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Dendrobium candidum tissue culture plant hormone control method

A technology of Dendrobium officinale and plant hormones, which is applied in the directions of botanical equipment and methods, plant regeneration, horticultural methods, etc., can solve the problems of shortening the tissue culture time of seedlings, slow proliferation, and low differentiation rate of Dendrobium officinale, and shortens the culture period. , improve the efficiency of cultivation, and optimize the quality of seedlings

Inactive Publication Date: 2012-01-18
姚安农哈哈食用菌开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention mainly aims at improving the quality of tissue culture of Dendrobium candidum, ensuring its high-quality provenance, shortening the time for tissue culture of seedlings, and solving the problems of low differentiation rate and slow proliferation of Dendrobium candidum at present, which cannot satisfy large-scale planting

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] A method for controlling phytohormones in tissue culture of Dendrobium officinale, the present invention cultivates 8 hours / day under 1800LX light at humidity of 70%-80%, temperature 23°C, 1800LX at 23°C, 8 hours / day at 23°C, 400LX at 23°C, 400LX for 8 hours / day Under the condition of hour / day; Dendrobium candidum is cultivated through the following three stages sequentially after routine disinfection procedures:

[0013] 1) Callus culture: put Dendrobium officinale into MS basic medium + 6-benzyl adenine (6BA) 0.25 mg / L + naphthaleneacetic acid (NNA) 0.5 mg / L + sucrose 3% + agar 0.8%, pH value Cultivate for 45 days in the medium of 5.0-6.5 to form protocorms;

[0014] 2) Adventitious bud induction culture: put protocorms through 1 / 2MS basic medium + 6-benzyl adenine (6BA) 0.05 mg / L + naphthalene acetic acid (NNA) 2.0 mg / L + sucrose 2% + agar 0.8%+ Banana juice 20%, cultivated in a medium with a pH value of 5.0-6.5 for 45 days to form seedlings;

[0015] 3) Cultivatio...

Embodiment 2

[0018] A method for controlling phytohormones in tissue culture of Dendrobium officinale, the present invention cultivates 8 hours / day at a humidity of 70%-80%, at a temperature of 28°C, at 2200LX; at 29°C, at 1400LX for 8 hours / day; at 25°C, at 800LX for 8 hours / day Under the condition of hour / day; Dendrobium candidum is cultivated through the following three stages sequentially after routine disinfection procedures:

[0019] 1) Callus culture: put Dendrobium officinale into MS basic medium + 6-benzyl adenine (6BA) 0.25 mg / L + naphthaleneacetic acid (NNA) 0.5 mg / L + sucrose 3% + agar 0.8%, pH value Cultivate in the medium of 5.0-6.5 for 35 days to form protocorms;

[0020] 2) Adventitious bud induction culture: put protocorms through 1 / 2MS basic medium + 6-benzyl adenine (6BA) 0.05 mg / L + naphthalene acetic acid (NNA) 2.0 mg / L + sucrose 2% + agar 0.8%+ Banana juice 20%, cultivated in a medium with a pH value of 5.0-6.5 for 35 days to form seedlings;

[0021] 3) Cultivation ...

Embodiment 3

[0024] A method for controlling phytohormones in tissue culture of Dendrobium officinale, the present invention cultivates 8 hours / day under 2000LX illumination at humidity of 70%-80%, temperature 25°C; 26°C, 1200LX for 8 hours / day; 24°C, 600LX for 8 hours / day Under the condition of hour / day; Dendrobium candidum is cultivated through the following three stages sequentially after routine disinfection procedures:

[0025] 1) Callus culture: put Dendrobium officinale into MS basic medium + 6-benzyl adenine (6BA) 0.25 mg / L + naphthaleneacetic acid (NNA) 0.5 mg / L + sucrose 3% + agar 0.8%, pH value Cultivate for 40 days in the medium of 5.0-6.5 to form protocorms;

[0026] 2) Adventitious bud induction culture: put protocorms through 1 / 2MS basic medium + 6-benzyl adenine (6BA) 0.05 mg / L + naphthalene acetic acid (NNA) 2.0 mg / L + sucrose 2% + agar 0.8%+ Banana juice 20%, cultivated in a medium with a pH value of 5.0-6.5 for 40 days to form seedlings;

[0027] 3) Cultivation of root...

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Abstract

The invention discloses a dendrobium candidum tissue culture plant hormone control method. The method comprises the following steps of: performing culture for 8 hours every day under the conditions that the humidity is 70 to 80 percent, the temperature is 23 to 28 DEG C and the illumination is 1,800 to 2,200LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 29 DEG C and the illumination is 1,000 to 1,400LX; performing culture for 8 hours every day under the conditions that the temperature is 23 to 25 DEG C and the illumination is 400 to 800LX; and after the dendrobium candidum is treated by the conventional sterilization program, sequentially culturing the dendrobium candidum through the following three stages: 1) callus culture; 2) adventitious bud induced culture; and 3) rooted seedling culture. The method has the advantages of greatly improving the dendrobium candidum tissue culture efficiency, optimizing the quality of the seedlings, shortening the culture period and providing a prerequisite for industrialized development of the dendrobium candidum.

Description

technical field [0001] The invention belongs to the technical field of seedling tissue culture, in particular to a method for plant hormone control in dendrobium officinale tissue culture. Background technique [0002] Dendrobium officinale is a perennial epiphytic herb of Orchidaceae. Born on the semi-shady rocks in the mountains at an altitude of 1600 meters, it likes warm and humid climates and semi-shady and semi-yang environments, and is not cold-resistant. Dendrobium can be divided into dozens of species such as yellow grass, nobile, and horsewhip. Dendrobium candidum is the best product of Dendrobium. It is named for its iron-green skin. Dendrobium officinale has unique medicinal value, and its stems are used as medicine. The name of the traditional Chinese medicine: Dendrobium, belongs to the yin-tonifying medicine in the tonic. Or spring-shaped, dried, the commodity is called earrings Dendrobium, has another name called Maple Dou. It is the most precious Chinese ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 滕正平
Owner 姚安农哈哈食用菌开发有限公司
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