising from malignant melanoma. The present invention provides a new effective method for treating malignant melanoma and metastatic malignant melanoma, without adverse side effects associated with the known treatments. The method comprises identification of in a patient one or more sentinel and / or metinel lymph nodes draining a malignant melanoma or a metastasis there from, resection of the one or more nodes and, optionally all or part of the tumour or metastasis, isolation of tumour-reactive T-lymphocytes from said lymph nodes, in vitro expansion of said tumour-reactive T-lymphocytes, and administration of the thus obtained tumour-reactive T-lymphocytes to the patient, wherein the T-lymphocytes are CD4+ helper and / or CD8+ T-lymphocytes.

Relating to the technical field of aquaculture, the invention provides a water quality improver for aquaculture. The water quality improver comprises the following raw materials by weight: 8-15 partsof straw, 4-8 parts of lignin, 3-6 parts of sodium humate, 10-15 parts of polyacrylamide, 6-13 parts of chitosan powder, 4-9 parts of gram-positive actinomycetes, 4-9 parts of photosynthetic bacteria,2-6 parts of bacillus, 5-10 parts of lactic acid bacteria, 5-10 parts of bacillus natto, 7-14 parts of saccharomycetes and 6-18 parts of a Chinese herbal medicine additive. The water quality improvercan effectively improve the water quality of water in aquaculture, promote propagation of aquatic organisms and increase the survival rate, and meanwhile, the preparation method of the water qualityimprover is simple and easy to master, thus facilitating promotion.

The invention relates to a magnetization compound microorganism organic water solution fertilizer as well as a preparation method thereof. The magnetization compound microorganism organic water solution fertilizer is prepared from the following components in parts by weight: 3-7 parts of a CM microbial agent, 7-13 parts of active organic matter, 63-81 parts of nutrition elements, 1.4-2.6 parts of vegetable kinetin, and 7-15 parts of magnetization magnetic carrier. According to the technical scheme provided by the invention, after the application of the fertilizer, crops have excellent stress resistance, and the yield increasing effect is obvious; the fertilizer provided by the invention is added into a soil body, so that the fertilizer has the function of repairing the heavy metal polluted soil; the fertilizer is in the dosage form such asliquid or solid, the application method is simple, convenient and practical, and the fertilizer can be in spreading, hole fertilization, and furrow application, and can further be in washing fertilization and drip irrigation, so as to be suitable for mechanical operation.

The invention discloses a rooting agent for raising seedlings of bamboo willows by cutting, and belongs to the technical field of forestry and biology. Every 1000mL of the rooting agent comprises the following raw materials by weight: 50-100mg of an indolebutyric acid, 20-50mg of alpha-sodium naphthalene acetate, 50-100mg of ABT No. 2 rooting powder, 20-50mg of 6-benzyladenine, 2000-3000mg of potassium permanganate, 100-150mL of ethanol of 95%, and water is diluted to 1000mL. The rooting agent is used for directly soaking the bamboo willows, and a treatment manner of semi-lignifying a twig cutting base is applied to cutting, propagation and seedling culture of twigs of the bamboo willows. The rooting agent is used for soaking the twigs of the bamboo willows, then cutting is performed on the soaked twigs, the rooting rate reaches more than 92%, and the survival rate by cutting is obviously increased. The rooting agent disclosed by the invention has the advantages that the raw materials are easy to purchase, the cost is low, the operation is convenient, the dosage is little, the rooting agent takes effect quickly, the effect is good, the rooting agent is non-toxic and harmless, and the rooting agent acts obviously, so that the rooting agent can be extensively popularized and applied.

The invention discloses a method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds. Hemerocallis hybrida rhizome buds serve as explant materials and are directly induced to generate the adventitious buds, the adventitious buds are split into single plants and subjected to multiplication culture to obtain cluster buds, the cluster buds are split into single plants, the adventitious buds, calluses and redundant basal discs are removed, rooting culture is implemented to obtain rooting seedlings, and seedling hardening and transplanting are implemented to obtainthe hemerocallis hybrida regeneration plants. The method is simple to operate, inducing and breeding time is shortened, induction rate is high, inducing time is short, rooting rate is high, transplanting survival rate is high, the acquired regeneration plants can stably keep excellent characters of female parents, the method overcomes the shortcoming variation of future generation plants by a callus pathway, direct regeneration of tissue culture seedlings of hemerocallis hybrida is achieved, and the method serves as an effective way of industrialized production of the hemerocallis hybrida.

