Tissue-culture quick-propagation method of sarcandra drug germchit

A technology of tissue culture rapid propagation and nine-section tea, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems such as the inability to use nine-section tea for rapid propagation of seedlings, and achieve strong resistance, fast emergence, and practical strong effect

Active Publication Date: 2006-04-26
GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, if the tissue culture only reaches the callus stage but cannot germinate and take root, it cannot be used for the rapid breeding of Jiujie tea.
There has not been any report on the in vitro rapid propagation of Jiujie tea through the method of clustering buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Material collection: Take the segmented stem segment of Sarcandra glabra (Thunb.) Nakai as explant.

[0019] 2. Disinfection of the explants: Select the vigorously growing plant branches, rinse them under tap water, cut off the leaves, and wipe the surface with 75% alcohol cotton balls. Cut about 2cm of sectioned stems, soak them in 75% alcohol for 30 seconds on the ultra-clean workbench, rinse them with sterile water, and then soak them in 0.1% mercuric dichloride solution (add 0.5ml Tween 80 per liter) After 20 minutes, rinse with sterile water.

[0020] 3. Insert the sterilized explants into the prepared primary medium: MS plus 6-BA 2.0 mg / L, sucrose 30 g / L and agar 7.0 g / L. The light is 12 hours a day, the light intensity is 2000Lux, the temperature is 25°C, and the culture is carried out for 35 days to induce clustered buds, and each explant can induce 4 to 6 buds.

[0021] 4. Proliferation culture: Divide the induced cluster buds into individual buds or small...

Embodiment 2

[0025] 1. Material collection: Take the terminal buds of Jiujie tea as explants.

[0026] 2. Disinfection of explants: Cut out the terminal buds of about 2cm long and healthy plants, rinse them under tap water, cut off the leaves, and wipe the surface with 70% alcohol cotton balls. On the ultra-clean workbench, soak with 75% alcohol for 30 seconds, rinse with sterile water, then disinfect with 0.1% mercuric dichloride solution (add 1ml Tween 80 per liter) for 20 minutes, rinse with sterile water, and the success rate of disinfection There is no significant difference with Example 1.

[0027] 3. Insert the sterilized explants into the prepared primary medium: MS plus 6-BA 4.0 mg / L plus sucrose 30 g / L plus agar 7.0 g / L. The light is 12 hours a day, the light intensity is 2000Lux, the temperature is 25°C, and the culture is carried out for 35 days to induce clustered buds, and each explant can induce 2 to 3 buds.

[0028] 4. Proliferation culture: Divide the induced cluster bud...

Embodiment 3

[0032] Operation with embodiment 1, the difference is that the 0.1% mercuric dichloride solution (adding 0.7ml Tween 80 per liter) in the explant surface disinfection was processed for 15 minutes, and the explant disinfection success rate was slightly lower than that in embodiment 1 20 minutes to process. The medium and culture conditions used for the induction, proliferation and rooting culture of clustered buds are the same as in Example 1, and the induction and proliferation rate, root number and plant growth status of clustered buds are all indistinguishable from those in Example 1.

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Abstract

A method for tissue culturing and fast breeding the breed sprout of sarcandra Folium et Ramulus crude drugs, relating to the seedling method of tissue culturing and fast breeding the sarcandra Folium et Ramulus crude drugs through the biological tissue culture and asexual propagation technique, comprising the following five steps: choosing the sarcandra Folium et Ramulus with basistipes or apical as explant for surface sterilization; first generation culturing for inducing the clump budding; breeding culture; root growing culturing; refining sprouts and replanting. The invention is characterized by the fast emergence of seedlings, high stability, strong sprouts, high quality of seed sprout, strong resistant, good growing condition and strong practicability, etc; which solves the sarcandra Folium et Ramulus crude drugs seed supply for the planting, and provides safeguard for the GAP stander management for the sarcandra Folium et Ramulus crude drugs planting, and provides quality guarantee for the medical production which applying the sarcandra Folium et Ramulus crude drugs as raw material.

Description

technical field [0001] The invention relates to a plant regeneration method through tissue culture technology, in particular to a method for tissue culture rapid propagation and seedling cultivation of Jiujie tea medicinal material through biological tissue culture asexual reproduction technology to obtain stable plant property. Background technique [0002] Nine-section tea (Sarcandra glabra (Thunb.) Nakai), aliases: grass coral, elderflower, bamboo grass, swollen joint wind. It is a semi-shrub of Chrysanthemum orchidaceae, a perennial evergreen herb or subshrub, with a plant height of 50-120 cm. The flowering period is from June to July every year, and the fruiting period is from August to September every year. Born in ravines, valleys and wetlands, distributed in South China, East China, South Central and Southwest China. Contains chemical ingredients with medicinal value. The "Qingre Xiaoyanning Capsule" that has been successfully developed is prepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张明永朱淑颖陆颂规李美茹彭红英潘小平
Owner GUANGZHOU BAIYUNSHAN JINGXIUTANG PHARM CO LTD
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