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Hydroxyapatite/phase transformation lysozyme coating hybrid material and preparation method thereof

A technology of hydroxyapatite and hybrid materials, which is applied in coatings, pharmaceutical formulations, medical science, etc., can solve the problems of single source of raw materials, non-single preparation components, and fast biodegradation, and achieve simple and controllable operation process, Controllable crystal morphology and high osteoinductive effect

Active Publication Date: 2017-09-01
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these preparation technologies still have disadvantages such as high price, fast biodegradation, low mechanical strength, not single preparation components, complicated process, and are suitable for a small number of substrates and single source of raw materials. Therefore, a new, simple, green and environmentally friendly, and Bio-coatings applicable to a variety of substrates for biomimetic mineralization to prepare hydroxyapatite remain a challenge for scientists

Method used

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  • Hydroxyapatite/phase transformation lysozyme coating hybrid material and preparation method thereof
  • Hydroxyapatite/phase transformation lysozyme coating hybrid material and preparation method thereof
  • Hydroxyapatite/phase transformation lysozyme coating hybrid material and preparation method thereof

Examples

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Effect test

Embodiment 1

[0037] Add 0.1433g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(hydroxymethylaminomethane) buffer solution with a pH value of 7.4, adjust the pH value to 5.8 with NaOH, and prepare 50mmol / L of tris(2- Carboxyethyl) phosphine tris buffer solution; 20mg lysozyme was added to 10mL 10mmol / L tris buffer solution with a pH value of 7.4 to prepare 2mg / mL trihydroxymethyl aminomethane buffer solution of lysozyme Methylaminomethane buffer solution; mix 0.2mL 50mmol / L tris(2-carboxyethyl)phosphine tris buffer solution with 0.2mL 2mg / mL lysozyme tris buffer solution Uniformly, a titanium sheet with a diameter of 1 cm is in contact with the surface of the mixed solution, and left at room temperature for 50 minutes, a layer of lysozyme two-dimensional nano-film (PTL) is adhered on the surface of the titanium sheet, and its thickness is about 50nm. Fully immerse the titanium sheet with lysozyme two-dimensional nano film on the surface in 0.02mol / L calcium chloride aqueous sol...

Embodiment 2

[0040] In the present embodiment, the titanium sheet of embodiment 1 is used ZrO 2 Replacement, other steps are identical with embodiment 1, obtain HAp@PTL coated hybrid material (see Figure 8 ).

Embodiment 3

[0042] In this example, the titanium sheet in Example 1 was replaced with a medical catheter, and the other steps were the same as in Example 1 to obtain a HAp@PTL coated hybrid material (see Figure 9 ).

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Abstract

The invention discloses a hydroxyapatite / phase transformation lysozyme coating hybrid material and a preparation method thereof. The preparation method comprises the following steps: simulating biological mineralization in natural world, taking lysozyme as a protein template, chelating calcium ions onto the surface of a base material by utilizing functional groups abundant on the surface, then simulating a biological mineralization process in vitro by virtue of electrostatic interaction, successfully inducing nucleation of hydroxyapatite, and crystallizing, so that the hydroxyapatite / phase transformation lysozyme coating hybrid material which is similar to a natural bone in ingredient and structure is prepared. The hydroxyapatite / phase transformation lysozyme coating hybrid material is strong in mechanical stability, large in superficial area, good in biocompatibility and high in bone conductibility, can be taken as a tissue repairing material for guiding formation of a new tissue and induced mineralization of a bone, is expected to become a novel hard tissue substitute material and is simple in preparation process, mild in conditions, controllable in crystal morphology, environment-friendly and low in cost, so that the hydroxyapatite / phase transformation lysozyme coating hybrid material is applicable to preparation of hydroxyapatite on the surfaces of multiple base materials and has a broad application prospect.

Description

technical field [0001] The invention belongs to the technical field of preparation of bone tissue repair materials, and in particular relates to a hydroxyapatite coating hybrid material prepared by phase transition lysozyme and a preparation method thereof. Background technique [0002] Hydroxyapatite (HAp), the molecular formula is Ca 10 (PO 4 ) 6 (OH) 2 , is a compound composed of organic and inorganic nano-hybrids, which has a similar composition to the inorganic matrix of human and animal hard tissue bones and teeth, and has good biocompatibility, bioactivity, osteoconduction and bone The combined ability has great potential application prospects in contemporary biomedicine and tissue engineering. However, most of the currently prepared hydroxyapatite materials are inert and lack interfacial activity. Therefore, the key to the successful preparation of biologically active hydroxyapatite organic-inorganic hybrid materials is to design a flexible and Controlled interf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/32A61L27/34A61L27/50
CPCA61L27/32A61L27/34A61L27/50A61L2400/18A61L2420/02A61L2420/04A61L2430/02C08L89/00
Inventor 杨鹏吴倩哈媛
Owner SHAANXI NORMAL UNIV
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