A kind of rapid propagation method of longan

A longan and fast technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as low reproduction rate, seedlings cannot meet production needs, etc., achieve high reproduction coefficient, eliminate top advantages, and high efficiency.

Inactive Publication Date: 2016-05-04
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Layering and grafting are often used for conventional longan propagation, and the reproduction rate is low. It often occurs that seedlings of good varieties cannot meet the production demand. Therefore, it is of great significance to explore the rapid propagation technology of longan

Method used

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  • A kind of rapid propagation method of longan
  • A kind of rapid propagation method of longan
  • A kind of rapid propagation method of longan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of rapid propagation method of longan, comprises the following steps:

[0034] Step 1, selection and disinfection of explants:

[0035] Take the longan lateral buds, soak them in 2% detergent aqueous solution for 5 minutes, and then rinse them with tap water for 10 minutes. Sterilize in the mercury solution for 8min, rinse with sterile water 3 times after the disinfection is completed, and finally absorb the moisture on the surface of the longan lateral buds with sterilized filter paper to obtain the explant;

[0036] Step two, adventitious bud cultivation:

[0037] The explants obtained in step 1 were inoculated into the adventitious bud medium, and the culture temperature was 23°C, the light intensity was 1400 lux, the light time was 10 hours / day, and the relative humidity was 40% and cultivated for 30 days to obtain Adventitious buds; the pH of the adventitious bud medium is 5.8, and its formula is: 1 / 2MS, 0.8mg / L zeatin, 0.1mg / L6-BA, 25g / L sucrose and 3.8g / ...

Embodiment 2

[0046] A kind of rapid propagation method of longan, comprises the following steps:

[0047] Step 1, selection and disinfection of explants:

[0048]Take the longan lateral buds, soak them in 2% detergent aqueous solution for 15 minutes, and then rinse them with tap water for 20 minutes. Sterilize in the mercury solution for 10min, after the disinfection is completed, use sterile water to rinse 5 times, and finally absorb the moisture on the surface of the longan lateral buds with sterilized filter paper to obtain the explant;

[0049] Step two, adventitious bud cultivation:

[0050] Inoculate the explants obtained in step 1 into the adventitious bud culture medium, and cultivate them under the condition that the culture temperature is 23-26°C, the light intensity is 1400-2000lux, the light time is 12 hours / day, and the relative humidity is 40%. In 30 days, adventitious buds were obtained; the pH of the adventitious bud medium was 5.8, and its formula was: 1 / 2MS, 2mg / L zeati...

Embodiment 3

[0059] A kind of rapid propagation method of longan, comprises the following steps:

[0060] Step 1, selection and disinfection of explants:

[0061] Take the longan lateral buds, soak them in 2% detergent solution for 10 minutes, then rinse them with tap water for 15 minutes, add 0.1% of 2g / LPVP and 4-6g / L Tween-20 after rinsing Disinfect in the mercuric chloride solution for 9min, after the disinfection is completed, use sterile water to rinse 4 times, and finally absorb the moisture on the surface of the longan lateral buds with sterilized filter paper to obtain the explant;

[0062] Step two, adventitious bud cultivation:

[0063] The explants obtained in step 1 were inoculated into the adventitious bud medium, and the culture temperature was 24°C, the light intensity was 1600 lux, the light time was 11 hours / day, and the relative humidity was 40% and cultivated for 30 days to obtain Adventitious buds; the pH of the adventitious bud medium is 5.8, and its formula is: 1 / 2...

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Abstract

The invention provides a method for rapid propagation of longan, which is characterized in that it comprises the following steps: taking longan lateral buds, soaking, washing, disinfecting, rerinsing, and absorbing the water on the surface of longan lateral buds to obtain explants; explanting The body was cut into small sections with axillary buds, inoculated into adventitious bud medium and cultivated until the lateral buds differentiated into adventitious buds; then the adventitious buds were placed in clustered bud medium and cultivated for 40 days to obtain robust plants; the robust plants were placed in rooting culture Cultured in medium for 40 days to obtain complete plants with roots; 2-4 days after hardening, the seedlings were taken out, the root medium was washed, and transplanted into sandy soil with good ventilation and low light, and transplanted after one and a half months of cultivation. Plant a field. The method is very suitable for industrialized production, has the advantages of good formula effect, fast germination, short cultivation time, vigorous plant growth, strong operability and high application value. It has the characteristics of less investment and high output.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, in particular to a method for rapid propagation of longan. Background technique [0002] Longan is a rare and precious specialty in the south subtropical zone of our country. Li Shizhen in the Ming Dynasty once commented that "longan is the best source of wealth". According to analysis, longan pulp contains 12.38-22.55% of total sugar, 3.85-10.16% of reducing sugar, 0.096-0.109% of total acid, and 43.12-163.7 mg of vitamin C per 100 g of pulp. In addition to being eaten fresh, longan can also be processed into dried, canned, decocted and so on. Longan has many functions such as strengthening yang and replenishing qi, nourishing the heart and spleen, nourishing blood and calming the nerves, moisturizing and beautifying the skin, etc. Modern medical practice has proved that it also has the effects of beautifying and prolonging life. The fruit is rich in nutrition and has been loved by p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李刚王晓峰徐函兵缪剑华韦坤华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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