40 results about "Sagittaria trifolia" patented technology

The invention discloses a herbicide-resistant gene StGSTF8 in sagittaria trifolia and application of the herbicide-resistant gene StGSTF8, and belongs to the technical field of genetic engineering. The bensulfuron methyl resistance gene StGSTF8 (glutathione-S-transferase F8) is identified from sagittaria trifolia for the first time by means of proteomics, molecular biology, transgenic function verification and the like, the nucleotide sequence is as shown in SEQ ID NO: 1, the coded amino acid sequence is as shown in SEQ ID NO: 2, and the nucleotide sequence is as shown in SEQ ID NO: 1. It is proved that the strain endows plants with resistance through mediating herbicide detoxification metabolism and oxidative stress resistance, and reference is provided for improvement of the resistance of plants to sulfonylurea herbicides and research and development of novel herbicides.

The invention discloses an agrobacterium rhizogenes-mediated arrowhead genetic transformation method, and belongs to the technical field of plant genetic engineering. An agrobacterium rhizogenes-mediated arrowhead genetic transformation system is established through stem tip tissue culture without inducing calluses, and the method comprises the following steps: sterilizing arrowhead stem tips and preparing sterile stem tips; culturing and activating agrobacterium rhizogenes; infecting the stem tips with agrobacterium rhizogenes; performing stem tip tissue culture to induce rooting; performing positive identification on new roots; acclimatizing and transplanting to obtain positive seedballs, and accelerating germination and regenerating to obtain positive plants. The arrowhead genetic transformation method based on agrobacterium rhizogenes, established by the invention, has important significance on gene function research and molecular breeding of arrowhead.