Tissue engineering artificial skin and preparation method thereof

A technology of artificial skin and tissue engineering, applied in the field of tissue engineering biomedical materials and medical devices, can solve the problems of secondary skin grafting, mismatch of mechanical properties of epidermis, easy separation of epidermis and dermis, etc. , Conducive to the regeneration of the dermis and the stability of the scaffold structure

Active Publication Date: 2015-10-21
BEIJING PAISHENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Yongqing developed a method for preparing artificial skin (Patent Publication No. CN1485099A) to prepare an artificial skin containing epidermis and dermis, but the skin has the problem that the epidermis and dermis are easy to separate and cannot withstand surgical suturing
She Zhending developed a slow-release growth factor double-layer artificial skin (patent publication number CN101716376A), and the collagen-chitosan and silicone rubber double-layer skin regeneration scaffold developed by Gao Changyou and its preparation method (publication number CN1785444A) are also There is a mismatch between the epidermis and the mechanical properties of human skin and the need for secondary skin grafting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 : Tissue engineering artificial skin comprises epidermis and dermis, epidermis is made up of compact, semi-permeable film with microporous structure, and material is collagen, and described dermis is made of three-dimensional porous of two layers of different porosity The scaffold structure is composed of two layers with different porosity to form a two-level gradient structure, and the material is also collagen.

[0033] The preparation method of tissue engineering artificial skin is as follows:

[0034] Step 1: Use 1% acetic acid solution as a solvent, take a certain amount of type I collagen, prepare a collagen solution with a higher concentration, 0.6wt% solution, put it into a dialysis bag and dialyze for 7 days to form a solution I;

[0035] Step 2: Take a certain amount of type I collagen with a 1% concentration of acetic acid solution as a solvent, and configure a collagen solution with a lower concentration of 0.3wt% to form a solution II;

[0036]...

Embodiment 2

[0041] Example 2: Tissue-engineered artificial skin includes epidermis and dermis. The epidermis is composed of a dense, semi-permeable film with a microporous structure. The material is collagen. The dermis is composed of two layers of three-dimensional porous scaffolds with different porosities. Structural composition, the two layers form a two-level gradient structure due to different porosity, and the material is a mixture of collagen and chondroitin sulfate.

[0042] The preparation method of tissue engineering artificial skin is as follows:

[0043] Step 1: Use 0.01% hydrochloric acid solution as a solvent, take a certain amount of type I collagen, prepare a collagen solution with a higher concentration, the solution with a concentration of 1.2wt%, put it in a dialysis bag and dialyze for 5 days to form a solution I;

[0044] Step 2: With 0.01% concentration of hydrochloric acid solution solvent, take a certain amount of type I collagen, configure a mixed solution of ...

Embodiment 3

[0050] Example 3 : Tissue engineering artificial skin comprises epidermis and dermis, epidermis is made up of dense, semi-permeable film with microporous structure, material is collagen and chitosan blend material, described dermis is made of two It is composed of three-dimensional porous scaffold structure with different porosity. The two layers form a two-level gradient structure because of the different porosity. The material is a mixture of collagen and sodium hyaluronate.

[0051] The preparation method of tissue engineering artificial skin is as follows:

[0052] Step 1: take the acetic acid solution of 0.5% concentration as solvent, get a certain amount of type I collagen and chitosan, configure the higher collagen solution of concentration, concentration is the solution of 0.9wt%, wherein solute collagen and chitosan The weight ratio of sugar is 1:1, put it into a dialysis bag for dialysis, and form solution I for 3 days;

[0053] Step 2: With 0.9% concentration of ...

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Abstract

The invention provides a tissue engineering artificial skin and a preparation method thereof. The artificial skin comprises an epidermal layer and a dermal layer, wherein the epidermal layer material is made of collagen or a mixed material of collagen and chitosan; the dermal layer material is made of collagen or a mixed material of collagen and chitosan, hyaluronic acid, chondroitin sulfate, alginate or polyving akohol. The epidermal layer comprises a semipermeable film; the dermal layer comprises two layers of three-dimensional porous supporting structures with different porosities. The used collagen is end-free collagen, so that the problems of immune rejection, foreign body reaction, viral infection and the like of the artificial skin during application are solved. The invention further provides the preparation method for the tissue engineering artificial skin.

Description

technical field [0001] The invention belongs to the field of tissue engineering biomedical materials and medical devices, and relates to a tissue engineering artificial skin and a preparation method thereof. Background technique [0002] The skin is the largest and one of the most important organs in the human body. It has the functions of breathing, water retention and biological barrier. However, skin defects caused by various reasons such as burns, infections, and friction have become one of the common diseases. How can we develop an alternative? Tissue-engineered skin of human skin has become a research hotspot. The research scope of skin defect becoming tissue engineered skin includes artificial epidermis, artificial dermis and full-thickness skin. Among them, the most respected is the artificial skin that contains both the dermis and the epidermis (quoted from: the development of human full-thickness tissue-engineered skin, Jin Yan, etc.). [0003] In order to solve ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/26A61L27/24A61L27/20A61L27/16A61L27/54A61L27/38
Inventor 符斌其他发明人请求不公开姓名
Owner BEIJING PAISHENG BIOTECH CO LTD
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