A tissue culture rapid propagation method of endangered orchid cymbidium floribundum

By using the same culture medium with a single hormone TDZ in the tissue culture of Cymbidium goeringii, the problems of complex operation, high cost and low efficiency in Cymbidium goeringii propagation have been solved, and efficient and simple tissue culture rapid propagation has been achieved, meeting the needs of large-scale seedling production.

CN122319944APending Publication Date: 2026-07-03GUIZHOU BOTANICAL GARDEN (GUIZHOU INST OF HORTICULTURAL SCI GUIZHOU INST OF BOTANY)
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUIZHOU BOTANICAL GARDEN (GUIZHOU INST OF HORTICULTURAL SCI GUIZHOU INST OF BOTANY)
Filing Date
2026-04-14
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing propagation methods for Cymbidium goeringii are cumbersome, costly, have low propagation efficiency, low transplant survival rate, and are limited by external conditions, making it difficult to achieve large-scale industrialized seedling production.

Method used

The proliferation and differentiation induction of Oryza sativa protocorms were induced using a single hormone, thiafenthril (TDZ), on the same culture medium, simplifying the operation process. Through optimization of MS medium formulation and light conditions, efficient proliferation and differentiation were achieved, and the tissue culture seedlings survived directly after transplanting without hardening.

Benefits of technology

The operation process has been simplified, the propagation efficiency has been improved, the original bulb has a high proliferation coefficient, the culture cycle is short, the survival rate of transplanted tissue culture seedlings reaches 98%, it is not limited by seasons and external climate, the production cost has been reduced, and large-scale industrialized seedling production of Cymbidium goeringii has been realized.

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Abstract

This invention discloses a rapid propagation method for the endangered orchid *Cymbidium goeringii* via tissue culture, belonging to the field of plant tissue culture technology. The method includes capsule sterilization and aseptic seed germination, protocorm proliferation, protocorm differentiation, rooting culture, and transplantation of tissue culture seedlings. Its key feature is that protocorm proliferation induction uses MS medium containing 0.05 mg / L–1.0 mg / L TDZ, and protocorm adventitious bud induction is continuously performed on the same medium without the need for transfer or medium change. This invention is the first to apply TDZ to the rapid propagation system of *Cymbidium goeringii*, achieving both proliferation and differentiation steps on the same medium, greatly simplifying the operation process and reducing costs. Protocorm proliferation is 5–6 times after 21 days of culture, and can reach more than 15 times after 60 days, with high rooting and transplant survival rates; the survival rate reaches 98% after 30 days of transplanting. This method is simple to operate, has a short cycle, a high propagation coefficient, and low cost, making it suitable for large-scale industrial seedling production and of great significance for the protection and sustainable utilization of *Cymbidium goeringii* germplasm resources.
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Citation Information

Patent Citations

  • Tissue culture rapid propagation method of cymbidium floribundum

    CN107711506A

  • Rapid propagation method of endangered wild plant cymbidium floribundum

    CN113796315A