Dendrobium unicum aseptic seeding and test tube seedling tecnnology

A technology of aseptic seeding and unicorn, which is applied in botany equipment and methods, horticulture, gardening tools/equipment, etc., can solve the problems of incomplete embryo development, large market demand, slow reproduction speed, etc., and achieve good growth and widespread Representative, simple and effective formula

Inactive Publication Date: 2004-11-03
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, the products of Dendrobium orchids must be produced through artificial seeding and tissue culture propagation, and the market demand is large. The propagation of Dendrobium orchids is mostly carried out by branching, and the reproduction speed is slow. However, due to the incomplete embryo development of Dendrobium orchid seeds, it is extremely difficult to produce them under natural conditions. Germination, but possible on suitable artificial media

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] 1. Materials: Select the vigorously grown Dendrobium unicornus mother plant during flowering for artificial pollination, and when the fruit matures in 120 days after pollination, it is used as an explant for sowing.

[0013] 2. Aseptic sowing: remove the fruit, soak it in 75% alcohol for 30 seconds, put it in 0.1% mercuric acid solution for 20 minutes, rinse it with sterile water for 4-5 times, cut the fruit, and inoculate the white fruit with an inoculation needle. The powdery embryos are inoculated on the seed germination medium for about 30 days, the embryos germinate, and the complete plantlets are formed in about 60 days, and the plantlets are transferred to the strong seedling medium in time.

[0014] 3. Tissue culture: Culture the protocorms or seedlings produced by aseptic sowing on the proliferation medium to form a mixed tissue of buds and protocorms, which can be subcultured on the same medium, and the mixed tissue can be inoculated into strong seedlings On t...

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Abstract

The present invention relates to the abacterial seeding and tissue culture of Dendrobium unicum. The Dendrobium as important orchid family has about 1000 varieties, and Dendrobium unicum is beautiful and unique and has very high enjoying value. Up to now, Dendrobium unicum is propagated via offshoot process in slow rate and is hard to germinate naturally owing to its incomplete development of embryo. The present invention obtains great amount of test tube seedling via utilizing artificial nutritious liquid and abacterial seeding and small seedling proliferation and obtains complete plants via rooting culture. The said seedling growing technology has the advantages of high germination rate, fast seedling growing rate, high seedling quality, etc.

Description

technical field [0001] The invention relates to the aseptic sowing and tissue culture technology of Dendrobium orchid. Background technique [0002] There are more than 1,000 species of Dendrobium, and there are more than 70 species in my country. Most of them have high ornamental value and there are many hybrid varieties. They are one of the most important species in the world's orchid industry. Dendrobium unicorn is a native species native to Vietnam. It has bright colors and unique flower shapes, and has high ornamental value. A few wild plants are extremely popular in the market. At present, the products of Dendrobium orchids must be produced through artificial seeding and tissue culture propagation, and the market demand is large. The propagation of Dendrobium orchids is mostly carried out by branching, and the reproduction speed is slow. However, due to the incomplete embryo development of Dendrobium orchid seeds, it is extremely difficult to produce them under natural...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 段俊曾宋君陈之林陈建通
Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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