The invention discloses a seedling raising method of Chinese water-chestnuts, and belongs to the technical field of agricultural planting. The planting method comprises the following steps: pretreatment of seedling Chinese water-chestnuts, induction culture, multiplication culture, rooting culture, preparation of nursery gardens and transplantation, wherein in processes of induction culture, multiplication culture and rooting culture, tissue culturing is carried out through an MS culture medium and a variety of additives; in a preparation process of the nursery gardens, seedling raising fertilizer prepared from the following raw materials: well-composted farmyard fertilizer, a humic acid, soybean meal, residues of edible fungi, microorganism bacteria, poria cocos, radix notoginseng, auricularia auricula, fructus viticis negundo, firmiana flower, calcium superphosphate powder and lime powder. According to the seedling raising method of the Chinese water-chestnuts disclosed by the invention, the problems that growth advantage of tissue culture seedling obtained through a conventional tissue culturing method is not obvious, and disease resistance ability of the finally-obtained seedlings is weaker are solved.

The invention provides a soil remediation method. The method solves the problems of high costs and complicated processes of soil remediation in the prior art. The soil remediation method mainly comprises the following steps of S1, seedling cultivation, wherein a soil conditioner containing microorganisms is diluted with water by 100-200 times, a seedbed is used at 50-70 ml / m<2>, and the soil conditioner and the seedbed are all applied to soil; S2, field planting, wherein the soil conditioner diluted in step S1 is used for soil irrigation according to a standard of not less than 1000 ml / mu, andis sufficiently diluted according to the actual use amount of the soil conditioner after the field planting; S3, watering, wherein the amount of the soil conditioner diluted in step S1 is 500-700 ml / mu each watering, and the soil conditioner is flush-applied with an irrigation system.

The invention relates to a saline-alkali soil improving agent and its preparation method. The preparation method comprises the following steps: mixing Rhodopseudomonas palustris, Pichia membranefaciens Hansen bacterium, lactobacillus casei, Staestomyces microflavus and seaweed slurry, organically combining a plurality of beneficial microorganisms and seaweed through multi-time fermentation, culturing in a compound mode to obtain a novel microorganism agent-seaweed bio-fungus. adding a proper amount of humic acid, organic matter, active zeolite powder and molasses, and fermenting and drying the fermented product. The biology saline-alkali soil improving agent uses a plurality of microorganisms and employs strong effects of a unique ionic adsorbent, an organic neutralization balancing agent, an ion-exchange agent and a beneficial bacteria conversion starter to achieve the purposes of improving the soil environment, regulating acid-base balance, reducing the salt content, effectively raising the soil fertility and permeability, loosening the soil, eliminating the soil hardening, degrading the harmful substance and promoting the accumulation and conversion of the organic substance.

The invention belongs to the field of agricultural planting and particularly relates to a method for increasing the germination rate of radish seeds. The method includes the following steps that 1, the radish seeds are disinfected under ultraviolet light for 5-10 min; 2, the seeds disinfected in the step 1 are put in electrolytic saltwater to be soaked for 30-40 min and then fished out; 3, the seeds fished out in the step 2 are put in clean water to be soaked for 10-12 h and then fished out, and the seeds are put in ultrasonic waves for ultrasonic treatment for 3-5 min; 4, the seeds obtained in the step 3 are put in liquid medicine to be soaked for 2-4 h; 5, after the seeds obtained in the step 4 are fished out, the seeds are washed with clean water 2-4 times, and the seeds are subjected to germination accelerating at the temperature of 25-30 DEG C. By means of the method, the germination rate of the radish seeds is greatly increased, the germination period can be greatly shortened, and the growth period of radishes can be obviously shortened.

The invention discloses a multi-functional intelligent germination accelerating device for rice seeds. The device is composed of a box body, a power supply box, a temperature control instrument, a heating pipe, a temperature probe, a humidity control instrument, a humidity probe, a time relay, a water pump, an ultrasonic humidifier, an ozonator, a heat dissipating fan, a heat dissipating fan powersupply switch, an ozonator power supply switch, a water pipe, a spraying head, an air pipe, an air outlet head and a seed breeding disc. By using the device, the whole seed soaking and germination accelerating process of rice seeds can be completed at one time, and physiological requirements required by the seed soaking and germination accelerating of the rice seeds are met. The bottlenecks thattraditional artificial germination accelerating is limited by environmental conditions, seed soaking time is long, the germination accelerating technology is mastered difficultly and inaccurately, budgrowth is weak, buds are not orderly and not stronger, heat damage and molding phenomena occur and the like are broken through, and better conditions are created for stronger seedling growth. The device is beneficial for rice seed industrialization, standardization, large-scale seedling and intensification seedling growing.

