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Novel dual-layer nerve conduit and preparation method thereof

A nerve conduit and nerve technology, applied in the field of biomaterials and nerve repair, can solve the problems of surrounding tissue compression, lack of mechanical support, access to nerves, etc., to prevent proliferation and invasion of surrounding tissues, not easy to inflammatory response, good biocompatibility Effect

Inactive Publication Date: 2020-06-02
中国医科大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, limitations of this treatment approach include: second surgery to obtain donor nerves, limited sources of donor nerves, nerve dysfunction at the donor site, difficulty obtaining nerves of appropriate size from the patient, susceptibility to infection at the donor site, and neuromas Formation
However, the limitations of extracellular matrix conduits include the following three points: (1) they lack sufficient mechanical support and are easily compressed by surrounding tissues; The matrix structure is damaged; (3) It is difficult for the porcine nerve with fixed shape and size to match the nerve injury site

Method used

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  • Novel dual-layer nerve conduit and preparation method thereof
  • Novel dual-layer nerve conduit and preparation method thereof
  • Novel dual-layer nerve conduit and preparation method thereof

Examples

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preparation example Construction

[0047] A preparation method of a novel double-layered nerve conduit, the specific steps are as follows:

[0048](1) Take fresh porcine nerves, peel off the attached fat, etc., and cut them into 3cm segments with scissors.

[0049] (2) Rinse the nerve 3 times with deionized water shaking (15min each time).

[0050] (3) Repeat freeze-thaw three times, freeze at -80°C for 30 minutes, and then water bath at 37°C for 30 minutes.

[0051] (4) The porcine nerve was decellularized (the nerve was soaked in Triton X-100 with a mass concentration of 4% for 12 h; washed with deionized water for 3 times for 15 min each time; the nerve was soaked in sodium deoxycholate with a mass fraction of 3% for 24 h; Wash with ionized water 3 times, 15min each time; repeat the above steps twice).

[0052] (5) Put the obtained decellularized nerve in a mold, and place it in a -80°C refrigerator to embed the fixed nerve with deionized water, and chop the fixed nerve with a cryostat with a thickness of ...

Embodiment 1

[0060] 1. The specific steps of the bilayer acellular matrix conduit are as follows.

[0061] (1) Take fresh porcine nerves, peel off the attached fat, etc., and cut them into 3cm segments with scissors.

[0062] (2) Rinse the nerve 3 times with deionized water shaking (15min each time).

[0063] (3) Repeat freeze-thaw three times, freeze at -80°C for 30 minutes, and then water bath at 37°C for 30 minutes.

[0064] (4) The porcine nerve was decellularized (the nerve was soaked in Triton X-100 with a mass concentration of 4% for 12 h; washed with deionized water for 3 times for 15 min each time; the nerve was soaked in sodium deoxycholate with a mass fraction of 3% for 24 h; Ionized water wash 3 times, each 15min; repeat twice).

[0065] (5) Put the obtained decellularized nerve in a mold, and place it in a -80°C refrigerator to embed the fixed nerve with deionized water, and chop the fixed nerve with a cryostat with a thickness of one micrometer.

[0066] (6) Remove excess ...

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Abstract

The invention provides a novel dual-layer nerve conduit. The novel dual-layer nerve conduit consists of an electrospin decellularization pig nerve substrate conduit layer, and a PLCL layer arranged onthe outer layer of the electrospin decellularization pig nerve substrate conduit layer. Besides, the invention provides a preparation method of the novel dual-layer nerve conduit. The preparation method comprises the following steps of (1) pretreating pig nerves; (2) performing decellularization on the pretreated pig nerves; (3) performing crushing and drying treatment on nerves after being subjected to decellularization; (4) performing electrostatic spinning on the dual-layer nerve conduit layer; and (5) performing post-treatment on the dual-layer nerve conduit layer. The novel dual-layer nerve conduit prepared by the method disclosed by the invention has good biocompatibility and favorable machinery support; an inner-layer decellularization matrix contains various kinds of functional proteins, including collagen, proteoglycan, glycosaminoglycans and nerve growth factors, proliferation, migration and axon growth of schwann cells can be promoted, and the outer-layer PLCL can also effectively prevent hyperplasia of fibrous connective tissue and invasion of surrounding tissue.

Description

technical field [0001] The invention belongs to the technical field of biological materials and nerve repair, and relates to the preparation of tissue matrix materials, in particular to a novel double-layer nerve conduit and a preparation method thereof. Background technique [0002] Peripheral nerve injury can lead to partial or total loss of motor function, neuropathic pain, and sensory impairment, and despite rapid advances in microsurgical techniques, complete recovery of peripheral nerve function remains a great challenge. [0003] According to the recent research on nerve repair methods at home and abroad, when there is no nerve defect gap or the gap between the two ends of the nerve is short, the anastomosis of the broken ends is the most ideal treatment method. However, the limitation of this treatment method is that the distance between the broken ends is the largest. is 5mm. Autologous nerve grafts are considered the gold standard for repairing peripheral nerve in...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/18A61L27/36A61L27/50
CPCA61L27/18A61L27/3633A61L27/3687A61L27/3691A61L27/50A61L2430/32C08L67/04
Inventor 敖强毛小燕梁芳
Owner 中国医科大学
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