Seedling raising method of Chinese water-chestnuts
A technology of horseshoe and tissue cultured seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of weak disease resistance of seedlings, unobvious growth advantages of tissue cultured seedlings, etc., and reduce the production of toxic substances , solve the seedling degeneration, promote the effect of cell division
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[0024] Example 1
[0025] A method for raising seedlings of horseshoes includes the following steps:
[0026] (1) Seed water chestnut pretreatment: first, select the abundant, disease-free horseshoe as the seed water chestnut; then, the seed water chestnut is cleaned, sterilized, and germinated; then, the main and lateral buds of the seed water chestnut are cut as Explants; finally, the explants are cleaned and disinfected for later use. The method for disinfecting the explants includes the following steps: firstly, treating the explants with 75% ethanol for 30 seconds; then, disinfecting the explants with 0.1% mercury for 10 minutes; finally, removing the explants with The bacteria water was washed 5 times, and the sterile water on the explant was blotted dry with sterile paper.
[0027] (2) Induction culture: inoculate the explants sterilized in step (1) into an induction medium. The induction medium used MS medium as the basic medium, and added the following mass concentrations...
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[0035] Example 2
[0036] A method for raising seedlings of horseshoe includes the following steps:
[0037] (1) Seed water chestnut pretreatment: first, select the abundant, disease-free horseshoe as the seed water chestnut; then, the seed water chestnut is washed, sterilized, and germinated; then, the main and lateral buds of the seed water chestnut are cut as Explants; finally, the explants are cleaned and disinfected for later use. The method for disinfecting the explants includes the following steps: firstly, treating the explants with 75% ethanol for 60 seconds; then, disinfecting the explants with 0.1% mercury for 12 minutes; finally, removing the explants with The bacteria water was washed 5-6 times, and the sterile water on the explant was blotted dry with sterile paper.
[0038] (2) Induction culture: inoculate the explants sterilized in step (1) into an induction medium. The induction medium used MS medium as the basic medium, and added the following mass concentrations...
Example Embodiment
[0046] Example 3
[0047] A method for raising seedlings of horseshoe includes the following steps:
[0048] (1) Seed water chestnut pretreatment: first, select the abundant, disease-free horseshoe as the seed water chestnut; then, the seed water chestnut is washed, sterilized, and germinated; then, the main and lateral buds of the seed water chestnut are cut as Explants; finally, the explants are cleaned and disinfected for later use. The method for disinfecting the explants includes the following steps: first, treating the explants with 75% ethanol for 45s; then, disinfecting the explants with 0.1% mercury for 11 minutes; finally, using the explants with no The bacteria water was washed 6 times, and the sterile water on the explant was blotted dry with sterile paper.
[0049] (2) Induction culture: inoculate the explants sterilized in step (1) into an induction medium. The induction medium uses MS medium as the basic medium, and adds the following mass concentrations of additive...
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