Sterile sowing and tissue culture method of tree orchid

A technology of aseptic sowing and tissue culture, applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve the problems of incomplete seed development and difficult germination of orchids, and achieve strong resistance, good seedling quality, The effect of high survival rate

Inactive Publication Date: 2004-10-13
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that tree orchid seeds are difficult to germinate in the natural state due to incomplete embryo development, and to develop a method for large-scale propagation of tree orchid seedlings

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: aseptic sowing and tissue culture propagation of tree orchid

[0026] 1. Select a robust mother plant for artificial pollination when it blooms, and take the fruit after 120 days of pollination as an explant;

[0027] 2. After soaking in 75% alcohol for 30 seconds, put it in 10% sodium hypochlorite solution for disinfection for 20 minutes, rinse it with sterile water for 4 times, cut the fruit, and use an inoculation needle to spread the white powdery embryos evenly into the culture medium. 100 milliliters of coconut water contained in each liter of the medium, 7 grams of agar, 20 grams of sucrose, and the rest of the ingredients are N 6 , 4 bottles were sown with one fruit, the embryo germinated in 30 days, and a complete plant was formed in 60 days;

[0028] Description: The coconut water is obtained by filtering the coconut water from fresh coconuts through multi-layer gauze. The pH of the culture medium is 5.5, the culture temperature is (26±2)° C., ...

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Abstract

The present invention relates to a sterile sowing and tissue culture method of epidendrum radians. The invention adopts artificial nutrient liquor and utilizes sterile sowing method to oblain lots of test-tube seedlings, and utilizes the stem apex or stipes of sterile seedling-plant to make multiplication of clustered bud, then utilizes rootage and culture to form whole plant.

Description

technical field [0001] The invention relates to a method for aseptic sowing and tissue culture of tree orchid. technical background [0002] Epidendrum, also known as Epidendrum, is one of the largest genera in Orchidaceae. There are more than 1,000 species of this genus in the world, epiphytic or terrestrial, mostly produced in tropical America. According to the different habits of the species It can be cultivated in high temperature, medium temperature or low temperature greenhouse. Some species have a dormancy period, while others do not. Its stem also varies greatly, some have pseudobulbs, some have reed-like erect stems, and there are a few leaves at the top of the pseudobulbs. The flower stem of the tree orchid plant is born on the top of the stem. There are several to many flowers. The flowers are large or small. The sepals and petals are very thin and narrow, but the lips are hypertrophic, the base is deeply concave, and the stamens are very developed. belongs to....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 曾宋君段俊黄向力
Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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