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Method for novel variety culture and rapid tissue culture propagation of paphiopedilum

A technology for fast propagation and new varieties of tissue culture, applied in horticultural methods, botanical equipment and methods, plant genetic improvement and other directions, can solve the problems of low reproduction coefficient, difficulty in reproduction, long reproduction cycle, etc. The effect of shortening the germination time and improving the germination rate

Inactive Publication Date: 2018-06-15
董春燕 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to the technical defects existing in the prior art, the technical problem to be solved by the present invention is to provide a method for cultivating a new variety of Paphiopedilum and a method for tissue culture and rapid propagation, so as to obtain a high-efficiency Paphiopedilum with high germination rate, fast seedling growth, and good seedling quality. Orchid hybrid breeding and seedling propagation technology to solve the existing problems of low germination rate, difficulty in seedling formation, long propagation cycle, low reproduction coefficient and difficult reproduction in the cultivation and propagation of new Paphiopedilum species

Method used

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Embodiment 1

[0026] A method for cultivating a new variety of Paphiopedilum and rapid propagation through tissue culture, wherein the cultivating and rapid propagation of a new Paphiopedilum variety comprises the following steps:

[0027] (1) Artificial cross-pollination: Select two Paphiopedilum parents for hybridization, and the two Paphiopedilum parents are the male parent and the female parent for hybridization, and the pollen of the male parent is collected and pollinated on the stigma of the female parent to complete the pollination;

[0028] (2) Sterile sowing and seed germination: select plump hybrid fruits as explants, soak the explants in 70%-80% alcohol for 30-60s, and then place them in 0.1%-0.2% alcohol Sterilize in mercuric chloride solution for 10-20 minutes, rinse the sterilized explants with sterile water for 4-5 times, cut them open to obtain powdered seeds, inoculate the powdered seeds on the seed germination medium and cultivate them until they grow into seedlings; The ...

Embodiment 2

[0039] A method for cultivating a new variety of Paphiopedilum and rapid propagation through tissue culture, wherein the cultivating and rapid propagation of a new Paphiopedilum variety comprises the following steps:

[0040] (1) Artificial cross-pollination: Select two Paphiopedilum parents for hybridization, and the two Paphiopedilum parents are the male parent and the female parent for hybridization, and the pollen of the male parent is collected and pollinated on the stigma of the female parent to complete the pollination;

[0041] (2) Aseptic sowing and seed germination: select plump hybridized fruits as explants, soak the explants in 70% alcohol for 30s, and then sterilize them in 0.1% mercuric chloride solution for 10 minutes. The sterilized explants were rinsed 4 times with sterile water and then cut to obtain powdered seeds, which were inoculated on the seed germination medium and cultivated until they grew into seedlings; the culture conditions for seed germination we...

Embodiment 3

[0052] A method for cultivating a new variety of Paphiopedilum and rapid propagation through tissue culture, wherein the cultivating and rapid propagation of a new Paphiopedilum variety comprises the following steps:

[0053] (1) Artificial cross-pollination: Select two Paphiopedilum parents for hybridization, and the two Paphiopedilum parents are the male parent and the female parent for hybridization, and the pollen of the male parent is collected and pollinated on the stigma of the female parent to complete the pollination;

[0054] (2) Aseptic sowing and seed germination: select plump hybrid fruits as explants, soak the explants in 75% alcohol for 40 seconds, and then sterilize them in 0.13% mercuric chloride solution for 13 minutes. The sterilized explants were rinsed 4 times with sterile water and then cut to obtain powdered seeds, which were inoculated on the seed germination medium and cultivated until they grew into seedlings; the culture conditions for seed germinatio...

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Abstract

The invention discloses a method for novel variety culture and rapid tissue culture propagation of paphiopedilum and relates to the technical field of breeding and tissue culture of paphiopedilum. Themethod for novel variety culture and rapid tissue culture propagation of paphiopedilum comprises the following steps: performing artificial hybridization pollination, performing sterile seed sowing and seed germination, strengthening seedlings, promoting rooting, and transplanting test tube seedlings. The method is good in operability, high in application value, high in hybridization success rate, and very effective for excellent cross parents of paphiopedilum in flowering asynchronism; in the rapid tissue culture propagation process, a unique seed germination culture medium and a seedling strengthening rooting culture medium are used, so that the germination rate of paphiopedilum hybridization seeds is increased, seedlings can grow rapidly, and the seedlings are good in quality. The method for novel variety culture and rapid tissue culture propagation of paphiopedilum, which is disclosed by the invention, has the characteristics of being good in operability, high in application value, high in hybridization success rate, and the like, the problems that conventional novel variety culture and rapid tissue culture propagation process of paphiopedilum is low in germination rate, hardin seedling growth, long in propagation cycle, low in propagation coefficient, large in propagation difficulty, and the like, are solved, an effective way is provided for production of paphiopedilum seedlings, and industrial planting can be achieved.

Description

technical field [0001] The invention relates to the technical field of Paphiopedilum breeding and tissue culture, in particular to a method for cultivating a new variety of Paphiopedilum and tissue culture rapid propagation. Background technique [0002] Paphiopedilum, also known as "slipper orchid" and "Cinderella orchid", is a general term for plants of the genus Paphiopedilum in the family Orchidaceae. The ornamental value is very popular in the market. The cultivation and breeding of Paphiopedilum has a long history. Up to now, it has developed a huge number of hybrid varieties and has the most fans all over the world. In recent years, my country's Paphiopedilum industry has developed rapidly, and the market demand has increased rapidly. However, due to the destruction of the ecological environment and over-harvesting, Paphiopedilum has become one of the most endangered plant species in the world, and all wild species are listed in Appendix I of the Convention on Inter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01H1/04
CPCA01H1/02A01H1/04A01H4/001A01H4/008
Inventor 董春燕李天宇
Owner 董春燕
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