Magnetization compound microorganism organic water solution fertilizer as well as preparation method and application thereof
A compound microorganism and fertilizer technology, which is applied in the field of magnetized compound microorganism organic water-soluble fertilizer and its preparation, achieves the effects of easy absorption and utilization, quick effect, and simple and easy preparation and use methods
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Embodiment 1
[0042] The preparation (1) of embodiment 1 magnetization composite microbial organic water-soluble fertilizer
[0043] 1. Preparation of CM bacterial agent:
[0044] ① Preparation of Vesical-Arbuscular and Root of yeast bacteria:
[0045] Take a root about 4 cm long from the root tip of a fresh plant, wash the soil on the root with clean water, and then treat it with 75% ethanol for 20 seconds, and then use the newly prepared 0.1% mercuric chloride solution (mercuric chloride 1g, concentrated hydrochloric acid 2.5mL) , distilled water 1L) for 5 minutes, rinse with sterile water 5 times. Use a sterile scalpel to cut the root into 3 mm thin slices in a sterile petri dish, place the thin slices in a petri dish filled with PDA medium with sterile forceps, and put 3 pieces in each dish. Cultured in a 25°C constant temperature incubator protected from light. Observe frequently, when the mycelium grows from the tissue block to form a colony of a certain size, pick the mycelium and...
Embodiment 2
[0055] The preparation (2) of embodiment 2 magnetization compound microbial organic water-soluble fertilizers
[0056] 1. Preparation of CM bacterial agent:
[0057] ① Preparation of Vesical-Arbuscular and Root of yeast bacteria:
[0058] Take a root about 3 cm long from the root tip of a fresh plant, wash the soil on the root with clean water, and then treat it with 75% ethanol for 30 seconds, and then use the newly prepared 0.1% mercuric chloride solution (mercuric chloride 1g, concentrated hydrochloric acid 2.5mL) , distilled water 1L) for 10 minutes, rinse with sterile water 5 times. Use a sterile scalpel to cut the roots into 5mm thin slices in a sterile petri dish, place the thin slices in a petri dish filled with PDA medium with sterile tweezers, and put 4 pieces in each dish. Cultured in a 25°C constant temperature incubator protected from light. Observe frequently, when the mycelium grows from the tissue block to form a colony of a certain size, pick the mycelium a...
Embodiment 3
[0068] The preparation (3) of embodiment 3 magnetization compound microbial organic water-soluble fertilizers
[0069] 1. Preparation of CM bacterial agent:
[0070] ① Preparation of Vesical-Arbuscular and Root of yeast bacteria:
[0071] Take a root about 4 cm long from the root tip of a fresh plant, wash the soil on the root with clean water, and then treat it with 75% ethanol for 25 seconds, and then use the newly prepared 0.1% mercuric chloride solution (mercuric chloride 1g, concentrated hydrochloric acid 2.5mL) , distilled water 1L) for 8 minutes, rinse with sterile water 5 times. Use a sterile scalpel to cut the roots into 4mm thin slices in a sterile petri dish, place the thin slices in a petri dish filled with PDA medium with sterile forceps, and put 4 pieces in each dish. Cultured in a 25°C constant temperature incubator protected from light. Observe frequently, when the mycelium grows from the tissue block to form a colony of a certain size, pick the mycelium and...
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