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Multi-flower paphiopedilum test-tube seedling cultivation method

A cultivation method and technology of test-tube seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as the difficulty of aseptic sowing and tissue culture, and the difficulty of seed test-tube cultivation

Active Publication Date: 2014-04-09
ZHEJIANG SENHE SEED
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, Paphiopedilum is difficult to sow aseptically and tissue culture, and it is one of the most difficult genus for seed tube culture in Orchidaceae.

Method used

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  • Multi-flower paphiopedilum test-tube seedling cultivation method

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Experimental program
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Embodiment 1

[0023] 1) Fruit pod acquisition: The mature pods of three multi-flowered Paphiopedilum species, King, Mike, and Lady, were selected as experimental materials. When flowering, robust mother plants were selected for artificial cross-pollination, and mature pods were harvested 120 days after pollination.

[0024] 2) Aseptic sowing: Soak pod explants in 75% alcohol for 30 seconds, then sterilize with 0.1% mercuric chloride for 15-20 minutes. Rinse 5 times with sterile water, cut the fruit pods on the ultra-clean workbench, and spread the powdered mature seeds evenly on the sowing medium; the sowing medium is MS medium + fish peptone 3g / L + activated carbon 0.5 g / L+apple crushed material 100ml / L, PH5.4-5.6; cultivate in dark for about 30 days, change to light culture when the seeds start to germinate, the light intensity is 200Lx, and the light time is 10h / d. After one week, the light intensity increases to 500Lx. Time 10h / d; At this time, the germinated seeds gradually turn green,...

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Abstract

The invention provides a multi-flower paphiopedilum test-tube seedling cultivation method, belonging to the technical field of plant growing. The method is characterized by comprising the following steps: 1) pod acquisition; 2) aseptic seeding; 3) multiplication subculture; 4) root and strong seedling culture; and 5) test-tube seedling transplantation. The multi-flower paphiopedilum test-tube seedling cultivation method has the characteristics of high germination rate, short seedling emergence cycle, robust seedlings, developed root systems, good seedling quality, low production cost and the like, can be used for large-scale industrialized production of paphiopedilum and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant planting, in particular to a method for cultivating test-tube seedlings of multi-flowered Paphiopedilum. Background technique [0002] Paphiopedilum, also known as slipper orchid, is a perennial herb of Orchidaceae. There are more than 70 species of this genus in the world, and it is a national first-class protected plant. "Washington Convention" (Convention on International Trade in Endangered Species of Wild Fauna and Flora, Convention on International Trade in Endangered Species of Wild Fauna and Flora, CITES) includes all Paphiopedilum species in its appendix, which is strictly enforced by the international community Protect. The cultivation and breeding of Paphiopedilum has a long history, and there are a large number of hybrid varieties at present. The multi-flower series Paphiopedilum, such as Mike, King, and Lady, has several flowers on each stem. It is one of the most popular species of C...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 郑勇平王春陈任文展张慕欢
Owner ZHEJIANG SENHE SEED
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