Ficus tikoua rapid propagation method
A ground loquat and fast technology, applied in the field of tissue culture and rapid propagation of ground loquat, can solve the problems of no loquat tissue culture research and patent application, etc., and achieve the effects of effective protection, development and utilization, simple process, and fast seedling emergence.
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Embodiment 1
[0029] Embodiment 1, the loquat aseptic seedling of tissue culture rapid propagation ground
[0030] 1. Culture medium preparation and sterilization
[0031] Primary medium: MS+6-BA0.8mg / L+IAA0.3mg / L;
[0032] Subculture medium: N6 macroelement + MS trace element + MS organic component + MS iron salt + 6-BA1.0mg / L + KT0.5mg / L + IAA0.2mg / L + paclobutrazol (CCC) 1.0mg / L;
[0033] Rooting medium: MS+IAA0.2mg / L+NAA0.5mg / L+KT0.5mg / L+paclobutrazol (CCC) 0.5mg / L;
[0034] The above medium was sterilized at 121°C for 20 minutes.
[0035] 2. Primary culture
[0036] Take the terminal buds or axillary buds with stems of loquat (Ficus tikoua) (the total length of stems and buds is 2-3cm), first wash them with washing powder, wash them with sterile water 5-7 times on a sterile operating table, and use 70% Soak in alcohol for 1 minute, then sterilize in 0.1% mercuric chloride solution for 12 to 18 minutes, rinse with sterile water for 5 to 6 times, transfer the terminal buds or axillar...
Embodiment 2
[0047] Embodiment 2, the aseptic seedling of loquat in tissue culture rapid propagation ground
[0048] Media Preparation and Sterilization
[0049] Primary medium: MS+6-BA1.0mg / L+IAA0.5mg / L;
[0050] Subculture medium: N6 massive + MS trace + MS organic + MS iron salt + 6-BA1.0mg / L + KT0.3mg / L + IAA0.2mg / L + paclobutrazol (CCC) 0.5mg / L;
[0051] Rooting medium: MS+IAA0.3mg / L+NAA0.3mg / L+KT0.5mg / L+paclobutrazol (CCC) 0.5mg / L.
[0052] The above medium was sterilized at 121°C for 20 minutes;
[0053] Take ground loquat (Ficus tikoua) terminal buds or stem segments, the basic operation method is the same as in Example 1, and the multiplication and rooting effect is slightly worse than that in Example 1, but it can also be achieved very well.
Embodiment 3
[0054] Embodiment 3, tissue culture rapid propagation ground loquat aseptic seedling
[0055] Primary medium: MS+6-BA0.5-1.5mg / L+IAA0.1-0.8mg / L
[0056] The subculture medium is: macroelements of N6 medium + trace elements of MS medium + organic components of MS medium + iron salt of MS medium + 6-BA1.0-2.5mg / L + KT0.2- 0.5mg / L+IAA0.1-0.8mg / L+paclobutrazol 0.5-2.0mg / L.
[0057] The rooting culture medium is: MS+IAA0.1-0.8mg / L+NAA0.1-0.8mg / L+KT0.5-1.0mg / L+paclobutrazol 0.2-1.0mg / L.
[0058] Within the concentration ranges of the above-mentioned several culture media, the operation steps in Example 1 can be followed, and satisfactory results can basically be achieved.
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