Preparation method of bletilla striata polysaccharide composite cytoskeleton

A technology of bletilla striata polysaccharides and cell scaffolds, applied in medical science, prostheses, etc., can solve the problems of uncontrollable pore structure of cell scaffolds and affect applications, and achieve good market prospects, good biocompatibility, and excellent network mechanical properties Effect

Active Publication Date: 2019-05-14
SOUTHWEAT UNIV OF SCI & TECH
View PDF18 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The uncontrollable defects of the current cell scaffold pore structure seriously affect its application in tissue engineering

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of bletilla striata polysaccharide composite cytoskeleton
  • Preparation method of bletilla striata polysaccharide composite cytoskeleton
  • Preparation method of bletilla striata polysaccharide composite cytoskeleton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing a bletilla striata polysaccharide composite cell scaffold, comprising the following steps:

[0028] Step 1. Put the tubers of Bletilla striata into the freezer, control the temperature between -15°C, and freeze for 4 hours; crush the tubers of Bletilla striata, the temperature is between -15°C and the pressure is controlled at 0.1MPa to obtain Bletilla striata powder; use a cyclone The collector collects the Bletilla striata powder under negative pressure; the Bletilla striata powder is dried by spray drying, and then passed through a 80-mesh sieve to obtain the Bletilla striata powder; in parts by weight, 20 parts of Bletilla striata powder is added to 800 parts of distilled water, at 100°C Stir at 500r / min for 60min for the first extraction, centrifuge at 3000r / min for 10min, collect the supernatant, add the solid residue to 600 parts of distilled water, and stir at 500r / min for 60min at 100°C for the second extraction. Centrifuge at 3000r / min f...

Embodiment 2

[0033] A method for preparing a bletilla striata polysaccharide composite cell scaffold, characterized in that it comprises the following steps:

[0034] Step 1. Put the tubers of Bletilla striata into the freezer, control the temperature between -20°C, and freeze for 6 hours; crush the tubers of Bletilla striata, the temperature is between -20°C and the pressure is controlled at 0.3MPa to obtain Bletilla striata powder; use a cyclone The collector collects the Bletilla striata powder under negative pressure; the Bletilla striata powder is dried by spray drying, and then passed through an 80-mesh sieve to obtain the Bletilla striata powder; in parts by weight, take 15 parts of Bletilla striata powder and add 800 parts of distilled water, at 100°C Stir at 500r / min for 60min for the first extraction, centrifuge at 3000r / min for 10min, collect the supernatant, add the solid residue to 650 parts of distilled water, and stir at 500r / min for 60min at 100°C for the second extraction. ...

Embodiment 3

[0039] A method for preparing a bletilla striata polysaccharide composite cell scaffold, comprising the following steps:

[0040] Step 1. Put the tubers of Bletilla striata into the freezer, control the temperature between -15°C, and freeze for 4 hours; crush the tubers of Bletilla striata, the temperature is between -15°C and the pressure is controlled at 0.1MPa to obtain Bletilla striata powder; use a cyclone The collector collects the Bletilla striata powder under negative pressure; the Bletilla striata powder is dried by spray drying, and then passed through a 80-mesh sieve to obtain the Bletilla striata powder; in parts by weight, 20 parts of Bletilla striata powder is added to 800 parts of distilled water, at 100°C Stir at 500r / min for 60min for the first extraction, centrifuge at 3000r / min for 10min, collect the supernatant, add the solid residue to 600 parts of distilled water, and stir at 500r / min for 60min at 100°C for the second extraction. Centrifuge at 3000r / min f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
quality scoreaaaaaaaaaa
voidageaaaaaaaaaa
Login to view more

Abstract

The invention discloses a preparation method of a bletilla striata polysaccharide composite cytoskeleton. The method comprises the steps that (1) bletilla striata polysaccharide is prepared; (2) oxidized konjac glucomannan is prepared; (3) the bletilla striata polysaccharide and the oxidized konjac glucomannan are taken to be added into distilled water, heating, stirring and uniform mixing are carried out, sufficient dissolution is carried out, then bubbles are removed by standing overnight at 4 DEG C, pre-freezing, freezing and vacuum drying are carried out to obtain the bletilla striata polysaccharide composite cytoskeleton. The preparation method of the bletilla striata polysaccharide composite cytoskeleton has the advantages that the cytoskeleton with a controllable void ratio and excellent network mechanical property is obtained by blending the bletilla striata polysaccharide and the oxidized konjac glucomannan, and the problems of the single raw material source, inhomogeneous porosity and uncontrollability of an existing cytoskeleton can be effectively solved; the provided bletilla striata polysaccharide composite cytoskeleton has good biocompatibility, tissue inductivity andnetwork mechanical property, has a good market prospect in the field of tissue engineering, and is conductive to the migration and proliferation of stem cells, vascular endothelial cells and the likeand the angiogenesis.

Description

technical field [0001] The invention relates to a preparation method of a cell scaffold, in particular to a preparation method of a bletilla striata polysaccharide composite cell scaffold. Background technique [0002] Bletilla striata belongs to the genus Bletillaceae, also known as lotus and grass, white root, snow as powder, etc. It has been used as traditional Chinese medicine for thousands of years. Safe and comfortable, degradable, non-polluting, and good biocompatibility. It is a good natural bioengineering material and can be used as a pharmaceutical excipient in pharmaceutical preparations. Bletilla striata bulbs contain a large amount of water-soluble polysaccharides of Bletilla striata, which is the main functional component of Bletilla striata. Modern research analysis shows that a large number of plant polysaccharides contained in Bletilla striata are about 40% to 50% in dry bulb tissue. These polysaccharides are mainly composed of β-1,4-mannose, β-1,4-glucose...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/26
Inventor 龙晓燕严全罗学刚
Owner SOUTHWEAT UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap