Method for aseptically sowing and rapidly propagating paphiopedilum helenae

A sterile seeding and rapid technology, applied in the field of plant biology, can solve the problems of easy browning propagation speed, slow reproduction speed, low germination rate, etc., to achieve resource protection and sustainable utilization, high seed germination rate, and seed good seedling quality

Active Publication Date: 2017-04-19
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Routine propagation of P. helenensis can be done by division, but the propagation speed is slow and the reproduction rate is low; and in the wild, P. Implants are difficult to decontaminate, easy to brown, and slow to proliferate, making tissue culture extremely difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Artificial pollination and seed acquisition

[0022] In ex situ conservation greenhouse, the mother plant of Paphiopedilum helenae Aver. which was successfully introduced and domesticated was selected for out-plant artificial pollination. The pollination time was selected 1 day after the flower finished unfolding. When pollinating, remove the lips of the flower for easy operation, and use a sterilized toothpick to pick the pollen grains and stick them on the stigma of another flower. The date of pollination is then recorded. The success rate of artificial pollination is 95%. The powdered seeds 120 days after pollination were then selected for aseptic sowing of the explants.

[0023] (2) Sterilization and aseptic seeding of explants

[0024] The capsules 120 days after pollination were soaked in 70% alcohol by volume for 30 seconds, then disinfected with 0.1% mercuric solution for 20 minutes, rinsed 4 times with sterile water, and then cut open the fruit, and ino...

Embodiment 2

[0034] (1) Artificial pollination and seed acquisition

[0035] In ex situ conservation greenhouse, the mother plant of Paphiopedilum helenae Aver. which was successfully introduced and domesticated was selected for out-plant artificial pollination. The pollination time was selected 3 days after the flowers finished unfolding. When pollinating, remove the lips of the flower for easy operation, and use a sterilized toothpick to pick the pollen grains and stick them on the stigma of another flower. The date of pollination is then recorded. The success rate of artificial pollination is 100%. The powdered seeds 150 days after pollination were then selected for aseptic sowing of the explants.

[0036] (2) Sterilization and aseptic seeding of explants

[0037] The capsules 150 days after pollination were soaked in 75% alcohol by volume for 45 seconds, then disinfected with 0.15% mercuric solution for 15 minutes, rinsed with sterile water for 5 times, and then cut open the fruit,...

Embodiment 3

[0047] (1) Artificial pollination and seed acquisition

[0048] In ex situ conservation greenhouse, the mother plant of Paphiopedilum helenae Aver. which was successfully introduced and domesticated was selected for out-plant artificial pollination. The pollination time was selected 6 days after the flowers finished unfolding. When pollinating, remove the lips of the flower for easy operation, and use a sterilized toothpick to pick the pollen grains and stick them on the stigma of another flower. The date of pollination is then recorded. The success rate of artificial pollination is 85%. The powdered seeds 180 days after pollination were then selected for aseptic sowing of the explants.

[0049] (2) Sterilization and aseptic seeding of explants

[0050] Capsules 180 days after pollination were soaked in 80% alcohol by volume for 30 seconds, then disinfected with 0.2% mercuric solution for 10 minutes, rinsed with sterile water for 5 times, then cut open the fruit, and inocu...

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PUM

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Abstract

The invention discloses a method for aseptically sowing and rapidly propagating paphiopedilum helenae. The method is characterized by taking seeds in appropriate embryonic age as explants, aseptically sowing the seeds, differentiating protocorms, proliferating protocorm-like bodies and culturing strong seedlings on a unique culture medium, and producing a large quantity of high-quality paphiopedilum helenae seedlings in a short time. The method has the advantages of high seed germination rate, rapid seedling emergence and high quality of seedlings; the paphiopedilum helenae seedlings can be applied to the supply market, ex-situ conservation and natural regression; the resource protection and the sustainable utilization of paphiopedilum helenae can be facilitated.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of plant biology, and in particular relates to a rapid propagation method for aseptic sowing of P. [0003] Background technique: [0004] Paphiopedilum helenae Aver., distributed in western Guangxi and northern Vietnam, has high ornamental value. Due to the destruction of its habitat and artificial over-excavation, the number of wild plants has been extremely scarce, and has been listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and banned from trading. ) is listed as a critically endangered species (CR) in the Red List and needs to be protected urgently. In order to protect the resources of Helen Philistine, it is the key to multiply enough seedlings. The conventional propagation of P. Helensis can be carried out by ramets, but the reproduction speed is slow and the reproduction rate is low; in the wild, P. Implants are difficult t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君邓莹吴坤林郑枫张建霞张新华马国华段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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