Test-tube breeding method for Nothodoritis germchit

A technology for test-tube propagation and Elephant Trunk Orchid, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of incomplete development of Elephant Trunk Orchid seed embryos, difficulty in germination, and reduction of wild resource mining, etc., to achieve a germination rate High, low cost, fast seedling effect

Inactive Publication Date: 2009-11-25
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the destruction of the ecological environment, the wild resources of Elephant Trunk Orchid are less and less, and because the embryo of Elephant Trunk Orchid is not fully developed, it is extremely difficult to germinate under natural conditions. At present, there is no method for breeding Elephant Trunk Orchid seedlings at home and abroad. It is reported that, therefore, the research and development of a test tube propagation method capable of cultivating a large number of Elephant Trunk Orchid seedlings has great significance and broad application prospects for reducing the further excavation of wild resources, meeting the needs of the market, and carrying out ecological reconstruction back to nature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Material: Select a robust Elephant Trunk Orchid mother plant for artificial pollination when flowering, and use the fruit as an explant for sowing after 90 days of pollination.

[0020] (2) a, aseptic sowing: sterilize 10 minutes in 0.1% mercuric chloride solution after soaking in 70% alcohol for 30 seconds, cut the fruit after rinsing with sterile water 4 times, inoculate the powdered embryo into the improved Knudson On the seed germination medium of coconut milk with 5% additional volume percentage of C medium, the embryo germinated in about 30 days, and formed small plants in about 80 days; the seed germination rate was 60%, and the seedling rate was 50%; the improved Knudson C The medium contains ammonium sulfate per liter [(NH 4 ) 2 SO 4 ]500mg, potassium dihydrogen phosphate (KH 2 PO 4 ) 250mg, magnesium sulfate (MgSO 4 .7H 2 O) 250mg, calcium nitrate [Ca 3 (PO4) 2 ] 1000mg, manganese sulfate (MnSO 4 .4H 2 O) 7.5mg, ferrous sulfate (FeSO 4 .7H 2 O)...

Embodiment 2

[0026] (1) Materials: Select a robust Elephant Trunk Orchid mother plant for artificial pollination when flowering, and use it as an explant for sowing 100 days after pollination.

[0027] (2) a, aseptic sowing: sterilize 15 minutes in 0.15% mercuric chloride solution after soaking in 75% alcohol for 45 seconds, cut the fruit after rinsing with sterile water 4 times, inoculate the powdery embryo into the improved Knudson On the seed germination medium of coconut milk with 7.5% additional volume percentage of C medium, the success rate of disinfection is 95%, the embryo germinates in about 25 days, and forms small plants in about 70 days; the seed germination rate is 70%, and the seedling rate is 60%; The modified Knudson C medium described above contains per liter of ammonium sulfate [(NH 4 ) 2 SO 4 ]400mg; Potassium dihydrogen phosphate (KH 2 PO 4 ) 200mg; Magnesium sulfate (MgSO 4 .7H 2 O) 200mg; Calcium nitrate [Ca 3 (PO4) 2 ]800mg; Manganese sulfate (MnSO 4 .4H 2...

Embodiment 3

[0033] (1) Materials: Select a robust Elephant Trunk Orchid mother plant for artificial pollination when flowering, and the fruit 120 days after pollination is used as explants for sowing.

[0034] (2) a, aseptic sowing: sterilize 20 minutes in 0.2% mercuric chloride solution after soaking in 80% alcohol for 60 seconds, cut the fruit after 5 times of rinsing with sterile water, and inoculate the powdery embryo into the improved Knudson On the seed germination medium with 10% coconut milk added to the C medium, the success rate of disinfection is 100%, the embryos germinate in about 25 days, and small plants are formed in about 60 days; the seed germination rate is 80%, and the seedling rate is 70%; Ammonium sulfate [(NH 4 ) 2 SO 4 ]600mg; Potassium dihydrogen phosphate (KH 2 PO 4 ) 300mg; Magnesium sulfate (MgSO 4 .7H 2 O) 300mg; Calcium nitrate [Ca 3 (PO4) 2 ] 1400mg; Manganese sulfate (MnSO 4 .4H 2 O) 10mg; ferrous sulfate (FeSO 4 .7H 2 O) 35mg; sucrose 30g, agar...

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Abstract

The present invention provides a test-tube breeding method for Nothodoritis germchit, selecting a Nothodoritis stock plant growing vigorously to implement an artificial pollination when flowering, using a fruitage without dehiscence growing until basic ripeness after the pollination as an explant, seeding sterilely or proliferating and differentiation culturing an original bulbodium, good seedling culturing and transplanting and the like. Implementing a sterile seeding and a following generation proliferation to the original bulbodium by employing a confecting-specially culture medium to obtain a young seedling, and culturing the young seedling in a confecting-specially culture medium and transplating. The method of the invention has a high seed sprouting rate, a rapid seedling formation, a good seedling quality, a low cost and the like, it is capable of obtaining a great amount of test-tube plantlets in a short time, and a transplantation survival of the seedling is more than 90%. The invention provides an effective approach for producing the Nothodoritis germchit.

Description

technical field [0001] The invention relates to a plant seedling propagation method, in particular to a seedling test-tube propagation method of Elephant Trunk Orchid. Background technique [0002] Elephant Trunk Orchid (Notodoritis zhejiangensis Z.H.Tsi) belongs to Orchidaceae Elephant Trunk Orchid. It is the only species in this genus. It is mainly distributed in Tianmu Mountain, Lin'an, Zhejiang, my country and Ningbo. It is born in mountain forests or forests at an altitude of 350-900 meters. On the edge branches, it is similar to Doritis and Phalaenopsis in shape, but its sepals and petals are white, with purple horizontal stripes inside, very gorgeous and unique. It is also one of the few precious orchid germplasm resources in China that have not been lost to foreign countries. [0003] Due to the destruction of the ecological environment, the wild resources of Elephant Trunk Orchid are becoming less and less, and because the embryo of Elephant Trunk Orchid is not full...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 段俊陈之林曾宋君吴坤林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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