Seedling method for tissue culture and rapid breeding of camellia semiserrata chi

Abstract

The present invention discloses a seedling method for tissue culture and rapid breeding of camellia semiserrata chi. The method comprises: explant collecting, aseptic differentiation, strong seedling culturing and bagged seedling transplanting, wherein the explant is provided for breeding. According to the method, bud tip or leaf is adopted as the explant for the breeding, and is subjected to treatments of disinfection and impurity removing; a differentiation culture media is adopted to culture the treated bud tip or the treated leaf to form a small plant; the induced small plant is cut, and inoculated in a new differentiation culture media to carry out multiple subcultures; the resulting small plant is inoculated on the specially-made strong seedling culture media to carry out culture; finally the bagged seedling transplanting is performed. According to the present invention, the original culture media component is adopted; the culture media adopts the oriented polypropylene film bag as the culture dish; the tea seedlings breed rapidly; a lot of plant viruses can be removed, such that the original fine varietal characters can be maintained; the seedling has characteristics of good quality, high transplanting survival rate, rapid flowering, rapid fruiting, high oil yield, high oil quality and long high yield period; the method of the present invention has characteristics of strong operability, high application value and originality, and provides a new approach for the camellia oleifera seedling breeding, wherein the approach has characteristics of rapid breeding and rapid commissioning.

Description

technical field [0001] The invention relates to a rare edible vegetable oil seedling breeding method, in particular to a seedling raising method for tissue culture and rapid propagation of Camellia oleifera. Background technique [0002] Camellia oleifera belongs to the Theaceae family Camellia genus. It is an evergreen small tree that blooms from December to March of the next year with large flowers and fruits. It is an excellent tree species for the production of edible oil, ornamental plants and greening of barren hills; fruit picking in autumn . [0003] Daguo safflower camellia has great economic value, and the whole tree is a treasure. Camellia oleifera fruit is composed of husk, seed shell and seed kernel, the ratio is 63.64:14.38:21.98, the weight of a single fruit is 0.5-1.5 kg, the oil yield of dry seeds is 50-62%, the leaves of the trees are large and thick, evergreen, Conducive to water conservation, protection of the ecological environment. The husk can be us...

AI Technical Summary

Problems solved by technology

[0004] Due to the wide distribution range of ordinary camellia oleifera, the fruit of camellia oleifera is small, the seed setting rate is low, the oil yield is low, and it contains more undesirable substances such as polyphenols and erucic acid; the traditional propagation method uses seedlings and grafted seedlings, and the seedlings take 2 to 3 years to bloom and bear fruit. The production is slow. It takes 10-15 years for seedlings to flower and bear fruit after planting, and it takes 6-7 years for grafted seedlings to bloom and bear fruit.