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An artificial and seed technology, applied in germination equipment and other directions, can solve the problems of slow growth rate, poor seedling quality, and low seedling emergence rate.
Inactive Publication Date: 2007-11-28
云南省农业科学院高山经济植物研究所
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Bilberry seeds have special requirements for light during germination, and it is difficult to germinate under dark conditions. The conventional method of raising bilberry seedlings is to spread seeds on the surface of the soil. This method of raising seedlings is difficult due to unbalanced light, water and nutrition. Artificial regulation, resulting in slow growth, the general seedling cycle is at least one year, and the emergence rate is low, and the quality of seedlings is poor
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[0005] Below in conjunction with embodiment further illustrate the specific content of the present invention.
[0006] The vaccinia seeds after sterilization are inoculated into MS medium under aseptic conditions, and cultured under tissue culture conditions. In order to improve the sterilization effect of the seeds and avoid the seeds from being damaged during the sterilization process, when the bilberry seeds are sterilized, the ripe bilberry fruits can be collected first, preferably 6-9 mature bilberry fruits, and washed with a washing machine first. After soaking in the powder liquid for 10-30 minutes, rinse with running water for 0.5-1.5 hours, disinfect with 70% ethanol for 10-30 seconds on the ultra-clean workbench, rinse 2-6 times with sterile water, and use 0.1% HgCl 2 Disinfect for 5 to 10 minutes, rinse with sterile water for 2 to 6 times, and after absorbing the water, under sterile conditions, crush the bilberry fruit, take out the seeds and inoculate them into MS...
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Abstract
The present invention provides artificial blueberry seedling raising process, and features that the blueberry seed after being sterilized is inoculated to MS culture medium under aseptic condition for culturing under tissue culture condition. The present invention meets the specific requirement of blueberry seed during germination, and has simple operation, short seedling raising period, high germination rate and other advantages. The present invention makes it possible to obtain great amount of blueberry seedlings in short term by means of industrial production.
Description
technical field [0001] The invention relates to a method for artificial seedling cultivation of bilberry. Background technique [0002] Blueberry (blueberry), also known as blueberry, blueberry, blueberry, is a plant of the genus Vaccinium (Ericaceae) of the Rhododendron family (Ericaceae). Bilberry fruit is blue, with an average weight of 0.5-2.5g and a maximum of 5g. The color of the fruit is beautiful and pleasing to the eye, the flesh is delicate, the seeds are very small, and the edible rate is 100%. It is light and fragrant, sweet and sour, and is a good fresh food. In addition to the conventional sugar, acid and V C outside, rich in V E , V A , V B , SOD, arbutin, protein, anthocyanins, dietary fiber and rich in potassium, iron, zinc, calcium and other mineral elements. According to the analysis, the anthocyanin content in the fruit is as high as 163mg / 100g fresh fruit, V E The content is 9.3μg / 100g fresh fruit, and the total amino acid content is 2.54%. Bilber...
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