Early embryo sterile sowing and tissue culture method of phaius hainanensis

A technology of sterile seeding and tissue culture, applied in botany equipment and methods, horticultural methods, horticulture, etc., can solve the problems of difficult germination of seeds, shedding of hybrids, incompatibility of hybrids, etc., and achieve good growth and quality of seedlings good, resistant effect

Inactive Publication Date: 2005-09-14
SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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  • Summary
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AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the seeds of Hainan Hedinglan are difficult to germinate in the natural state and hybridization is incompatible, and the fruit of the hybrid species falls off before maturity, and develop a method for large-scale propagation of Hainan Hedinglan seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Early Embryo Aseptic Sowing and Tissue Culture Propagation of Hainan Hedinglan

[0028] 1. Take off the fruit that has grown for 65 days and make explants;

[0029] 2. After soaking in 75% alcohol for 30 seconds, put it in 10% sodium hypochlorite solution for disinfection for 20 minutes, rinse it with sterile water for 4 times, cut the fruit, and inoculate the green immature embryo into the medium with an inoculation needle. The above medium contains 2 mg of 6-benzyl purine per liter, 0.3 mg of naphthalene acetic acid, 150 ml of coconut water, 7 g of agar, 30 g of sucrose, and the rest of the ingredients are MS. Embryos germinate in 130 days and complete plants form in 160 days ;

[0030] Description: The coconut water is obtained by filtering the coconut water from fresh coconuts through multi-layer gauze. The pH of the medium is 5.5, the culture temperature is (26±2)° C., the light intensity is 1500-2000 lx, and the light is 12 hours / day.

[0031] 3. Utilize the as...

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Abstract

The present invention relates to an early embryo sterile sowing and tissue culture method of phaius hainanensis. It adopts artificial nutrient liquor and utilizes sterile sowing method of early embryo to obtain lots of test-tube seedlings, and then utilizes the sterile seedling-plant to make multiplication of original bulbodium, and utilizes the rootage culture so as to form whole plant.

Description

technical field [0001] The invention relates to a method for aseptic sowing and tissue culture of early embryos of Hainan Hedinglan. technical background [0002] Hainan Crane Orchid (Phaius hainanensis) is a perennial herb native to Hainan Province, my country. The plant is tall and large, with broad-lanceolate leaves and longitudinal fan-shaped folds. The flower stems are erect, in racemes, with 10-20 flowers, the perianth and petals are ivory white on both sides, the flowers are large, the flowering period is late spring and early summer, and the flowering period can last up to January. Due to the lush growth of Hainan Hedinglan, the scapes are erect, the flower shape is beautiful, and the color is bright, it has high ornamental value. In warm places, it can be planted under garden trees, on the side of trellises, beside stones and streams for ornamental gardens; in cool places, it can be potted for indoor decoration. In addition, the pseudobulb of Hainan Hedinglan can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 段俊曾宋君黄向力
Owner SOUTH CHINA PLANT INST CHINESE ACAD OF SCI
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