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A method for producing 2,3-butanediol with microalgae as raw material

A technology of butanediol and microalgae, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low amount of hydrolyzed sugar, high cost and low content of 2,3-butanediol, etc. Achieve high starch content, increase fermentable sugar content, and save energy and energy

Active Publication Date: 2020-09-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problems of low content of microalgae starch, low amount of hydrolyzed sugar and high cost of producing 2,3-butanediol from other raw materials, the present invention provides a method for producing 2,3-butanediol using microalgae as raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of microalgae seed solution: Chlorella ( Chlorella vulgaris ) seeds were inserted into BG11 microalgae culture medium, at a temperature of 30°C, an air flow of 0.25vvm, CO 2 The content is 1v%, the stirring speed is 100rpm, the pH is 7.0, and the light-dark ratio is 10:14, and it is cultivated to the logarithmic growth phase.

[0038] (1) Microalgae cultivation: insert the microalgae seed liquid into the microalgae medium rich in N element according to the inoculation amount of 5v%, and the enrichment of N element means adding NaNO to the medium at 2.5g / L 3 , cultivate until the logarithmic growth phase stops; discharge the supernatant after standing and settling, add a medium containing trace N elements to continue culturing, NaNO 3 0.05 g / L, and 30 mmol / L 3-phosphoglycerate and 50 μmol / L pine needle oil were added to the culture medium. The above-mentioned microalgae cultivation is carried out at a temperature of 30°C, an aeration rate of 0.25vvm, CO 2...

Embodiment 2

[0045] The treatment process and operating conditions were the same as those in Example 1, except that in step (1), 30 mmol / L 3-phosphoglycerate and 50 μmol / L dipropen were added to the medium at the same time. The microalgae are cultivated to the stable stage, and the algae liquid is harvested, in which the starch content reaches more than 50%. After hydrolysis treatment, the sugar concentration in the hydrolyzate is about 80g / L. After 48 hours of fermentation, the sum of the final concentrations of 2,3-butanediol and acetoin in the fermentation broth was 75.23g / L.

Embodiment 3

[0047] The treatment process and operating conditions were the same as those in Example 1, except that in step (1) 30 mmol / L 3-phosphoglycerate and 50 μmol / L benzotrione were added to the culture medium at the same time. The microalgae are cultivated to the stable stage, and the algae fluid is harvested, in which the starch content reaches more than 50%. After hydrolysis treatment, the sugar concentration in the hydrolyzate is about 80g / L. After 48 hours of fermentation, the sum of the final concentrations of 2,3-butanediol and acetoin in the fermentation broth was 75.78 g / L.

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Abstract

The invention relates to a method for producing 2,3-butanediol with microalgae as a raw material. The method comprises the following steps: (1) inoculating a microalgal medium rich in elemental N withmicroalgal seed liquid into and carrying out culturing until an logarithmic growth phase is reached, carrying out standing and settling, discharging a supernatant, adding a microalgal medium containing trace elemental N, continuing culturing, adding both an AGPase activator and an ACCase inhibitor into the medium, and carrying out culturing until a stable phase is reached so as to obtain a microalgal culture solution; (2) adding helicase into the microalgal culture solution, carrying out wall breaking, adding an inorganic strong acid to allow the final acid concentration of a hydrolysis system to be 0.5-5.0 wt%, carrying out heating to 100-150 DEG C and then conducting hydrolysis to obtain microalgal hydrolysate; (3) preparing a fermentation medium with the obtained microalgal hydrolysateas a carbon source and carrying out fermentation for production of 2,3-butanediol. The method of the invention increases the starch content of microalgae and the content of fermentable sugars in thehydrolyzate through the regulation of microalgal cultivation process and hydrolysis process, and high-concentration 2,3-BDO is finally obtained; and the method has the advantages of simple treating process, low cost, high yield and other characteristics.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for producing 2,3-butanediol by using microalgae as a raw material. Background technique [0002] Due to the depletion of world oil resources and rising prices, increasingly severe environmental issues, and a series of impacts caused by global climate change, since the 1970s, many countries have paid more and more attention to the development of bio-energy, bio-based chemicals and bio-materials. 2,3-Butanediol (2,3-BDO) is a very important chemical raw material and liquid fuel, widely used in food, medicine, building materials, fuel, aerospace and other fields. Currently, there are two methods for synthesizing 2,3-butanediol, chemical method and biological method. Chemical synthesis of 2,3-butanediol is mainly obtained by hydrolysis of four types of hydrocarbons produced during petroleum cracking under high temperature and high pressure. Compare...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/18C12R1/22
CPCC12P7/18
Inventor 廖莎王鹏翔师文静孙启梅樊亚超张霖李晓姝高大成王领民
Owner CHINA PETROLEUM & CHEM CORP
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