Method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds

A technology of Hemerocallis grandiflora and regenerated plants, which is applied in the field of plant tissue culture, to achieve the effects of facilitating rooting, preventing browning, and increasing the content of

Inactive Publication Date: 2019-05-07
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the number of chromosomes, DNA content, and the proportion of DNA polymorphic fragments will vary to varying degrees with the increase in the number of s

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  • Method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds

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Example Embodiment

[0039] Example 1 The method for inducing adventitious buds to obtain regeneration plants of Hemerocallis grandiflora includes the following steps:

[0040] 1) Select explants, pretreatment

[0041] Select the 1-2 cm long, unearthed rhizome sprouts of Hemerocallis grandiflora as explants, wash the soil, cut off all the roots, peel off the outermost 2-3 layers of leaves, wash and soak them with detergent Rinse under running water for about 20 minutes for 2 to 3 hours. On the ultra-clean workbench, first treat the explants with 75% alcohol for 30 seconds, rinse with sterile water 3 times, and wipe dry with sterile filter paper; then sterilize with 0.1% mercury liter solution for 10-12 minutes, and immerse in sterile water Wash 5 times, 1-2 minutes each time; then use sterile filter paper to absorb the water on the surface of the explant, cut off the outermost tissue of the base, and set aside.

[0042] 2) Induce adventitious buds

[0043] Divide the pretreated explants into 3 small pie...

Example Embodiment

[0056] Embodiment 2 The method of breeding Hemerocallis grandiflora in factory includes the following steps:

[0057] 1) Select explants, pretreatment

[0058] Select the 1-2 cm long, unearthed rhizome shoots of Hemerocallis grandiflora as explants, wash the soil, cut off all the roots, peel off the outermost 2-3 layers of leaves, wash and soak with detergent for 20 minutes Rinse under running water for 2 to 3 hours. On the ultra-clean workbench, first treat the explants with 75% alcohol for 30 seconds, rinse with sterile water 3 times, and wipe dry with sterile filter paper; then sterilize with 0.1% mercury liter solution for 10-12 minutes, and immerse in sterile water Wash 5 times, 1-2 minutes each time; then use sterile filter paper to absorb the water on the surface of the explant, cut off the outermost tissue of the base, and set aside.

[0059] 2) Induce adventitious buds

[0060] Divide the pretreated explants into 2 small pieces, inoculate them on the induction medium and in...

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Abstract

The invention discloses a method for acquiring hemerocallis hybrida regeneration plants by inducing adventitious buds. Hemerocallis hybrida rhizome buds serve as explant materials and are directly induced to generate the adventitious buds, the adventitious buds are split into single plants and subjected to multiplication culture to obtain cluster buds, the cluster buds are split into single plants, the adventitious buds, calluses and redundant basal discs are removed, rooting culture is implemented to obtain rooting seedlings, and seedling hardening and transplanting are implemented to obtainthe hemerocallis hybrida regeneration plants. The method is simple to operate, inducing and breeding time is shortened, induction rate is high, inducing time is short, rooting rate is high, transplanting survival rate is high, the acquired regeneration plants can stably keep excellent characters of female parents, the method overcomes the shortcoming variation of future generation plants by a callus pathway, direct regeneration of tissue culture seedlings of hemerocallis hybrida is achieved, and the method serves as an effective way of industrialized production of the hemerocallis hybrida.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for obtaining regenerated plants of Hemerocallis grandiflorum by inducing adventitious buds. Background technique [0002] Hemerocallis (Hemerocallis), also known as golden needle, forget worry grass, day lily, Yiman grass, cure sorrow, etc., is a perennial herbaceous flower of the genus Hemerocallis in the family Liliaceae. It has the advantages of beautiful shape, various colors, robust growth, and wide adaptability. , flower beds, and important flowers in street greening. [0003] Hemerocallis fulva, also known as polyploid Hemerocallis fulva, is a processed polyploid dwarf variety based on Hemerocallis fulva. The propagation methods of Hemerocallis mainly include seedling method, stem bud cutting method, division method and tissue culture method. [0004] The seedlings produced by sowing are more suitable for colder climates, but the produc...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨柳燕王艺程张永春蔡友铭
Owner SHANGHAI ACAD OF AGRI SCI
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