Phalaenopsis non-toxic seedling cultivation and propagation method
A technology for Phalaenopsis and non-toxic seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as restricting the industrialization development of orchids, affecting quality, necrotic flowers, etc., and achieving low morbidity and seedling quality Good, adaptable effect
Inactive Publication Date: 2017-01-04
成都东山兰韵农业有限公司
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[0003] However, while using tissue culture to bring about rapid reproduction, the spread of the virus is becoming more and more serious. The viruses that seriously harm Phalaenopsis mainly include Jianlan Mosaic Virus and Dental Ringspot Virus. The spread of viruses seriously restricts the industrialization of orchids. The virus can cause symptoms such as leaf spots, necrosis, and flower discoloration and deformity, which seriously affect its quality. At present, there is no definite drug that can effectively prevent the harm and spread of the virus. Therefore, breeding non-toxic seedlings of Phalaenopsis is a must for industrial development. However, the methods for other orchid varieties cannot be directly applied to Phalaenopsis, so it is necessary to independently develop a method for cultivating non-toxic seedlings for Phalaenopsis
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[0018] The specific embodiments of the present invention will be further described below.
[0019] The present invention provides a phalaenopsis non-toxic seedling cultivation and propagation method, thereby cultivating the phalaenopsis non-toxic seedlings and realizing the virus-free cultivation of the phalaenopsis. The method includes the following steps:
[0020] (1) Plant heat treatment: Put the well-growing Phalaenopsis in an incubator for cultivation. During the cultivation period, the humidity in the incubator is 80% to 90%, and the temperature in the incubator starts at 30°C and increases by 1°C every other day. Until 38℃, the incubation time is 22-25d, the light time is 16h / d, and the light intensity is 3000~5000lx; the temperature increases in a stepwise manner. Specifically, the temperature on the first day is 30℃, and the temperature on the second day is 31 ℃, the temperature on the third day is 32℃, and so on, until the temperature on the first day of the ninth day is ...
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The invention relates to the technical field of non-toxic cultivation of plants, in particular to a phalaenopsis non-toxic seedling cultivation and propagation method. The phalaenopsis non-toxic seedling cultivation and propagation method comprises the following steps: performing heat treatment on the plants, selecting materials, disinfecting the materials, performing pre-treatment on the materials, and performing tissue cultivation. The tissue cultivation comprises protocorm induced cultivation, multiplication cultivation, strong seedling cultivation and transplanting. After the phalaenopsis seedlings are cultivated according to the method, the disease incidence rate is low, the survival seedling rate is high, the seedling quality is high, the adaptability of the seedlings is high after being planted, the seedlings grow well, the requirements on commercialized production are met in a short time through a biotechnological means, and technical support is provided for large-scale and industrialized cultivation of the phalaenopsis non-toxic seedlings of enterprises.
Description
Technical field [0001] The invention relates to the technical field of plant non-toxic cultivation, in particular to a method for cultivating and propagating phalaenopsis nontoxic seedlings. Background technique [0002] Phalaenopsis is a famous flower in the current international flower bed. It belongs to the genus Phalaenopsis of Orchidaceae. Its origin is mainly subtropical and tropical, and it is naturally distributed in Oceania and Asia. Phalaenopsis has the characteristics of long viewing period, rich color, beautiful flower color and graceful and light body. It is also called "Queen of Cattleya". It is one of the most popular and widely cultivated species among orchids. Its commercial value is one of the four major ornamental tropical orchids in the world. Phalaenopsis is a single-stem aerial orchid with very few collateral development, so it is difficult to routinely propagate ramets, and it rarely forms seeds. Seed development is also difficult. Basically, it is more di...
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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 苟兴明刘若东
Owner 成都东山兰韵农业有限公司
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