The invention discloses a method for promoting maturation of ottelia acuminate seeds. The method includes the step that hardened and incompletely-mature ottelia acuminate seeds with white seed coats are adopted and cultured in first accelerant liquid and second accelerant liquid till the seeds are completely mature. The defect that mature ottelia-acuminate seeds are difficult to collect is overcome. As ZT and IAA in the first accelerant liquid have the effect of promoting cell division of immature seeds, and DMSO has quite high permeability, the ZT and the IAA can permeate into cells and directly act on embryos and albuminous cells, and the embryos and endosperm of the immature seeds are perfectly developed. As GA3 and 6-BA in the second accelerant liquid have the effect of promoting cell division, the seeds can be mature; meanwhile, the GA3 and the 6-BA in the second accelerant liquid have the effects of promoting conversion of starch in the endosperm into soluble saccharide and seed germination. The method is easy to operate and low in production cost; a large number of mature ottelia acuminate seeds can be obtained in a short time, mass seedling breeding is carried out, and high quality seedlings are provided for artificial cultivation of ottelia acuminate.

The invention mainly relates to the technical field of hybrid planting of traditional Chinese medicines, and discloses a low-altitude hybrid planting method for eucommia ulmoides and polygonatum kingianum. The low-altitude hybrid planting method for eucommia ulmoides and polygonatum kingianum comprises the steps of soil preparation, eucommia ulmoides planting, eucommia ulmoides management, polygonatum kingianum planting, polygonatum kingianum management and harvesting treatment. The method is simple, a large amount of soil, labors and materials are saved, growth of the eucommia ulmoides and the polygonatum kingianum is promoted, the eucommia ulmoides can be peeled off 7-8 years after being planted, the yield can reach 627 kg / mu, the polygonatum kingianum reproduced by rhizomes can be harvested 1 year after being planted, the polygonatum kingianum reproduced by seeds can be harvested 2 years after being planted, the yield reaches 583 kg / mu, a new method is provided for planting of the eucommia ulmoides and the polygonatum kingianum in Anhui area, and the planting cost is saved by 39.4%; and the eucommia ulmoides and the polygonatum kingianum are planted in a low-altitude region, theeucommia ulmoides and the polygonatum kingianum which adapt to a low-altitude environment can be gradually selected out, the planting difficulty is reduced, and the planting cost is saved.

The invention belongs to the technical field of plant toners, and particularly relates to a plant toner and an application and use method thereof. The plant toner is prepared through the steps of mixing lactobacillus rhamnosus with various minerals to obtain a mixture, adding the mixture to culture media, and performing fermentation under certain condition for definite time to obtain the plant toner. The plant toner comprises the following components in parts by weight of 7-9 parts of lactobacillus rhamnosus, 1-2 parts of calcium, 0.25 part of magnesium, 0.1 part of boron, 0.5 part of potassium and 0.15 part of ferrum. While in use, the toner is diluted with water until the diluted toner is 800-1000 times of the toner which is not diluted, and at different stages of plant growth, the diluted toner is sprayed onto surfaces of plants. The plant toner disclosed by the invention has the advantages that product components only contain microorganisms and mineral elements, and exclude chemical pesticides, chemical compounds, plant hormones and the like, so that security and ecological security of agriculture products are guaranteed, and besides, the effect of increasing color for fruits can be achieved.

The invention relates to a preparation method and application of an immune preparation capable of enhancing immunities of fishes, prawns and crabs, and belongs to the technical field of nutrition immunology. By feeding an immuno-potentiator containing such nutrients as nitric oxide, amino acid, beta-brown shell color, anthocyanin, vitamins and trace elements, oxygen radicals and viruses in the fishes, prawns and crabs are removed or inhibited; moreover, the immune preparation can regulate metabolism of bred animals, and has a certain growth promoting effect; the immune preparation is favorable for reducing a death rate and improving breed output of the bred animals.

The invention relates to nutritive and instant Chinese yam paste food. The food is the brewing powder instant food which can be eaten by ripening each component raw material and adding boiled water; and the food is prepared from, by weight, 500-600 parts of Chinese yam, 100-150 parts of black soya beans, 100-120 parts of polished round-grained rice, 50-100 parts of black sesame, 20-50 parts of barbary wolfberry fruits, 10-35 parts of finger citron, 10-30 parts of kudzuvine root and 3-30 parts of rock sugars. The food takes the Chinese yam as a basis and adds the kudzuvine root, the finger citron, the barbary wolfberry fruits, the black soya beans, small red beans, the black sesame and some medicine and food homologous food, so that the food can be eaten as satiety food and has health careeffects; the food is abundant in nutrition and has a certain of health care functions; the food is scientific in proportioning and balanced in nutrition; without adding pigments and sugar and frying,the original nutrition of components can be well reserved, so that the food can be conveniently absorbed by human bodies, and tastes can be improved; and the food is convenient for eating, so that thefood is worth being widely popularized.