Paphiopedilum delenatii seed non-symbiotic germination method

A technology of non-symbiotic germination and P. delbrueckii, which is applied in horticultural methods, seed immunization, botanical equipment and methods, etc., can solve the problems of difficult asexual tissue culture, difficult decontamination, and slow propagation speed, etc., to improve germination and growth rate, good seedling quality and high germination rate

Active Publication Date: 2021-06-11
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the ramet propagation of Paphiopedilum derbachie germinates side buds and grows into new seedlings in a natural state, but the propagation speed is slow and the reproduction rate is low; the asexual tissue culture of P. Buds, leaves and roots are propagated as explants, but due to reasons such as difficult decontamination of explants, easy browning and slow proliferation, its asexual tissue culture is extremely difficult; and in sexual reproduction, due to the The seed setting rate in the wild is low, even if the seed germination rate is very low

Method used

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  • Paphiopedilum delenatii seed non-symbiotic germination method

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Experimental program
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Effect test

Embodiment 1

[0093] (1) Artificial pollination and management and protection of capsules

[0094] In the ex situ protection garden, select different individual plants of P. delbrueckii which grow robustly as parents, and carry out artificial pollination of different plants when the flowers are open for 10 days. The pollen sac of the male parent is smeared on the stigma of the female parent, bagged and tagged, and the date of pollination and the traits of the parent are recorded, and then the female plant is artificially cultivated and managed with light, temperature, water, and fertilizer. The pollination success rate reached 100%, and the obtained full capsules were used as materials for the next step of low temperature treatment and aseptic sowing and breeding.

[0095] (2) Low-temperature deposition, disinfection, aseptic sowing and germination culture of capsules

[0096] Harvest mature and plump capsules free of pests and diseases, wrap them in clean paper towels, put them in a box w...

Embodiment 2

[0105] (1) Artificial pollination and management and protection of capsules

[0106] In the ex situ protection garden, different individual plants of P. delbrueckii which grow robustly are selected as parents, and different plants are artificially pollinated when the flowers are open for 15 days. When pollinating, first cut off the pocket of the female parent's lip with scissors, and use a toothpick. The pollen sac of the male parent is smeared on the stigma of the female parent, bagged and tagged, and the date of pollination and the traits of the parent are recorded, and then the female plant is artificially cultivated and managed with light, temperature, water, and fertilizer.

[0107] (2) Low-temperature deposition, disinfection, aseptic sowing and germination culture of capsules

[0108] Harvest mature and plump capsules free from diseases and pests, wrap them in clean paper towels, put them in a box with a lid, cover the box tightly and store them in a desiccator, then pu...

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Abstract

The invention provides a paphiopedilum delenatii seed non-symbiotic germination method, and belongs to the technical field of plant tissue culture. According to the paphiopedilum delenatii seed non-symbiotic germination method, paphiopedilum delenatii seeds are used as explants, and the germination and growth rates of the seeds are improved by performing sowing, differentiation, seedling strengthening and rooting culture on a specific culture medium under a light environment condition; and the germination rate of the seeds is high, the seedling emergence speed is high, the seedling quality is good, and a large number of high-quality paphiopedilum delenatii seedlings are produced within a short time. Experimental results of the embodiment show that the germination rate can reach 47.7% when the paphiopedilum delenatii seeds are cultured for 60 days by the method, the paphiopedilum delenatii seeds can be differentiated into plantlets with the height of about 1.0 cm after 40 days, the plantlets can grow into complete plants with the plant height of 3-4 cm and developed root systems after 60 days, and the transplanting survival rate can reach 100%.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for non-symbiotic germination of Paphiopedilum delbrueckii seeds. Background technique [0002] Paphiopedilum delenatii Guill. is a perennial plant of the genus Paphiopedilum in the Orchidaceae family. It is distributed in northern Guangxi, southeastern Yunnan and northern Vietnam in a very narrow area. Due to the destruction of its habitat and artificial over-excavation, the number of wild plants is extremely rare, and it is listed in Appendix I of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and prohibited from trading, and is also listed by the International Union for Conservation of Nature (IUCN). ) is listed as critically endangered (CR) in the red list and needs to be protected urgently. [0003] On the other hand, Paphiopedilum delbrueckii is one of the most peculiar ornamental orchids. Becau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02A01G24/23A01G24/28A01G24/10A01C1/08
CPCA01H4/001A01H4/008A01H1/02A01G24/23A01G24/28A01G24/10A01C1/08Y02P60/14
Inventor 付传明冼康华苏江何金祥刘宝骏黄宁珍
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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