Quick breeding method for butterfly orchid high quality sprout

A Phalaenopsis, high-quality technology, applied in the field of rapid propagation of high-quality Phalaenopsis seedlings, can solve the problems of rapid propagation and large-scale production of high-quality Phalaenopsis seedlings that have not existed before, and achieve simple and affordable technology, high genetic stability, and rapid emergence Effect

Inactive Publication Date: 2005-03-02
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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AI Technical Summary

Problems solved by technology

[0004] At present, many studies have been carried out on the aseptic sowing and tissue culture of Phalaenopsis at home and abroad, but there is no report on the rapid propagation and large-scale production of Phalaenopsis high-quality seedlings through the cluster bud approach.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Material: Phalaenopsis Taida Salu (Phalaenopsis Taida Salu "Sunset Red")

[0016] 2. Disinfection of explants: take the pedicel of the safflower bud, rinse it with tap water, cut off the segment with dormant buds 2-3 cm, soak it with 70% alcohol on the ultra-clean workbench for 1 minute, Then sterilize with 0.1% (weight percent) mercuric chloride solution for 15 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the incision, and cut into 1-2 cm long with dormant buds Cut sections were inoculated on pedicel seedling induction medium.

[0017] 3. Induction of pedicel seedlings: the induction medium of pedicel seedlings is VW+6-benzylpurine 10 mg / liter+naphthalene acetic acid 0.5 mg / liter+sodium citrate 150 mg / liter, 15-day dormant buds start to grow, and in culture When the temperature is (28±2)℃, pedicel seedlings with leaves are mostly formed.

[0018] 4. Proliferation of clustered buds: when cultur...

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PUM

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Abstract

The present invention relates to the quick breeding method of high quality seedling of Phalaenopsis, known as 'queen orchid' and the culture medium therewith. The present invention uses the flower stem section of Phalaenopsis as explant and induces flower stem seedling with unique culture medium and performs proliferation with fascicular bud stem tip or stem section to produce high quality seedling of Phalaenopsis. The method is simple, practical, feasible and high in application value.

Description

technical field [0001] The invention relates to a method for rapid propagation of high-quality seedlings of Phalaenopsis and a used culture medium. Background technique [0002] Phalaenopsis, also known as Phalaenopsis, is one of the most widely cultivated and popular species in Orchidaceae, and is known as the "Queen of Orchids". There are more than 40 native species of this genus in the world, and there are 6 native species in my country. However, due to the excessive harvesting of wild resources, most of the wild native species are difficult to find now, and the original species of Phalaenopsis have also been removed. The World Wildlife Conservation Organization lists it as one of the world's top ten endangered plant species. At present, most of the Phalaenopsis ornamental species with various poses and with different expressions on the market and large and colorful flowers are hybrids, and can be hybridized with other genera to form intergenus hybrids, such as intergenus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00A01H4/00
Inventor 曾宋君陈之林段俊陈建通
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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