Method for laminating on surface of cement-based material by using sodium alginate-supported bacterial strain

A technology of cement-based materials and sodium alginate, which is applied in the field of solidification, can solve the problems of insufficient strength and pollution, and achieve the effects of low cost, excellent performance, and improved impermeability

Inactive Publication Date: 2010-06-30
SOUTHEAST UNIV
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Problems solved by technology

[0004] Technical problem: In order to solve the problem of insufficient strength and pollution in the restoration of cement-based materials in the prior art, the present invention provides a method for coating the surface of cement-based materials by using sodium alginate immobilized bacterial strains. The strain Bacillus pasteurii with carbonate mineralization properties makes it mineralize and deposit a calcium carbonate protective layer in the microenvironment provided by the natural polymer in sodium alginate as the carrier, using its own enzymatic properties

Method used

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  • Method for laminating on surface of cement-based material by using sodium alginate-supported bacterial strain
  • Method for laminating on surface of cement-based material by using sodium alginate-supported bacterial strain
  • Method for laminating on surface of cement-based material by using sodium alginate-supported bacterial strain

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Embodiment 1

[0028] Inoculate the strain Bacillus pasteurii into the Erlenmeyer flask containing the culture medium: the composition of the culture medium is shown in Table 1, shake culture in a constant temperature shaking incubator at 25-37°C, control the initial pH condition at 6.0-8.0, and cultivate for 24-36 The strains that had grown to the stationary phase were taken out within hours. Centrifuge the obtained bacterial liquid with a certain concentration at 5000rpm for 6min, and wash out the centrifuged bacterial cells with 200-500μl trace of newly prepared culture medium to obtain a concentration of 1.0×10 9 ~1.0×10 10 cell / mL highly concentrated bacterial solution, use a paintbrush (0.8-1cm wide) to gently smear the bacterial solution on the surface of a 3cm×3cm×3cm cement stone substrate, wait for the surface to dry, and then use another paintbrush to paint the sodium alginate The mass volume concentration of the viscous solution is 10g / L and spread on the surface of the substrat...

Embodiment 2

[0034] The strain Bacillus pasteurii was inoculated into a Erlenmeyer flask containing medium (the medium composition is shown in Table 2), and cultured in a constant temperature shaking incubator at 25-37°C, with the initial pH controlled at 6.0-8.0, and cultured for 24-37°C. 36 hours to remove. The next steps are the same as those in Example 1. The resulting treated cement stone will be removed from the composite film to measure its capillary water absorption coefficient K is 0.0004 ~ 0.0008g.cm -2 .s -1 / 2 .

[0035] Medium composition used in the embodiment 2 of table 2

[0036]

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Abstract

The invention provides a method for laminating on the surface of a cement-based material by using a sodium alginate-supported bacterial strain. In the method, biomineralization of microorganisms in nature is fully used; a bacterial strain, namely Bacillus pasteurii, having a carbonate mineralization characteristic is selected and used to slowly generate compact and hard calcium carbonate on the surface of the base material in a microenvironment provided by sodium alginate serving as a carrier by using the enzymatic characteristic per se, so that the permeability resistance of the base material is greatly improved, and the base material is protected in situ and strengthened; in addition, the sodium alginate can be gradually decomposed by bacteria with the prolonging of the time so as not to prevent the generated calcium carbonate from tightly combining with the base material; and the sodium alginate serving as the carrier can directly fix the bacterial strain onto the surface of the base material, so that the base material can be protected in situ and strengthened. The method is suitable for practical application, and is a protection method with environmental friendliness, simple process and low cost.

Description

technical field [0001] Inspired by the principle of biomineralization, the patent of the present invention selects a carbonate mineralization strain and provides suitable conditions to make it use its own enzymatic characteristics in the microenvironment provided by the natural polymer sodium alginate as a carrier. A layer of calcium carbonate is mineralized and deposited on the surface of cement-based materials, which plays the role of protection and reinforcement, and belongs to the field of microbial technology application research. Background technique [0002] As the most widely used building material-cement-based composite material, such as concrete, due to the existence of various aggressive media in its service environment, the surface properties gradually decrease, causing problems such as surface looseness and peeling, micro cracks and so on. If the surface defects are not repaired in time, the aggressive medium will further penetrate into the interior of the mater...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B41/50C04B41/65C12N1/20C12R1/07
Inventor 钱春香王瑞兴王剑云
Owner SOUTHEAST UNIV
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