Tissue culturing and rapid propagation method of dendrobiumtosaense makino seedlings

A tissue culture and rapid technology, applied in the field of plant biology, can solve the problems of no patents and literature reports on the reproduction of Dendrobium huangshii seedlings, and achieve the effects of fast reproduction speed, good quality of seedlings, and uniform traits of offspring.

Pending Publication Date: 2017-11-03
NANJING XIANCAOTANG BIOLOGICAL TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no patents and literature reports on the propagation of Dendrobium officinalis in the world

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: a kind of rapid propagation method of Dendrobium officinale seedling tissue culture

[0023] (1) Material selection and handling

[0024] The young shoots of Dendrobium officinale with strong plant growth and strong growth potential were selected as explants. Before cutting the explants 28 days, water once with 500 times of carbendazim and spray the leaves, and then water once and spray the leaves with 4000 times of 72% agricultural streptomycin soluble powder one week later. Then repeat the above steps for two weeks. Before inoculation, use a 75% alcohol-sterilized scalpel to cut out lateral buds with a length of 2 cm as explants.

[0025] (2) Disinfection of explants

[0026] Remove the leaves of the excised explants on the ultra-clean workbench, soak them in 70% alcohol for 10 seconds, then disinfect them with 0.1% mercuric chloride solution for 5 minutes, rinse them with sterile water for 4 times, and then rinse them with 0.1% mercuric chloride solu...

Embodiment 2

[0034] Embodiment 2: A kind of rapid propagation method of dendrobium seedling tissue culture

[0035] (1) Material selection and handling

[0036] The young shoots of Dendrobium officinale with strong plant growth and strong growth potential were selected as explants. Before cutting the explant for 28 days, water once with 600 times of carbendazim and spray the leaves, and then water once and spray the leaves with 3500 times of 72% agricultural streptomycin soluble powder one week later. Then repeat the above steps for two weeks. Before inoculation, use a 75% alcohol-sterilized scalpel to cut out lateral buds with a length of 3 cm as explants.

[0037] (2) Disinfection of explants

[0038] Remove the leaves of the excised explants on the ultra-clean workbench, soak them in 75% alcohol for 20 seconds, then disinfect them with 0.1% mercuric chloride solution for 7.5 minutes, wash them with sterile water for 5 times, and then rinse them with 0.1% mercuric chloride solution. ...

Embodiment 3

[0046] Embodiment 3: A kind of rapid propagation method of dendrobium seedling tissue culture

[0047] (1) Material selection and handling

[0048] The young shoots of Dendrobium officinale with strong plant growth and strong growth potential were selected as explants. Before cutting the explant 28 days, water once with 800 times of carbendazim and spray the leaves, and then water once with 4000 times of 72% agricultural streptomycin soluble powder and spray the leaves after one week. Then repeat the above steps for two weeks. Before inoculation, use a scalpel sterilized by 75% alcohol to cut out the lateral buds with a length of 4 cm as explants.

[0049] (2) Disinfection of explants

[0050] Remove the leaves of the excised explants on the ultra-clean workbench, first soak them in 80% alcohol for 30 seconds, then sterilize them with 0.1% mercury liter solution for 10 minutes, rinse them with sterile water for 6 times, and then use 0.1% mercuric chloride The solution was ...

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PUM

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Abstract

The invention discloses a tissue culturing and rapid propagation method of dendrobiumtosaense makino seedlings. The method comprises the steps of: selection and treatment of raw materials, sterilization of explants, induction and proliferation of adventitious shoots, rooting and hardening-seedling culture and the like. In the step of induction and proliferation of the adventitious shoots, cluster shoots are cut into single shoot, and then the single shoot is inoculated on a rooting and hardening-seedling culture medium for culturing to obtain a complete plant; the step of the induction and proliferation of the adventitious shoots is as follows: based on improved MS ( Murashige and Skoog), adding 3.0 to 5.0 milligrams/liters of 6-benzyladenine, 3.0 to 5.0 milligrams/liters of kinetin, 1.0 to 2.0 milligrams/liters of naphthaleneacetic acid and 10 to 20 percent of coconut juice; the rooting and hardening-seedling culture medium is the improved MS culture medium, based on the improved MS, 0.5 to 1.0 milligrams/liters of naphthaleneacetic acid, 0.5 to 1.0 milligrams/liters of indolebutyric acid, 50 to 150 grams/liters of banana juice and 0.5 to 1.0 grams/liters of activated carbon are added, and the improved MS culture medium is 1/2 of MS. According to the method disclosed by the invention, tissue culturing and propagation on raw green shoots can be carried out in a whole year, and the method has the characteristics of quick propagation speed, excellent quality of seedlings, uniform offspring characteristics and the like; moreover, the tissue culturing and propagation can be completed by only needing simple tissue culturing equipment.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a rapid propagation method of dendrobium dendrobium seedling tissue culture. Background technique [0002] Dendrobium ( Dendrobium tosaense Makino) belongs to the genus Dendrobium orchidaceae. It is only produced in Jiangxi, Guangdong, Taiwan, Japan and other places in my country. It is often mistaken for Dendrobium candidum. It has been widely used as a traditional Chinese medicine in Japan and Taiwan, and its output is higher than that of Dendrobium candidum. , has the effects of anti-tumor, anti-aging, enhancing human immunity and dilating blood vessels; at the same time, the yellow-green flowers of Dendrobium officinale have high ornamental value and can be used in potted plants for ornamental purposes, with broad application prospects. However, due to the scarcity of Dendrobium dendrobium provenance, the development of its industry has been seriously restricted. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 曾宋君史月龙吴坤林郑枫
Owner NANJING XIANCAOTANG BIOLOGICAL TECH CO LTD
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