High quality germchit rapid breeding method of OncidiumLuridum

Abstract

Disclosed is the high quality germchit rapid breeding method of Oncidium Luridum and culture medium used thereby, wherein a plurality of Oncidium Luridum original breed and hybrid tissue culture breeding methods and unique culture medium employed are provided. The produced Oncidium Luridum germchit has the advantages of high genetic stability, quick emergence of seedlings, strong seedlings, good seedling quality, strong resistance, and good accretion status.

Description

technical field

[0001] The invention relates to a method for rapid propagation of high-quality seedlings of oncidium and the used culture medium. Background technique

[0002] Oncidinm (Oncidinm), also known as Dancing Girl Orchid, King Phalaenopsis, Tubal Orchid, etc., is one of the largest genus in Orchidaceae. There are more than 400 native species of this genus in the world, and it is commercially used in various poses and with different expressions. The species are mostly hybrids. Oncidium is native to the tropical regions of America, and the most widely distributed species are Brazil, the United States, Colombia, Ecuador, and Peru. Its distribution area is relatively wide, there are tropics, warm zone, alpine temperate zone and frigid zone. Oncidium is a very beautiful and highly ornamental orchid. It is one of the important orchid potted flowers and cut flower varieties in the world. When making cut flowers, it is suitable for vases in family rooms and offices. It i...

Examples

Embodiment 1

[0014] 1. Material: Oncidium concolor of the same color

[0015] 2. Disinfection of explants: take the pedicels and stem tips of oncidium flower buds of the same color, rinse them with tap water, cut 2 cm sectioned pedicel segments or newly grown 2 cm young shoots, and place them in an ultra-clean room. Soak in 70% alcohol on the workbench for 1 minute, then sterilize with 0.1% mercuric solution for 10 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the pedicel , cut off 0.3 cm from the base of the young shoots and inoculate them on the protocorm and cluster bud induction medium.

[0016] 3. Induction of pedicel cluster buds: the induction medium of pedicel seedlings is N 6 + 6-benzylpurine 3-5 mg / L + naphthalene acetic acid 0.2-0.5 mg / L + 15% coconut water medium, under the condition of culture temperature (26±2) ℃, flower buds begin to grow in about 15 days, and The fully developed flower buds will contin...

Embodiment 2

[0022] Oncidium Sweet Sugar, a hybrid variety of oncidium, was used as the explant. The basic operation method is consistent with embodiment 1. However, the composition of the medium is slightly different, and the concentration of 6-benzylpurine for the induction and proliferation of its cluster buds is lower than that of the same color oncidium. High concentrations of 6-benzylpurine produce too many protocorms.

[0023] During the cultivation process, the induction medium of pedicel seedlings was N 6 + 6-benzylpurine 1-3 mg / L + naphthalene acetic acid 0.2-0.5 mg / L + 15% coconut water; when shoot tips are used as explants, the induction medium is N 6 + 6-Benzylpurine 1-2 mg / L + Naphthaleneacetic Acid 0.2-0.5 mg / L + 15% Coconut Water. N 6 +6-Benzylpurine 1-3 mg / L+NAA 0.2-0.5 mg / L+15% Coconut Water+Sucrose 30 g / L. Rooting medium is 1 / 2N 6Culture medium + sucrose 15 g / l + banana juice 100 g / l + activated carbon 0.5 g / l, the survival rate of transplanted test-tube plantlets ...

technical field

[0001] The invention relates to a method for rapid propagation of high-quality seedlings of oncidium and the used culture medium. Background technique

[0002] Oncidinm (Oncidinm), also known as Dancing Girl Orchid, King Phalaenopsis, Tubal Orchid, etc., is one of the largest genus in Orchidaceae. There are more than 400 native species of this genus in the world, and it is commercially used in various poses and with different expressions. The species are mostly hybrids. Oncidium is native to the tropical regions of America, and the most widely distributed species are Brazil, the United States, Colombia, Ecuador, and Peru. Its distribution area is relatively wide, there are tropics, warm zone, alpine temperate zone and frigid zone. Oncidium is a very beautiful and highly ornamental orchid. It is one of the important orchid potted flowers and cut flower varieties in the world. When making cut flowers, it is suitable for vases in family rooms and offices. It i...

Examples

Embodiment 1

[0014] 1. Material: Oncidium concolor of the same color

[0015] 2. Disinfection of explants: take the pedicels and stem tips of oncidium flower buds of the same color, rinse them with tap water, cut 2 cm sectioned pedicel segments or newly grown 2 cm young shoots, and place them in an ultra-clean room. Soak in 70% alcohol on the workbench for 1 minute, then sterilize with 0.1% mercuric solution for 10 minutes, rinse with sterile water for 5 times, blot dry with sterile absorbent paper, cut off 0.5 cm at both ends of the pedicel , cut off 0.3 cm from the base of the young shoots and inoculate them on the protocorm and cluster bud induction medium.

[0016] 3. Induction of pedicel cluster buds: the induction medium of pedicel seedlings is N 6 + 6-benzylpurine 3-5 mg / L + naphthalene acetic acid 0.2-0.5 mg / L + 15% coconut water medium, under the condition of culture temperature (26±2) ℃, flower buds begin to grow in about 15 days, and The fully developed flower buds will contin...

Embodiment 2

[0022] Oncidium Sweet Sugar, a hybrid variety of oncidium, was used as the explant. The basic operation method is consistent with embodiment 1. However, the composition of the medium is slightly different, and the concentration of 6-benzylpurine for the induction and proliferation of its cluster buds is lower than that of the same color oncidium. High concentrations of 6-benzylpurine produce too many protocorms.

[0023] During the cultivation process, the induction medium of pedicel seedlings was N 6 + 6-benzylpurine 1-3 mg / L + naphthalene acetic acid 0.2-0.5 mg / L + 15% coconut water; when shoot tips are used as explants, the induction medium is N 6 + 6-Benzylpurine 1-2 mg / L + Naphthaleneacetic Acid 0.2-0.5 mg / L + 15% Coconut Water. N 6 +6-Benzylpurine 1-3 mg / L+NAA 0.2-0.5 mg / L+15% Coconut Water+Sucrose 30 g / L. Rooting medium is 1 / 2N 6Culture medium + sucrose 15 g / l + banana juice 100 g / l + activated carbon 0.5 g / l, the survival rate of transplanted test-tube plantlets ...