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Seedling raising method of Chinese water-chestnuts

A technology of horseshoe and tissue cultured seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of weak disease resistance of seedlings, unobvious growth advantages of tissue cultured seedlings, etc., and reduce the production of toxic substances , solve the seedling degeneration, promote the effect of cell division

Inactive Publication Date: 2018-01-30
博白县富山水果种植专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a horseshoe seedling raising method to solve the problem that the growth advantage of the tissue cultured seedlings obtained by traditional tissue culture is not obvious, and the finally obtained seedlings have weak disease resistance

Method used

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  • Seedling raising method of Chinese water-chestnuts
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Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for raising seedlings of horseshoe, comprising the following steps:

[0026] (1) Pretreatment of chestnuts: first, select plump, disease-free horseshoes as chestnuts; then, wash, sterilize, and accelerate germination of the chestnuts; then, cut the main and lateral buds of the chestnuts as Explants; finally, the explants are cleaned and disinfected for use. The method for disinfecting the explants comprises the following steps: first, treating the explants with 75% ethanol for 30s; then, disinfecting the explants with 0.1% mercuric chloride for 10 minutes; Bacterial water was washed 5 times, and the sterile water on the explant was blotted dry with sterile paper.

[0027](2) Induction culture: inoculate the explants sterilized in step (1) into induction medium. The induction medium uses MS medium as the basic medium, and adds the following additives at the mass concentration: 6-benzylaminoadenine 2mg / L, naphthaleneacetic acid 0.1mg / L, dichlorophenoxyacetic ac...

Embodiment 2

[0036] A method for raising seedlings of horseshoe, comprising the following steps:

[0037] (1) Pretreatment of chestnuts: first, select plump, disease-free horseshoes as chestnuts; then, wash, sterilize, and accelerate germination of the chestnuts; then, cut the main and lateral buds of the chestnuts as Explants; finally, the explants are cleaned and disinfected for use. The method for disinfecting the explants comprises the following steps: first, treating the explants with 75% ethanol for 60 seconds; then, disinfecting the explants with 0.1% mercuric chloride for 12 minutes; Bacterial water was washed 5-6 times, and the sterile water on the explant was blotted dry with sterile paper.

[0038] (2) Induction culture: inoculate the explants sterilized in step (1) into induction medium. The induction medium uses MS medium as the basic medium, and adds the following additives at the mass concentration: 6-benzylaminoadenine 3mg / L, naphthaleneacetic acid 0.2mg / L, dichlorophenox...

Embodiment 3

[0047] A method for raising seedlings of horseshoe, comprising the following steps:

[0048] (1) Pretreatment of chestnuts: first, select plump, disease-free horseshoes as chestnuts; then, wash, sterilize, and accelerate germination of the chestnuts; then, cut the main and lateral buds of the chestnuts as Explants; finally, the explants are cleaned and disinfected for use. The method for disinfecting the explants comprises the following steps: first, treating the explants with 75% ethanol for 45 seconds; then, disinfecting the explants with 0.1% mercuric chloride for 11 minutes; Bacterial water was washed 6 times, and the sterile water on the explant was blotted dry with sterile paper.

[0049] (2) Induction culture: inoculate the explants sterilized in step (1) into induction medium. The induction medium uses MS medium as the basic medium, and additional additives with the following mass concentrations: 6-benzylaminoadenine 2.5mg / L, naphthaleneacetic acid 0.15mg / L, dichloro...

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Abstract

The invention discloses a seedling raising method of Chinese water-chestnuts, and belongs to the technical field of agricultural planting. The planting method comprises the following steps: pretreatment of seedling Chinese water-chestnuts, induction culture, multiplication culture, rooting culture, preparation of nursery gardens and transplantation, wherein in processes of induction culture, multiplication culture and rooting culture, tissue culturing is carried out through an MS culture medium and a variety of additives; in a preparation process of the nursery gardens, seedling raising fertilizer prepared from the following raw materials: well-composted farmyard fertilizer, a humic acid, soybean meal, residues of edible fungi, microorganism bacteria, poria cocos, radix notoginseng, auricularia auricula, fructus viticis negundo, firmiana flower, calcium superphosphate powder and lime powder. According to the seedling raising method of the Chinese water-chestnuts disclosed by the invention, the problems that growth advantage of tissue culture seedling obtained through a conventional tissue culturing method is not obvious, and disease resistance ability of the finally-obtained seedlings is weaker are solved.

Description

technical field [0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for raising seedlings of horseshoes. Background technique [0002] Horseshoe, also known as water chestnut, belongs to the monocot Cyperaceae, and uses underground bulbs as fruits and vegetables for people to eat. The skin of the horseshoe is black and bright, and the inside is pure white. It tastes delicious and has the effect of melting food. It is called "Underground Sydney" because of its nutritional value. Horseshoe is rich in various minerals, vitamins, and amino acids needed by the human body. It has the functions of clearing heat and detoxification, diuresis, and lowering blood pressure. It is an excellent fruit and vegetable food with both medicine and food, and it is also one of the important agricultural products exported by my country. At present, horseshoe is popular among consumers in the international market. Its main products in...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 刘怡玲
Owner 博白县富山水果种植专业合作社
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