Tissue culture reproduction method of Chinese evergreen

A technology of tissue culture and induction medium, which is applied in the field of tissue culture and propagation of Sclerostifolia, can solve the problems such as no tissue culture of Scleracea, and achieve the effect of accelerating the propagation speed, maintaining excellent traits, and high stability

Inactive Publication Date: 2011-09-14
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on tissue culture, large-scale production of seedlings and patent application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] a. Material selection: Select young stems of Aglaonema rotundum as explants during the growing season.

[0025] b. Disinfection of explants and induction of adventitious buds: Rinse the young stems of R. rotundum under tap water, soak them for 30 seconds in 70% alcohol by volume fraction, and then use mass volume percentage concentration Disinfect with 0.1% mercuric chloride solution for 8 minutes, rinse with sterile water 4 times, cut into segments about 1 cm long, inoculate them on the induction medium, take out the segments after 5 days and use 0.1% mercuric chloride in mass percent concentration again The solution was sterilized for 8 minutes, rinsed with sterile water for 5 times, and then transferred to a new induction medium. The success rate of disinfection was 71%, and adventitious buds could be formed on the explants in 60 days. Contains 2.0mg of 6-benzylpurine, 0.2mg of naphthaleneacetic acid, 20g of sucrose, 6g of agar, and the rest are MS;

[0026] c. Subc...

Embodiment 2

[0033] a. Material selection: Select young stems of Aglaonema Silver King as explants during the growing season.

[0034] b. Disinfection of explants and induction of adventitious buds: Rinse the young stems of Grasshopper spp. under tap water, soak them in 75% alcohol for 45 seconds, and then sterilize them with 0.15% mercuric chloride solution for 10 minutes , washed 5 times with sterile water, cut into 1 cm-long segments, inoculated on the induction medium, took out the segments after 6 days, sterilized with 0.15% mercuric chloride solution for 8 minutes, rinsed with sterile water 5 times, and then In the new induction medium, the disinfection success rate can reach 78%, and adventitious buds can be formed on the explants in 60 days. The induction medium contains 3.0 mg of 6-benzylpurine and 0.5 mg of naphthaleneacetic acid per liter. milligrams, 25 grams of sucrose, 7 grams of agar, and the rest are MS;

[0035] c. Subculture proliferation: the induced adventitious buds a...

Embodiment 3

[0042] a. Material selection: select young stems of Aglaonema Tembaga Super Red as explants during the growing season.

[0043] b. Disinfection of explants and induction of adventitious buds: Rinse the young stems of Sclerophyllum superrhiza under tap water, soak them in 80% alcohol for 60 seconds, and then sterilize them with 0.2% mercuric chloride solution for 8 minutes , washed 5 times with sterile water, cut into segments about 1 cm long, inoculated on the induction medium, took out the segments after 7 days, sterilized again with 0.1% mercuric chloride solution for 8 minutes, rinsed with sterile water 5 times, and then In the new induction medium, the disinfection success rate can reach 79%, and adventitious buds can be formed on the explants in 50 days. The induction medium contains 5.0 mg of 6-benzylpurine and 1.0 mg of naphthaleneacetic acid per liter. milligrams, 30 grams of sucrose, 7 grams of agar, and the rest are MS;

[0044] c. Subculture proliferation: the indu...

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PUM

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Abstract

The invention discloses a tissue culture reproduction method of Chinese evergreen and aims to provide a tissue culture reproduction method of the Chinese evergreen with high reproduction speed, mass test seedling obtainment in a short term, little damage to a mother plant and the maintenance of mother plant fine traits. The method comprises the following steps: selecting young stems of eugenic Chinese evergreen as explants, disinfecting the explants, carrying out the adventitious-bud induction of an induction culture medium and coupling adventitious buds into a reproduction culture medium forsuccessive transfer reproduction; cutting and inoculating the buds to a rooting culture medium for rooting culture while the buds grow to the height of 3cm to 5cm; training the seedlings under natural light for 7 days to 10 days when the test seedlings grow to the height of 6cm to 7cm; and taking the test seedlings out, washing the rooting culture medium, planting in a volumetric mixture medium comprising sand, perlite, peat soil, and the like and obtaining planting seedlings after conventional culture. The invention can provide markets with mass Chinese evergreen test seedlings.

Description

Technical field: [0001] The invention relates to a method for tissue culture and propagation of Grass officinalis. Background technique: [0002] Aglaonema is a general name for plants of the genus Aglaonema in the family Araceae. It is a perennial evergreen herb native to India, Thailand, Vietnam, the Philippines, Malaysia and Indonesia in Southeast Asia. The plant height of rough rib grass is 20-150 cm. Leaves alternate, lanceolate to narrowly ovate, 10-45 cm long and 4-16 cm wide. The flowers are small and inconspicuous, the inflorescences are spathes, spathes are white or greenish white, and the fruits are berries, which turn red when ripe. Grass has high ornamental value and is an excellent foliage flower, which can be used as a high-grade foliage plant for indoor and garden viewing. [0003] There are three kinds of propagation methods for R. scutellaria, cutting and seed propagation. The speed of branch propagation is slow, and although seed propagation is an effec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 张建霞吴坤林李虬曾宋君陈之林段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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