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A kind of tissue engineering artificial skin and preparation method thereof

An artificial skin and tissue engineering technology, which is applied in the field of tissue engineering biomedical materials and medical devices, can solve the problems of secondary skin grafting, mismatch of mechanical properties of the epidermis, easy separation of the epidermis and dermis, etc., and achieve stable scaffold structure, Effects that contribute to dermal regeneration and proliferation

Active Publication Date: 2017-11-24
BEIJING PAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Yongqing developed a method for preparing artificial skin (Patent Publication No. CN1485099A) to prepare an artificial skin containing epidermis and dermis, but the skin has the problem that the epidermis and dermis are easy to separate and cannot withstand surgical suturing
She Zhending developed a slow-release growth factor double-layer artificial skin (patent publication number CN101716376A), and the collagen-chitosan and silicone rubber double-layer skin regeneration scaffold developed by Gao Changyou and its preparation method (publication number CN1785444A) are also There is a mismatch between the epidermis and the mechanical properties of human skin and the need for secondary skin grafting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: Tissue engineering artificial skin comprises epidermis and dermis, and epidermis is made up of dense, semi-permeable film with microporous structure, and material is collagen, and described dermis is made up of two layers of different porosity Composed of a three-dimensional porous scaffold structure, the two layers form a two-level gradient structure due to different porosity, and the material is also collagen.

[0033] The preparation method of tissue engineering artificial skin is as follows:

[0034] Step 1: Use 1% acetic acid solution as a solvent, take a certain amount of type I collagen, prepare a collagen solution with a higher concentration, 0.6wt% solution, put it into a dialysis bag and dialyze for 7 days to form a solution I;

[0035] Step 2: Take a certain amount of type I collagen with a 1% concentration of acetic acid solution as a solvent, and configure a collagen solution with a lower concentration of 0.3wt% to form a solution II;

[003...

Embodiment 2

[0041] Embodiment 2: Tissue engineering artificial skin comprises epidermis and dermis, and epidermis is made up of dense, semi-permeable film with microporous structure, and material is collagen, and described dermis is made up of two layers of different porosity Composed of a three-dimensional porous scaffold structure, the two layers form a two-level gradient structure due to different porosity, and the material is a mixture of collagen and chondroitin sulfate.

[0042] The preparation method of tissue engineering artificial skin is as follows:

[0043] Step 1: Use 0.01% hydrochloric acid solution as a solvent, take a certain amount of type I collagen, prepare a collagen solution with a higher concentration, the solution with a concentration of 1.2wt%, put it in a dialysis bag and dialyze for 5 days to form a solution I;

[0044] Step 2: With 0.01% concentration of hydrochloric acid solution solvent, take a certain amount of type I collagen, configure a mixed solution of c...

Embodiment 3

[0050] Embodiment 3: Tissue engineering artificial skin comprises epidermis and dermis, and epidermis is made up of dense, semi-permeable film with microporous structure, and material is the blend material of collagen protein and chitosan, and described dermis The layer is composed of two layers of three-dimensional porous scaffold structure with different porosity. The two layers form a two-level gradient structure due to the difference in porosity. The material is a mixture of collagen and sodium hyaluronate.

[0051] The preparation method of tissue engineering artificial skin is as follows:

[0052] Step 1: take the acetic acid solution of 0.5% concentration as solvent, get a certain amount of type I collagen and chitosan, configure the higher collagen solution of concentration, concentration is the solution of 0.9wt%, wherein solute collagen and chitosan The weight ratio of sugar is 1:1, put it into a dialysis bag for dialysis, and form solution I for 3 days;

[0053] St...

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Abstract

The invention provides a tissue engineering artificial skin and a preparation method thereof. The artificial skin includes an epidermis and a dermis, the epidermis is made of collagen or a blend of collagen and chitosan, and the dermis is made of collagen or collagen and chitosan, hyaluronic acid, Blend material of chondroitin sulfate, alginate or polyvinyl alcohol. The epidermis consists of a semi-permeable membrane, and the cortex consists of two layers of three-dimensional porous scaffold structures with different porosities. The collagen used is antelopeptide collagen, which eliminates the problems of immune rejection, foreign body reaction and virus infection during the application of artificial skin. The invention also provides a preparation method of the tissue engineering artificial skin.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomedical materials and medical devices, and relates to a tissue engineering artificial skin and a preparation method thereof. Background technique [0002] The skin is the largest and one of the most important organs in the human body. It has the functions of breathing, water retention and biological barrier. However, skin defects caused by various reasons such as burns, infections, and friction have become one of the common diseases. How can we develop an alternative? Tissue-engineered skin of human skin has become a research hotspot. The research scope of skin defect becoming tissue engineered skin includes artificial epidermis, artificial dermis and full-thickness skin. Among them, the most respected is the artificial skin that contains both the dermis and the epidermis (quoted from: the development of human full-thickness tissue-engineered skin, Jin Yan, etc.). [0003] In order to solve ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/26A61L27/24A61L27/20A61L27/16A61L27/54A61L27/38
Inventor 符斌张自强杨冰徐荣荣
Owner BEIJING PAISHENG BIOTECH CO LTD
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