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High quality germchit tissure culturing and rapid breeding method of Dendrobium sp.

A technology of tissue culture and dendrobium, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems that there is no patent for the rapid propagation of autumn dendrobium seedlings, and no autumn dendrobium seedlings have been seen. Less investment, strong resistance and high application value

Inactive Publication Date: 2005-06-29
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

At present, there is no report on the rapid propagation of Dendrobium orchid seedlings in tissue culture in China, and there is no patent on the rapid propagation of Dendrobium orchid seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1. Material: Dendrobium butterfly.

[0017] 2. Disinfection of explants: Take the young shoots of Dendrobium nodulii with 5-6 nodes in the same year, remove the leaves, rinse them with tap water, cut them into two sections, soak them in 70% alcohol on the ultra-clean workbench for 1 Minutes, then sterilized with 0.1% mercuric chloride solution for 10 minutes, rinsed with sterile water for 5 times, blotted dry with sterile absorbent paper, cut 2-3 cm segmented stem segments and shoot tips, and inoculated into protocorms and cluster bud induction medium.

[0018] 3. Induction of protocorms and clustered buds: induction medium is MS+6-benzylpurine 2-5 mg / liter+naphthalene acetic acid 0.2-0.5 mg / liter+15% coconut water medium, at culture temperature (26 ±2) Under the condition of ℃, the tip of the stem begins to grow in about 10 days, and new shoots form and grow on the nodes of the stem in about 20 days. When the 6-benzylpurine is 4-5, protocorms are produced at the base ...

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PUM

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Abstract

Disclosed is the high quality germchit tissure culturing and rapid breeding method of Dendrobium sp., wherein a large amount of test tube seedlings with stabilized performance are obtained through fine breed tissue culture by adopting the path of clumping buds. The produced Dendrobium sp. has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and fine growth status.

Description

technical field [0001] The invention relates to a method for rapid propagation of high-quality seedlings of dendrobium orchid and a used culture medium. Background technique [0002] Autumn Dendrobium, also known as Butterfly Dendrobium, is mostly a hybrid variety bred from the parent of the tropical native species Dendrobiumphalaenopsis native to New Guinea. New Guinea and Australia are the hometowns of the original species of autumn Dendrobium. The most cultivated hybrid variety is "Panda No. 1", which is mainly produced in Thailand. Other popular varieties include "Xianti", "Mary", and Norman. [0003] Dendrobium nodica has no obvious dormancy period, and the leaves can last for several years without falling off; inflorescences can be seen coming out from the top of the pseudobulb and the nearby nodes every autumn. The flower stems are relatively long, and each stem has more than ten flowers. Sometimes a pseudobulb can produce multi-branched flowers and bloom continuousl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 李桂双段俊曾宋君陈之林陈建通
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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