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Sterile seeding and tissue cultivating technology for Vanda

A technology of aseptic sowing and tissue culture, applied in the fields of botany equipment and methods, horticulture, gardening tools/equipment, etc., can solve the problems of aseptic sowing and tissue culture, large-scale production and patent application of Wandai orchid , to achieve the effect of less investment, fast emergence and high stability

Inactive Publication Date: 2005-03-02
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no reports of aseptic sowing and tissue culture, large-scale production and patent application of wandailan at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Material: select the mother plant of Vanda coerulea (Vanda coerulea) with strong growth and carry out self-pollination when flowering, and when the fruit is 140 days after pollination, the fruit is basically mature, and the seeds in the mature fruit are used as explants for sowing.

[0025] 2. Aseptic sowing: Take the basically mature fruit, soak it in 75% alcohol for 30 seconds, put it in 0.1% mercuric chloride solution for 20 minutes, rinse it with sterile water for 4 times, cut the fruit, and inoculate the white fruit with an inoculation needle. Powdered seeds were inoculated into seed germination medium: V&W medium + 6-benzylpurine 1.0 mg / L + naphthaleneacetic acid 0.5 mg / L 10% + 20% coconut water. Embryos germinate in about 40 days, and a complete plant is formed in about 60 days. When the sterile seedlings in the medium are too dense and the seedlings are small, strong seedlings can be cultured on the same medium.

[0026] 3. Proliferation of clustered buds: ...

Embodiment 2

[0031] 1. Materials: Vanda coerulea and Ascocentrum ampullaceum are used as parents. When flowering, a strong mother plant is selected for hybridization. The fruit is basically mature 130 days after pollination as an explant for sowing.

[0032] 2. Aseptic sowing: Take the basically mature fruit, soak it in 75% alcohol for 30 seconds, put it in 0.1% mercuric acid solution for 20 minutes, rinse it with sterile water for 5 times, cut the fruit, and inoculate the white fruit with an inoculation needle. Powdered seeds were inoculated into seed germination medium: V&W medium + 6-benzylpurine 1.0 mg / L + NAA 0.2 mg / L 10% coconut water. Embryos germinate in about 30-days, and complete plantlets form in about 60 days. When the sterile seedlings in the medium are too dense and the seedlings are small, strong seedlings can be cultured on the same medium.

[0033] 3. Proliferation of clustered buds: In order to obtain more seedlings, use the shoot tips or stem nodes of sterile seedlings...

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PUM

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Abstract

The present invention relates to the sterilized seeding and tissue cultivation technology of Vanda as one of four main kinds of ornamental orchid. The present invention obtains great amount of test seedling of Vanda through sterilized seeding in artificial culture medium, and forms complete plant via proliferation and rooting culture of stem tip or stem node of abacterial seedling. The present invention has practical operation and high application value.

Description

technical field [0001] The invention relates to the aseptic sowing and tissue culture techniques of wandailan. Background technique [0002] Vanda, also known as Vanda orchid, is known as one of the "four major ornamental orchids" together with Cattleya, Dendrobium, and Phalaenopsis. Vandella is native to tropical and subtropical regions of Asia. There are more than 70 native species and more than 600 hybrid species of this genus in the world. In my country, 14 species and 1 variant have been found, mainly distributed in the southern region. [0003] Vandella is a uniaxial stem-like cayenne with distinct stems and aerial roots. Its roots are thick and strong, not only can rely on its own developed root system to make the whole plant epiphyte on other plants or rocks and support its stem to grow upward, but also the developed root system is also responsible for the absorption of water and nutrients, especially the roots of some species It turns green when exposed to the out...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 段俊曾宋君陈之林黄向力温铁龙
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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