Method for preparing Fe2O3 nanometer particles by using collagen as biological mineralization template

Abstract

The invention discloses a method for preparing Fe2O3 nanometer particles by using collagen as a biological mineralization template. The method comprises the following steps of (1) preparing recombinant collagen through a biological genetic engineering technique: (a) determining the sequence of the recombinant collagen; (b) synthesizing nucleic acid for coding the recombinant collagen; and (c) preparing and purifying the recombinant collagen; and (2) preparing an alpha-Fe2O3 nanometer material: (a) compounding a uniform mixed solution of the recombinant collagen and ferric trichloride hexahydrate; (b) preparing nanoscale alpha-Fe2O3 through a hydrothermal reaction kettle; and (c) performing purification on the prepared nanometer material, and then performing drying preservation on the purified nanometer material. According to the method disclosed by the invention, the recombinant collagen is used as the biological template, the ferric trichloride hexahydrate is used as a raw material, and by a hydrothermal method, the alpha-Fe2O3 nanometer material of which the magnitude and the topology are controllable is prepared. In the whole synthetic process of the method disclosed by the invention, any other chemical reagents do not need to be added, or post-treatment is not needed, the method is simple and convenient and easy to operate and has great application prospects, and a basis can be provided for the large-scale production of the alpha-Fe2O3 nanometer material.

Description

technical field [0001] The present invention relates to Fe 2 o 3 A method for preparing nanoparticles, in particular to a method for preparing Fe with collagen as a biomineralization template 2 o 3 The nano particle method belongs to the technical field of biological inorganic material preparation. Background technique [0002] Biomineralization refers to the process in which organisms generate inorganic minerals through the regulation of biological macromolecules. It is a bridge between inorganic and biological. The biggest difference from general mineralization is that it is a process in which organisms transform ions in solution into solid-phase minerals at specific locations, under certain physical and chemical conditions, and under the control or influence of biological organic substances. The formation of bones, scales, teeth, etc. are relatively common biomineralization processes in nature. Unlike industrial production conditions, biomineralization does not requi...

AI Technical Summary

Problems solved by technology

Some of these methods require the addition of toxic and harmful chemical reagents during the synthesis process, and some require post-processing such as heating up to remove solvents or templates to obtain products, which are relatively complicated, and have the disadvantages of time-consuming, energy-consuming, and high cost.

At the same time, due to the current understanding of α-Fe 2 o 3 The basic research on the shape and size controllability of nanoparticles is still not systematic and sufficient. Therefore, in order to meet different needs, a simple, easy-to-control, and green method is used to regulate α-Fe 2 o 3 The shape and size of nanoparticles are of great importance

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