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Method for pollination and seed cultivation of fan-shaped oncidium under sterile condition

A fan-shaped oncidium and fan-shaped technology is applied to the fields of pollination and seed cultivation under aseptic conditions of fan-shaped oncidium, can solve problems such as sterility and difficulty in seedling production, achieve simple acquisition, overcome difficulties in cultivation, and be beneficial to factory effect

Inactive Publication Date: 2021-04-09
SOUTH CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fan-shaped oncidium has unique ornamental value and is a very suitable material for ornamental flowers in commercial bottles. However, the fruit formed by artificial pollination under natural conditions often forms sterile and embryoless seeds, which makes it difficult to produce seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) In the ultra-clean bench, use sterilized tweezers to take out the 3-day-flowered Oncidium fancidium plant from the tissue culture bottle, place it in a sterilized tray, take out the pollen block and place it in the small groove of the stigma Inside, pollination is done.

[0026] (2) Transfer to a culture bottle filled with the first culture medium until the ovary begins to expand. The first medium is composed of 3g / L Huabao No. 1, 1mg / L 6-BA, 1mg / L ABA, 40g / L sucrose, 1g / L activated carbon, 4.5g / L agar, pH5.6, the above The raw material is dissolved in deionized water to obtain the first culture medium.

[0027] (3) Take out the plant with the growing ovary in step (2) from the culture bottle, remove all other flower stalks, and transfer it to a culture bottle filled with the second culture medium at a temperature of 25±3°C and a humidity of 30°C. %, light intensity 63±5μmol / m 2 s, the light time was 12h / d and continued to cultivate for 120 days, and the fruit wa...

Embodiment 2

[0030] (1) In the ultra-clean bench, use sterilized tweezers to take out the 7-day-flowered oncidium plant from the tissue culture bottle, place it in a sterilized tray, take out the pollen block and place it in the small groove of the stigma Inside, pollination is done.

[0031] (2) Transfer to a culture bottle filled with the first culture medium until the ovary begins to expand. The first medium is composed of 5g / L Huabao No. 1, 0.5mg / L 6-BA, 1mg / L ABA, 40g / L sucrose, 1g / L gac and 4.5g / L agar, pH5.6, will The above raw materials are dissolved in deionized water to obtain the first culture medium.

[0032] (3) Take out the plant with the growing ovary in step (2) from the culture bottle, remove all other flower stalks, and transfer it to a culture bottle filled with the second culture medium at a temperature of 25±3°C and a humidity of 40°C. %, light intensity 63±5μmol / m 2 s, the light time was 12h / d and continued to cultivate for 110 days, and the fruit was harvested. T...

Embodiment 3

[0035] (1) In the ultra-clean bench, use sterilized tweezers to take out the 3-day-flowered Oncidium fancidium plant from the tissue culture bottle, place it in a sterilized tray, take out the pollen block and place it in the small groove of the stigma Inside, pollination is done.

[0036] (2) Transfer to a culture bottle filled with the first culture medium until the ovary begins to expand. The first medium is composed of 3g / L Huabao No. 1, 1mg / L 6-BA, 1mg / L ABA, 20g / L sucrose, 1g / L activated carbon and 4.5g / L agar, pH5.6, the above The raw material is dissolved in deionized water to obtain the first culture medium.

[0037] (3) Take out the plant with the growing ovary in step (2) from the culture bottle, remove all other flower stalks, and transfer it to a culture bottle filled with the second culture medium at a temperature of 25±3°C and a humidity of 60°C. %, light intensity 63±5μmol / m 2 s, the light time was 12h / d and continued to cultivate for 130 days, and the fruit...

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Abstract

The invention relates to the field of flower cultivation and seedling propagation, and particularly discloses a method for pollination and seed cultivation of fan-shaped oncidium under sterile conditions. The method for pollination and seed cultivation of the fan-shaped oncidium comprises the following steps that after blooming, fan-shaped oncidium flowers are subjected to artificial pollination under sterile conditions; after pollination, the fan-shaped oncidium flowers are transferred into a first culture medium for culture; after ovaries of the fan-shaped oncidium are expanded, the fan-shaped oncidium flowers are transferred to a second culture medium for continuous culture; and a large number of seeds are obtained after fruits are mature. According to the method, mature seeds with high embryo rate and high germination rate are obtained through artificial pollination and fruit development process regulation and control in the culture mediums under sterile conditions, the obtained mature seeds can be used for scientific research and seedling production, and the method has an extremely remarkable promotion effect on fan-shaped oncidium seedling production.

Description

technical field [0001] The invention relates to the fields of flower cultivation and seedling propagation, in particular to a method for pollination and seed cultivation of oncidium fancidium under aseptic conditions. Background technique [0002] Orchids generally have a long vegetative growth period, and most plants need to grow for several years before they can reproduce, which makes the research cycle for orchids long. Oncidium fanatum is widely distributed in southern Mexico, Central America, and the West Indies, and is continuously distributed in the hot and humid forests of Colombia, Ecuador, Peru, Bolivia, Brazil, Venezuela, and Guyana in South America. The number of chromosomes of Oncidium fancidium is 2n=12, and the growth cycle is fast. It can also be used as a model organism of Orchidaceae and play an important role in the biological research of Orchidaceae. However, the huge differences in region, environment and climate make its introduction and cultivation di...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G31/00
CPCA01G31/00A01H1/02
Inventor 马得草刘伟张婷林锐松张菁
Owner SOUTH CHINA AGRI UNIV
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